Itgam (NM_008401) Mouse Untagged Clone

CAT#: MC223731

Itgam (untagged) - Mouse integrin alpha M (Itgam), transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Itgam
• Synonyms: Cd11b; CD11b/CD18; CR3; CR3A; F730045J24Rik; Ly-40; Mac-1; Mac-1a; MAC1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC223731 representing NM_008401
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACTCTTAAAGCTCTTCTGGTCACAGCCCTAGCCTTGTGTCATGGCTTCAATCTGGACACTGAACATC
  CCATGACCTTCCAAGAGAATGCAAAAGGCTTTGGACAGAGTGTGGTCCAGCTTGGCGGAACCAGTGTGGT
  TGTTGCAGCCCCCCAGGAGGCAAAGGCTGTTAACCAGACAGGTGCCCTCTACCAGTGTGACTACAGCACA
  AGCCGGTGTCACCCCATCCCCCTGCAAGTACCTCCAGAGGCTGTGAATATGTCCTTGGGCCTGTCCCTGG
  CTGTTTCTACTGTCCCCCAGCAGCTGCTGGCCTGTGGCCCCACGGTGCACCAAAACTGCAAGGAGAATAC
  TTATGTGAATGGATTGTGCTATTTGTTCGGCTCCAACCTGCTGAGGCCGCCCCAGCAGTTCCCAGAGGCT
  CTCAGAGAATGTCCTCAGCAGGAGAGTGACATTGTCTTCTTGATTGATGGCTCCGGTAGCATCAACAACA
  TTGACTTTCAGAAGATGAAGGAGTTTGTCTCAACTGTGATGGAGCAGTTCAAAAAGTCTAAAACCTTGTT
  CTCTTTGATGCAGTACTCGGACGAGTTCCGGATTCACTTCACCTTCAATGACTTCAAGAGAAACCCTAGC
  CCAAGATCACATGTGAGCCCCATAAAGCAGCTGAATGGGAGGACAAAAACTGCCTCAGGGATCCGGAAAG
  TAGTGAGAGAACTGTTTCACAAAACCAATGGGGCCCGGGAGAATGCTGCGAAGATCCTAGTTGTCATCAC
  AGATGGAGAAAAATTCGGTGATCCCTTGGATTATAAGGATGTCATCCCCGAGGCAGACAGAGCAGGGGTC
  ATTCGCTACGTAATTGGGGTGGGAAATGCCTTCAACAAACCACAGTCCCGCAGAGAGCTCGACACCATCG
  CATCTAAGCCAGCTGGTGAACACGTGTTCCAAGTGGACAACTTTGAAGCCCTGAATACCATTCAGAACCA
  GCTTCAGGAAAAGATCTTTGCAATTGAGGGCACGCAGACAGGAAGTACCAGCTCCTTTGAGCATGAGATG
  TCTCAAGAAGGCTTCAGTGCTTCCATTACCTCTAATGGTCCCTTGCTGGGCTCTGTGGGGAGCTTTGACT
  GGGCAGGTGGAGCCTTCCTGTATACATCGAAGGATAAAGTCACCTTCATCAACACAACCAGAGTGGATTC
  AGACATGAATGATGCTTACCTGGGTTATGCTTCTGCAGTCATCTTGAGGAACCGTGTCCAAAGCTTGGTT
  TTAGGAGCACCTCGGTATCAGCATATTGGCTTGGTGGTGATGTTCAGGGAGAATTTCGGTACCTGGGAGC
  CCCACACTAGCATCAAGGGCAGCCAGATTGGCTCTTATTTTGGGGCCTCCCTTTGCTCTGTGGACATGGA
  CGCTGATGGCAATACCAACTTGATCCTCATTGGGGCCCCTCATTACTATGAGAAGACCCGAGGAGGCCAG
  GTGTCAGTGTGTCCCTTGCCTCGAGGGAGGGCACGGTGGCAGTGTGAAGCTCTTCTCCACGGTGATCAGG
  GTCATCCCTGGGGTCGCTTTGGGGCAGCCCTGACAGTGCTGGGAGACGTGAATGGGGACAAACTGACAGA
  TGTGGCCATTGGGGCCCCAGGAGAGCAGGAGAATCAGGGCGCTGTCTACATTTTTTATGGAGCATCAATA
