Pelp1 (NM_029231) Mouse Untagged Clone

CAT#: MC223651

Pelp1 (untagged) - Mouse proline, glutamic acid and leucine rich protein 1 (Pelp1), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Pelp1
• Synonyms: 4930563C04Rik; MNAR
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC223651 representing NM_029231
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGCGGCCGTTCTTAGTGGGGCCTCTGCGGGCTCCCCAGCTGGGGCTCCTGGCGGGCCCGGGGGTC
  TCTCTGCGGTGGGATCCGGCCCGCGTCTGCGTCTGCTGCTGTTAGAGAGCATTTCCGGGTTGTTACAGCC
  GCGAACGGCGTCCCCCGTGGCCCCTGTGCATCCCCCAATCCAGTGGGCCCCGCATTTGCCCGGGCTCATG
  TGCTTATTGAGGCTGCATGGGACGGCAGGTGGGGCGCAGAATCTTTCAGCTCTTGGGGCATTGGTGAATC
  TCAGTAATGCACATCTTGGCTCCATCAAAACTCGGTTTGAAGGCCTGTGTCTGCTGTCCTTGTTGATAGG
  GGAGAGCCCCACAGAGTTATTCCAGCAACATTGTGTTTCTTGGCTTCGGAGCATCCAGCAGGTGCTGCAG
  TCTCAGGACTCACCGTCCACCATGGAGCTGGCTGTGGCTGTCCTGAGGGATCTGCTCCGACATGCATCCC
  AGCTTCCTACCCTGTTTCGGGACATCTCCACCAACCACCTTCCTGGGCTTCTTACTTCCTTGCTGGGCCT
  CAGACCAGAGTGTGAACAATCAGCTTTGGAGGGAATGAAGGCTTGTGTGACCTATTTTCCTCGGGCCTGT
  GGCTCTCTAAAAGGAAAGCTGGCTTCCTTTTTCCTGTCTCGATTGGATTCCCTGAACCCTCAGCTCCAAC
  AGCTGGCCTGCGAGTGTTACTCCAGGTTGCCCTCGTTAGGTGCTGGCTTTTCCCAGGGACTGAAGCACAC
  AGAGAACTGGGAACAGGAGCTGCACAGCCTGCTGACCTCACTGCACAGCTTACTGGGGTCCCTGTTTGAG
  GAAACAGAGCCTGCTCCTGTGCAGAGTGAAGGCCCTGGAATAGAGATGCTGCTTTCCCACTCAGAAGATG
  GTAACACCCATGTCCTCCTCCAGCTTCGGCAGAGGTTTTCAGGCCTGGCCCGTTGCCTGGGGCTCATGCT
  CAGCTCTGAGTTTGGGGCTCCTGTGTCCGTTCCTGTGCAGGAAATCCTGGACCTCATCTGCCGGATCCTT
  GGTATCAGTAGCAAGAATATTAACTTGCTTGGAGATGGTCCCCTCAGGTTGCTGCTGCTGCCTTCTCTGC
  ACCTTGAGGCCTTGGACTTGCTCTCTGCCCTGATCCTTGCGTGTGGAAGTCGACTTTTGCGTTTTGGTGC
  CCTGATCAGCCGGCTGCTTCCCCAGGTTCTCAATGCCTGGAGCACTGGGAGGGATACTCTAGCTCCAGGC
  CAGGAGAGGCCTTACAGCACCATTCGGACCAAAGTGTATGCTATCTTAGAGCTATGGGTGCAGGTTTGTG
  GGGCCTCAGCAGGTATGCTTCAGGGAGGAGCCTCTGGAGAAGCCTTGCTCACCCACCTGCTCAGTGACAT
  CTCCCCTCCAGCTGATGCTCTTAAGCTATGCAGTACCAGGGGAAGCTCTGATGGAGGTTTGCAAAGTGGG
  AAGCCAAGTGCTCCTAAGAAGCTCAAGCTCGATATGGGGGAAGCTTTGGCTCCACCCAGCCAAAGGAAAG
  GAGATAGGAATGCCAACAGTGACGTGTGCGCAGCTGCACTGAGAGGCCTCAGTCGGACCATCCTTATGTG
  CGGGCCTCTCATCAAGGAGGAGACTCACAGGAGACTTCATGATCTAGTCCTGCCCCTGGTCATGAGTGTC
  CAGCAGGGTGAGGTCCTGGGCAGCTCTCCTTACAACAGTTCCTGCTGTCGCCTCGGGCTCTACCGCCTGC
  TGCTGGCTTTATTGCTGGCACCTTCCCCTCGGTGCCCTCCTCCTCTTGCCTGTGCCCTGAAAGCTTTCTC
  TCTTGGCCAATGGGAAGATAGTCTTGAGGTCTCCTCTTTTTGCTCAGAAGCTCTGGTGACCTGTGCTGCT
  CTGACACACCCCCGAGTACCTCCTCTACAGAGCTCAGGCCCTGCCTGCCCCACTCCTGCCCCCGTCCCTC
  CTCCTGAGGCCCCATCTTCATTCAGGGCCCCGGCCTTCCATCCTCCAGGCCCCATGCCCTCCATAGGTGC
  TGTGCCATCCACAGGCCCATTACCCTCAGCAGGCCCCATTCCCACAGTAGGCTCTATGGCGTCCACAGGC
  CAAGTGCCCTCTCGCCCTGGACCTCCAGCAACAGCCAACCATTTAGGCCTCTCTGTCCCAGGCCTGGTAT
  CTGTTCCACCCAGGCTCCTTCCTGGCCCTGAAAACCACCGTGCAGGCTCCGGTGAGGATCCTGTCCTTGC
  TCCTAGTGGGACCCCTCCGCCTAGCATACCTCCAGATGAAACTTTTGGGGGGAGAGTACCTAGGCCAGCC
  TTTGTCCACTACGATAAGGAGGAGGCATCTGATGTAGAGATCTCCTTGGAAAGCGACTCTGATGACAGTG
