Ipo5 (NM_023579) Mouse Untagged Clone

CAT#: MC223537

Ipo5 (untagged) - Mouse importin 5 (Ipo5), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Ipo5
• Synonyms: 1110011C18Rik; 5730478E03Rik; AA409333; C76941; IMB3; Kpnb3; Ranbp5
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC223537 representing NM_023579
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGCGGCCGCGGCGGAGCAGCAACAGTTCTACCTGCTCCTGGGAAACCTGCTCAGCCCCGACAATG
  TGGTCCGGAAACAGGCGGAGGAAACCTATGAGAATATCCCAGGCCGGTCCAAGATCACATTCCTCTTACA
  AGCCATCAGAAACACAACCGCTGCCGAGGAGGCTAGACAAATGGCTGCTGTTCTCCTAAGACGTCTTTTG
  TCCTCTGCATTTGATGAAGTCTACCCAGCTCTTCCATCAGATGTCCAGACTGCCATCAAGAGTGAATTGC
  TAATGATCATTCAGATGGAAACACAATCCAGCATGAGGAAGAAAATCTGTGATATTGCTGCAGAATTGGC
  CAGGAATTTAATAGATGAGGACGGCAACAACCAGTGGCCTGAAGGTTTGAAGTTCCTCTTTGATTCAGTC
  AGCTCACAAAACATGGGACTCCGGGAAGCTGCCCTTCACATATTTTGGAACTTTCCTGGAATTTTTGGGA
  ACCAACAACAGCACTATTTGGACGTCATCAAACGGATGTTAGTTCAGTGTATGCAAGATCAGGAGCATCC
  TTCGATCAGGACACTGTCTGCCAGAGCTACTGCTGCCTTCATACTTGCCAATGAGCATAATGTCGCTCTG
  TTCAAACACTTTGCAGACTTGCTGCCTGGATTCTTACAGGCTGTCAATGACTCATGCTACCAGAATGATG
  ACTCGGTCCTAAAATCCCTTGTTGAGATTGCAGACACCGTACCAAAGTACTTGCGCCCTCACTTAGAAGC
  AACTCTGCAGTTGAGTCTGAAGTTGTGTGGAGACACTAACCTCAATAATATGCAGCGCCAGCTTGCCCTT
  GAAGTGATTGTGACACTGTCAGAGACTGCAGCTGCTATGTTAAGAAAGCATACCAGTCTCATCGCACAGA
  CGATTCCTCAGATGTTGGCAATGATGGTTGACCTAGAAGAAGATGAGGACTGGGCAAATGCTGATGAGCT
  AGAAGATGATGATTTCGATAGCAATGCAGTTGCTGGTGAGAGTGCATTAGACCGAATGGCCTGTGGACTT
  GGTGGAAAGCTCGTTCTGCCCATGATTAAGGAACACATTATGCAAATGCTTCAAAACCCTGATTGGAAAT
  ACAGGCATGCAGGACTGATGGCCCTATCCGCCATTGGCGAAGGATGCCACCAGCAGATGGAAGGAATTCT
  AAATGAGATTGTAAATTTTGTCTTGCTTTTCCTCCAGGATCCTCATCCGAGAGTCCGGTATGCAGCCTGC
  AATGCTGTGGGTCAAATGGCTACAGATTTTGCACCTGGTTTTCAAAAGAAATTTCATGAGAAGGTGATTG
  CAGCTTTGCTTCAGACCATGGAAGACCAAGGCAACCAGCGGGTGCAGGCCCATGCAGCGGCTGCCCTCAT
  TAACTTCACTGAAGATTGTCCCAAGTCGCTGCTTATTCCCTACTTGGATAACTTGGTGAAGCACCTGCAC
  TCCATTATGGTGCTCAAGCTCCAGGAGCTGATTCAGAAAGGCACCAAGTTAGTTTTGGAACAAGTTGTGA
  CATCCATTGCATCAGTTGCAGATACTGCAGAAGAAAAGTTTGTCCCCTACTATGACTTGTTTATGCCATC
  ACTGAAGCACATTGTCGAGAACGCAGTCCAGAAGGAGCTGAGACTGCTGCGAGGGAAGACCATCGAGTGC
  ATCAGCCTCATCGGGCTGGCCGTCGGGAAGGAGAAATTCATGCAGGATGCTTCAGATGTGATGCAGCTAT
  TGTTGAAGACACAGACAGACTTCAATGATATGGAAGATGACGACCCCCAGATTTCTTACATGATCTCAGC
  ATGGGCCAGGATGTGCAAAATCCTTGGGAAAGAATTCCAGCAGTACCTTCCCGTGGTTATGGGGCCGCTG
  ATGAAGACTGCTTCAATTAAGCCTGAAGTGGCCCTTCTAGACACCCAGGACATGGAGAATATGAGTGATG