  GCCAGCCTCAGTGCCTCCCACAGCCAGCGGATCATAGGCGCCCACTTCTCCCCTGGGCTCCAGTACTTCG
  GGCAGTCTCTGAGTGGGGGTAAGGATCTCACAATGGATGGCTTGATGGACCTGGCTGTTGGGGCGCAGGG
  GCATCTTCTGCTGCTCAGAGCCCAGCCGGTGCTGAGACTGGAGGCAACCATGGAATTCAGCCCCAAGAAA
  GTAGCAAGGAGTGTGTTTGCATGTCAAGAACAAGTATTAAAAAACAAGGATGCTGGGGAGGTCAGAGTCT
  GCCTCCGTGTCCGCAAGAACACCAAGGACCGTCTGCGCGAAGGAGATATCCAGAGCACTGTCACTTATGA
  CCTGGCTTTAGACCCTGGCCGCTCACGTATCCGTGCCTTCTTTGATGAGACAAAGAACAACACACGCAGG
  CGCACCCAGGTCTTTGGATTGATGCAGAAATGTGAAACACTGAAGCTAATTTTACCGGACTGCGTGGACG
  ACTCAGTGAGCCCCATCATCCTGCGCCTCAATTATACACTGGTTGGGGAGCCCTTGAGGTCCTTTGGGAA
  CCTCCGACCAGTTCTGGCTATGGATGCTCAGAGGTTCTTCACAGCTATGTTTCCCTTTGAGAAAAATTGC
  GGCAATGACAGCATCTGCCAAGACGATCTCAGCATCACCATGAGTGCCATGGGCTTGGACACTTTGGTGG
  TGGGAGGCCCCCAGGACTTTAACATGAGTGTGACTCTGAGAAATGACGGTGAGGATTCCTACGGGACCCA
  GGTTACCGTCTACTACCCATCTGGCTTATCTTACCGGAAGGATTCAGCAAGCCAGAACCCGCTCACCAAG
  AAGCCTTGGTTTGTAAAGCCTGCTGAGTCCAGCTCTTCTTCTGAAGGGCATGGGGCTCTGAAGAGTACCA
  CCTGGAACATAAACCATCCCATCTTTCCTGCTAATTCTGAGGTCACATTTAATGTCACATTTGATGTGGA
  CTCTCATGCCTCCTTTGGGAACAAACTGCTCCTCAAAGCCATTGTGGCCAGTGAGAACAACATGTCCAGG
  ACCCACAAAACCAAGTTTCAGTTGGAGCTGCCTGTGAAGTACGCCATCTACATGATTGTCACCAGTGATG
  AGAGTTCTATCAGATATCTCAACTTCACGGCTTCAGAGATGACCAGTAAGGTCATACAGCATCAGTACCA
  GTTCAACAACCTGGGCCAGAGGAGCCTCCCTGTCAGTGTAGTCTTCTGGATCCCTGTTCAGATCAACAAT
  GTGACCGTATGGGATCATCCCCAGGTCATCTTCTCCCAGAACCTCTCAAGTGCCTGTCACACTGAGCAGA
  AATCCCCCCCTCACTCCAATTTCCGGGATCAGCTTGAAAGGACCCCAGTGCTGAACTGCTCTGTTGCAGT
  CTGTAAGAGAATTCAGTGTGACCTTCCATCCTTCAACACTCAGGAAATATTCAATGTCACCCTCAAGGGC
  AACCTATCATTTGACTGGTACATCAAGACTTCTCATGGTCACCTCCTGCTTGTGAGCAGCACTGAGATCC
  TGTTTAATGACTCTGCGTTTGCCCTGCTTCCAGGGCAGGAGTCGTATGTGAGGTCTAAGACAGAGACCAA
  AGTGGAGCCATATGAAGTTCACAATCCTGTACCACTCATTGTGGGCAGCTCCATTGGGGGCCTGGTGCTC
  TTGGCTCTCATCACTGCTGGCCTATACAAGCTTGGCTTTTTCAAGCGGCAGTACAAGGACATGATGAATG
  AAGCTGCCCCCCAAGACGCCCCACCTCAGTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_008401
• Insert Size: 3462 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_008401.2, NP_032427.2
• RefSeq Size: 4679 bp
• RefSeq ORF: 3462 bp
• Locus ID: 16409
• Cytogenetics: 7 F3