  TGGTGATTGTACCTGAGGGGCTTCCCTCCCTGCCACCTGCACCACCCTCAGGCACTCCTCCCCCTGCGGC
  TCCTGCCGGCCCACCAACAGCCTCACCTCCTGTGCCAGCTAAGGAGGATTCTGAAGAACTGCCTGCAACC
  CCAGGGCCTCCACCCCCACCCCCACCCCCACCCCCACCGGCCTCTGGTCCTGTGACACTTCCTCCACCCC
  AGCTGGTCCCTGAAGGGACTCCTGGAGGGGGAGGACCCACAGCCATGGAAGAAGATTTGACAGTTATTAA
  TATCAACAGCAGTGATGAAGAGGAGGAGGAGGAAGAGGAAGAGGAGGAAGAAGATGAAGATGAGGAAGAA
  GAAGACTTTGAGGAAGAGGAAGAAGATGAAGAAGAATATTTTGAAGAGGAAGAAGAGGAAGAAGAGTTTG
  AGGAGGAATTTGAGGAGGAAGAAGGTGAATTAGAAGAGGAAGAAGAAGAGGAGGAAGAAGAGTTAGATGA
  GGTAGAAGATGTGGAGTTTGGTTCAGCAGGGGAGGTGGAAGAAGGTGGACCTCCACCCCCAACACTGCCA
  CCTGCTCTGCCACCTTCAGACTCGCCCAAAGTACAGCCTGAGGCAGAACCTGAACCTGGGCTCTTATTGG
  AAGTTGAGGAGCCAGGGCCAGAGGAGGTGCCTGGGCCTGAGACTGCTCCTACCCTGGCCCCTGAAGTGCT
  CCCCTCCCAGGAGGAGGGGGAGCAGGAAGTGGGAAGCCCTGCAGCAGGGCCACCTCAGGAGCTTGTTGAA
  GAGTCCTCTGCTCCCCCTGCCCTGCTGGAAGAGGGGACTGAGGGTGGAGGTGACAAAGTGCCACCCCCAC
  CGGAGACACCTGCAGAAGAAATGGAGACAGAAGCAGAAGTTCCAGCTCCTCAGGAAAAGGAGCAAGATGA
  TACAGCTGCCATGCTGGCTGATTTCATCGATTGTCCCCCTGACGATGAGAAGCCACCACCTGCTACAGAG
  CCTGACTCGTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_029231
• Insert Size: 3372 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_029231.4, NP_083507.3
• RefSeq Size: 3463 bp
• RefSeq ORF: 3372 bp
• Locus ID: 75273
• UniProt ID: Q9DBD5
• Cytogenetics: 11 B3
• Gene Summary: Coactivator of estrogen receptor-mediated transcription and a corepressor of other nuclear hormone receptors and sequence-specific transcription factors. Plays a role in estrogen receptor (ER) genomic activity when present in the nuclear compartment by activating the ER target genes in a hormonal stimulation dependent manner. Can facilitate ER non-genomic signaling via SRC and PI3K interaction in the cytosol. Plays a role in E2-mediated cell cycle progression by interacting with RB1. May have important functional implications in ER/growth factor cross-talk. Interacts with several growth factor signaling components including EGFR and HRS. Involved in nuclear receptor signaling via its interaction with AR and NR3C1. May promote tumorigenesis via its interaction with and modulation of several oncogenes including SRC, PI3K, STAT3 and EGFR. Plays a role in cancer cell metastasis via its ability to modulate E2-mediated cytoskeleton changes and cell migration via its interaction with SRC and PI3K (By similarity). Functions as the key stabilizing component of the Five Friends of Methylated CHTOP (5FMC) complex; the 5FMC complex is recruited to ZNF148 by methylated CHTOP, leading to desumoylation of ZNF148 and subsequent transactivation of ZNF148 target genes (PubMed:22872859). Component of the PELP1 complex involved in the nucleolar steps of 28S rRNA maturation and the subsequent nucleoplasmic transit of the pre-60S ribosomal subunit. Regulates pre-60S association of the critical remodeling factor MDN1 (By similarity).[UniProtKB/Swiss-Prot Function]