Usp36 (NM_001033528) Mouse Untagged Clone

CAT#: MC223559

Usp36 (untagged) - Mouse ubiquitin specific peptidase 36 (Usp36), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Usp36
• Synonyms: 2700002L06Rik; mKIAA1453
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC223559 representing NM_001033528
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCTATAGTCGATAAGCTGAAGGAGGCTCTCAAGCCTGGCCGCAAGGACTCGGCCGAGGATGGGGACC
  TGGGCAGGCTGCTGGCAGCCTCTGCCAAGAAGGTGCTTCTGCAGAGGATTGAGTTTGAGCCGGCCAGCAA
  GAGCTTCTCCTACCAGCTGGAGTCCTTGAAGAGCAAATACGTACTGTTGAGCGCCAGGGCTGAGGGAGCC
  AGTCGCCACAGGAGCGGAGATGAACTCCAGGCCAGGAAACCAGGCACCGAGCGTGTGTCTGGAAGTGGAG
  GCGACGGAGTCCCTGCCCCGCAGAAAGTGCTCTTCCCTGTGGAGCGTCTGTCCCTGAGGTGGGAACGTGT
  TTTCCGAGTTGGCGCAGGGCTGCACAACCTGGGTAACACCTGCTTCCTCAACTCCACCATCCAGTGCTTG
  ACCTACACACCACCTCTGGCCAACTACTTGCTCTCCAAGGAGCACGCACGGAGCTGTCACCAAGGGGGCT
  TCTGCATGCTGTGTCTCATGCAAAACCACATGGTCCAGGCCTTTGCCAACAGCGGCAATGCTATCAAGCC
  CGTCTCCTTCATCAGAGACCTGAAAAAGATTGCCCGGCACTTCCGGTTCGGGAACCAAGAGGATGCACAC
  GAATTCCTGCGGTACACCATTGATGCGATGCAGAAGGCCTGCTTGAATGGCTACGCTAAGTTGGATCGGC
  AGACACAGGCTACTACCCTGGTGCATCAGATCTTTGGAGGCTATCTCAGGTCCCGAGTGAAGTGCTCTGT
  GTGCAAGAGTGTCTCAGACACATACGATCCCTACTTGGACATAGCACTGGAGATCCGGCAAGCTGCAAAT
  ATTGTGCGCGCTCTGGAACTTTTTGTGAAGTCAGATGTCCTGAGTGGAGAGAACGCCTATATGTGTGCTA
  AGTGTAAGAAGAAGGTTCCAGCCAGCAAGCGCTTCACCATCCATAGAACATCCAATGTCCTGACTCTGTC
  CCTCAAGCGCTTCGCCAACTTTAGTGGGGGGAAGATCACCAAGGATGTTGGCTATCCAGAGTTCCTGAAC
  ATCCGTCCATACATGTCGCAGAGCAGCGGTGATCCTGTCATGTATGGGCTCTATGCTGTCCTGGTGCACT
  CAGGCTACAGCTGTCACGCTGGGCACTACTACTGCTATGTGAAGGCCAGCAATGGACAGTGGTACCAGAT
  GAACGACTCCTTGGTCCATTCCAGCAATGTCAAGGTGGTTCTGAACCAGCAGGCCTACGTGCTCTTCTAT
  CTGCGGATTCCAGGCTCTAAGAAAAGTCCTGAGGGCCCTGTCTCCAGAGTGGGCGCCACTCTCCCTAGCC
  GTCCCAAAGTTGTTCCAGAGCACTCCAAGAAGAGCCCTGGCAATGGGGTTGTTCCCTCCCCGCTGATGGC
  AAAGCGACAAGACTCTGTAATGATGAGAAAGCTCCCAGCCCCTGAGGAGGTTGGCGTACCTGTGTCCAGA
  AATGGCTCCCTTCCTGGCCTGAAGTTACAGAACGGATGTGCTCCTGCCAAGACACCTGCCGGGTCCCCGT
  CCCCAAGACTCACGCCCACGCCCACTCATATGCCCACCATTCTGGATGAGCCCGGAAAGAAGGTGAAGAA
  GTCAGCCCCACTGCAGTCTCTCACCACATCTCCAACCACTTCTCAGGGCTCTCCTGGGACCGGCGAGTCC
  CGGAGCCAGCGGCCCGGCTCCTGGGCAAGCAGAGACACCATCTTCTCTACCTCCCCGAAGCTCCTGGCCA
  GAGCCATTACTAACGGGCACAGGCTGAAGGGCGAAGGCAGTGGTGTGGACCTGGAGAAGGGGGATTCAAG
  CAGCTCCAGCCCCGAGCACTCTGCCAGCAGTGACCCTGCCAAGGCCCCGCAGACCGCAGAGAGCAGAGCT
  GCGCATGCCTGTGATTCTCAGGGAACAAACTGTCCCACCGCTGGCCATCCCAAAGCGCTGTTGAATGGAG
  TAGATGCCAAGATGGTGAAGTTGAAGTCCCCGGCCCTGAGCAGCACCACAACCGAGCCCACAAGCCTCAT
  GTCTCCTCCACCAGCCAAAAAACTGGCCCTGTCAGCCAAGAAGGCCAGCACCCTGCGGAGGGCGACCGGC
  AATGACATCGGTTCACCTTCCCCCTCAGCATTCTGCGACCTCACCTCCCCCATGAAAGCCACCCACCCCG
  TCGTTGCCTCCACTGGGCCTGTCAGTAAGACCAGGACTGCTGCACCTGCCCCCCGACCGTCCACCCACCC