  ATGACGGTTGGGAATTTGTGAACCTCGGAGATCAGCAAAGTTTTGGTATTAAAACTGCAGGACTGGAAGA
  AAAGTCAACGGCTTGTCAGATGTTGGTTTGCTATGCTAAGGAATTAAAGGAAGGTTTTGTGGAATACACT
  GAACAGGTTGTCAAACTGATGGTCCCGCTGCTGAAATTTTATTTCCACGATGGGGTCCGAGTGGCAGCAG
  CAGAGTCCATGCCTCTTCTGCTTGAGTGTGCGAGAGTCCGGGGTCCCGAGTACCTTACACAGATGTGGCA
  CTTCATGTGCGACGCTCTCATCAAGGCCATCGGCACAGAGCCCGACTCAGATGTCCTCTCGGAGATCATG
  CATTCCTTTGCAAAGTGCATTGAGGTCATGGGAGACGGGTGCCTCAACAATGAGCACTTTGAGGAGCTGG
  GAGGCATCCTGAAGGCGAAGCTTGAAGAACATTTCAAAAATCAGGAGTTGCGGCAAGTTAAAAGACAAGA
  TGAAGACTATGACGAGCAGGTTGAAGAGTCACTACAAGATGAGGATGATAATGATGTTTATATACTGACT
  AAAGTGTCGGATATTTTACACTCAATATTCAGTAGCTACAAAGAAAAGGTGTTGCCGTGGTTTGAACAGC
  TGCTCCCATTAATTGTCAACCTGATTTGTCCGCAAAGACCATGGCCAGACAGACAATGGGGATTGTGCAT
  CTTCGATGATATCGTAGAACACTGTAGTCCAGCTTCATTTAAGTATGCAGAATATTTCATAAGTCCAATG
  CTCCAGTATGTGTGTGACAACAGCCCAGAAGTCAGGCAAGCTGCGGCATATGGCCTCGGCGTCATGGCGC
  AGTTTGGTGGAGATAACTACCGCCCTTTCTGTACCGATGCACTCCCGCTGCTTGTAAGAGTCATTCAGGC
  TCCAGAGGCTAAGACCAAAGAAAATGTCAACGCCACGGAGAACTGCATCTCAGCAGTGGGAAAGATCATG
  AAGTTCAAGCCCGACTGTGTGAATGTGGAGGAGGTCTTACCACACTGGCTGTCATGGCTGCCCCTACACG
  AAGATAAAGAAGAAGCTGTCCAGACCTTCAGTTACCTGTGCGACCTGATTGAAAGTAATCACCCAATTGT
  TCTTGGCCCAAACAATACCAATCTACCGAAAATATTCAGTATAATTGCAGAAGGAGAAATGCATGAGGCC
  ATTAAACATGAAGACCCCTGTGCCAAACGCCTGGCCAATGTAGTTCGCCAAGTACAGACTTCTGGAGGAT
  TGTGGACTGAATGCATAGCTCAACTCAGTCCGGAGCAGCAGGCAGCCATCCAGGAGCTCCTGAACTCTGC
  CTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_023579
• Insert Size: 3294 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_023579.4, NP_076068.1
• RefSeq Size: 4598 bp
• RefSeq ORF: 3294 bp
• Locus ID: 70572
• UniProt ID: Q8BKC5
• Cytogenetics: 14 E4- E5
• Gene Summary: Functions in nuclear protein import as nuclear transport receptor. Serves as receptor for nuclear localization signals (NLS) in cargo substrates. Is thought to mediate docking of the importin/substrate complex to the nuclear pore complex (NPC) through binding to nucleoporin and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to the importin, the importin/substrate complex dissociates and importin is re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. Mediates the nuclear import of ribosomal proteins RPL23A, RPS7 and RPL5. Binds to a beta-like import receptor binding (BIB) domain of RPL23A. In vitro, mediates nuclear import of H2A, H2B, H3 and H4 histones. Binds to CPEB3 and mediates its nuclear import following neuronal stimulation (PubMed:22730302).[UniProtKB/Swiss-Prot Function]