  CCACTCTGCATCTCTGTCCAGTAGTAGTGCCAAGCCCTTAGGGACATCAGAGCCACAGAGCTGCCGCCCC
  TCTGCCTGGACACCCCTGCCTCAGGTCAATGGACACTTCACGTCCCACTTACACCAACTGCCAGAGGCCA
  GCGAGGCCCTACACAGCCCCTCCAAAAAGAGGAAAAAGACCCCTAATGGAGATCCCCAGAGACTGGGCAT
  CGACACGCTCCTCCCACAGTGCCTCAGGGGAGCACCTGCAGCAGCACGCAGGAAGAGAAAGAAGAGGTGC
  TCGGAGGGTGAGGGTGCCACAGCTCCCAAGCAGGAAGGCCAATTTCAGGACCAGTCTTGGAGTTCCGGGA
  GCCAGAAGGAAGAGGGTACGCAGCCCCAGGTAAATGGCCATCAAGTGAGTCACATTCTGGACAGTTACCA
  TGTGAGCAGCAGGAAAAGGAGGAAGAGGAAAAGATCGGAAGGACTCAGCCAGGAAGCCACCCCGTCGCAG
  GACCTAATTCAGCACAGCTGCTCCCCTGTAGACCACAGTGAGCCTGAGGCCAGGACAGAGTTGCAGAAGA
  AGAAGAAGAAGAAAAGAAGGAAAAGAAAGCCCGAGCCACAGCAGGATGAAGAGAGCAAGCACCCAGGAGA
  CCAGAGGAGCCCAAGGCCCAGTGTCACCCCAGTCCCTGCGTTGAGTGTGAATGGCCATCTTCCTAGTGAC
  TGCTTGGGGCTGGGACAAGCACCTCTCGTTACCTGGAACAGAGATCAGGAACCCGATGTGGTCCAGGCGT
  TGCTTCAGGACTCATCTGATAAGGCTTATGGGAAAAAAGTTTTGACCTGGGATGGCGAGCCTTCAGCCAT
  CAGTCAGGACGCCATCAAAGACAGCAGACTAGCCCGGACCCAGACCGTGGTTGACGACTGGGATGAAGAG
  TTTGATCGAGGGAAGGAAAAGAAAATTAAAAAGTTCAAGAGAGAAAAGAAGAGAAACTTCAATGCCTTCC
  AGAAGCTTCAGAGTAGGCGCAACTTTTGGTCTGTGACGCATCCTGCTAAGGTGGCCAGCCTCAGCTATCG
  CCGCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001033528
• Insert Size: 3297 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001033528.1, NP_001028700.1
• RefSeq Size: 5609 bp
• RefSeq ORF: 3297 bp
• Locus ID: 72344
• UniProt ID: B1AQJ2
• Cytogenetics: 11 E2
• Gene Summary: Deubiquitinase essential for the regulation of nucleolar structure and function. Required for cell and organism viability. Plays an important role in ribosomal RNA processing and protein synthesis, which is mediated, at least in part, through deubiquitination of DHX33, NPM1 and FBL, regulating their protein stability (PubMed:29273634). Function as a transcriptional repressor by deubiquiting histone H2B at the promoters of genes critical for cellular differentiation, such as CDKN1A, thereby preventing histone H3 'Lys-4' trimethylation (H3K4). Specifically deubiquitinates MYC in the nucleolus, leading to prevent MYC degradation by the proteasome: acts by specifically interacting with isoform 3 of FBXW7 (FBW7gamma) in the nucleolus and counteracting ubiquitination of MYC by the SCF(FBW7) complex. In contrast, it does not interact with isoform 1 of FBXW7 (FBW7alpha) in the nucleoplasm. Interacts to and regulates the actions of E3 ubiquitin-protein ligase NEDD4L over substrates such as NTRK1, KCNQ2 and KCNQ3, affecting their expression an functions. Deubiquitinates SOD2, regulates SOD2 protein stability. Deubiquitinase activity is required to control selective autophagy activation by ubiquitinated proteins (By similarity).[UniProtKB/Swiss-Prot Function]