Dlc1 (NM_015802) Mouse Untagged Clone

CAT#: MC223509

Dlc1 (untagged) - Mouse deleted in liver cancer 1 (Dlc1), transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Dlc1
• Synonyms: A730069N07Rik; Arhgap7; dlc-1; HP; STARD12
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC223509 representing NM_015802
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTGTAGAGACGAGCCGGACACCATGATCCTAACACAAATTGAAGCCAAGGAAGCTTGCGACTGGCTGA
  GAGTGACTGGTTTCCCCCAGTATGCACAGCTGTATGAAGATCTACTGTTCCCTATTGATATTGCGCTGGT
  CAAGAGAGAACATGACTTCTTGGACAGAGACGCCATCGAGGCTCTCTGCAGGCGCTTAAATACTCTAAAC
  AAGTGTGCAGTCATGAAGCTGGAGATCAGTCCTCACCGGAAGCGAAGTGAGGATTCGGATGAAGATGAGC
  CTTGCGCCATAAGCGGCAAGTGGACTTTCCAGAGGGACAGCAAGCGGTGGTCCCGCCTTGAAGAGTTTGA
  CGTCTTTTCCCCAAAGCAGGATCCAATCCCTGGGTCCCCAGACAACTCTCGTTTGCAAAGCGCCACAAGC
  CACGAAAGCATGCTGACAGACCTCAGCGAGCACCAGGAGGTGGCCTCTGTCCGAAGCCTCAGCAGCACCA
  GCAGCAGCGTCCCCACCCACGCAGCCCACAGTGGAGATGCCACTACGCCCCGAACCAATTCCGTCATCAG
  CGTCTGCTCCTCCGGACACTTTGTAGGCAATGATGACTCTTTTTCCAGCCTGCCGTCTCCCAAGGAACTG
  TCCAGCTTCAGTTTTAGCATGAAAGGCCACCACGAGAAGAACACCAAGTCGAAGACGCGGAGCCTGCTCA
  AACGCATGGAGAGCCTGAAGCTCAAGGGCTCCCACCACAGCAAGCACAAGGCGCCTTCCAAGCTGGGGTT
  GATCATCAGTGCTCCCATTCTGCAGGAGGGTATGGATGAGGCAAAGCTGAAGCAGCTGAACTGTGTGGAG
  ATCTCAGCCCTCAATGGCAACCACATCAACGTGCCCATGGTACGGAAAAGGAGCGTGTCTAACTCCACCC
  AGACCAGCAGCAGCAGCAGCCAATCAGAGACCAGCAGCGCGGTCAGCACACCCAGCCCGGTCACCAGGAC
  CCGGAGCCTCAGCACCTGTAACAAGCGGGTGGGCATGTATCTAGAGGGCTTCGACCCATTCAGTCAGTCC
  ACCTTGAACAACGTGACGGAGCAGAACTATAAAAACCGTGAGAGCTACCCAGAGGACACGGTGTTCTACA
  TTCCCGAAGATCACAAGCCCGGCACCTTCCCTAAGGCCCTCTCCCATGGCAGTTTCTGTCCCTCGGGAAA
  CAGTTCTGTGAACTGGAGGACCGGAAGCTTCCATGGCCCTGGCCATCTCAGCCTACGGAGAGAAAACAGC
  CATGACAGTCCTAAGGAGCTGAAGAGACGCAATTCTTCCAGCTCTCTGAGCAGCCGCCTGAGCATCTATG
  ATAACGTACCGGGTTCTATCCTGTACTCCAGCTCGGGAGAACTGGCCGACCTGGAGAATGAGGACATCTT
  CCCTGAGCTGGATGACATTCTCTACCACGTGAAGGGGATGCAGCGGATAGTCAACCAGTGGTCCGAGAAG
  TTTTCCGACGAGGGAGACTCGGACTCAGCCCTGGACTCTGTCTCTCCTTGCCCGTCATCTCCAAAACAGA
  TACACCTGGATGTGGACCATGACCGAAGGACACCCAGTGACCTGGACAGCACAGGCAACTCCCTCAATGA
  GCCCGAAGAGCCCACTGATATCCCGGAAAGAAGAGACTCAGGGGTGGGGGCTTCCCTGACCAGGTGCAAT
  AGGCACAGACTGAGGTGGCACAGCTTCCAGAGCTCCCACCGGCCGAGCCTCAACTCCGTGTCACTGCAGA
  TTAACTGCCAGTCTGTGGCCCAGATGAACCTGCTGCAGAAATACTCGCTCCTGAAATTGACGGCCCTGCT
  GGAAAAGTACACGCCTTCCAACAAGCACGGCTTCAGCTGGGCTGTGCCCAAGTTCATGAAAAGGATCAAG
  GTTCCAGACTACAAGGACCGGAGTGTGTTTGGGGTCCCACTGACTGTGAACGTGCAGCGCTCAGGACAGC
  CCCTGCCCCAGAGCATCCAGCAGGCCATGCGCTACCTCCGTAACCACTGTCTGGACCAGGTCGGGCTCTT
  CAGGAAGTCAGGTGTCAAATCCCGGATCCAGGCTCTACGCCAGATGAATGAAAGCGCTGAAGATAATGTC
  AACTATGAAGGCCAGTCTGCTTATGATGTGGCAGACATGTTAAAGCAATATTTTCGAGATCTTCCTGAGC
  CCCTCATGACGAACAAACTCTCCGAAACCTTCCTGCAGATCTACCAGTATGTTCCCAAGGACCAGCGCCT
  CCAAGCCATCAAGGCGGCCATTATGCTCCTGCCCGACGAGAACCGGGAGGTTCTGCAGACACTTCTCTAT
  TTCCTGAGCGATGTCACAGCGGCTGTGAAAGAAAACCAGATGACTCCCACCAACCTGGCTGTGTGCCTAG
  CTCCGTCCCTCTTCCACCTCAACACCCTGAAGCGAGAGAATTCTTCTCCAAGGGTAATGCAAAGAAAACA
  GAGTTTGGGCAAACCAGACCAGAAAGACCTGAATGAAAACCTAGCGGCGACTCAAGGGCTGGCCCACATG
  ATTGCTGAGTGCAAGAAGCTCTTCCAGGTCCCTGAGGAAATGAGCCGGTGCCGTAACTCCTACACTGAAC
  AAGAGCTGAAGCCCCTTACCCTGGAGGCCTTGGGACACCTGAATAGTGACCAGCCTGCTGACTACAGACA
  CTTCCTCCAGGACTGTGTGGATGGCCTGTTTAAGGAGGTCAAAGAGAAGTTCAAAGGCTGGGTCAGCTAC
  CCCACCTCCGAACAGGCTGAGCTGTCCTATAAGAAGGTCAGCGAAGGACCCCCGTTAAGGCTTTGGAGGT
  CAACTATCGAAGTCCCCGCTGCACCCGAGGAGATCTTAAAGCGCCTTCTGAAGGAGCAACACCTCTGGGA
  TGTGGACCTGCTGGACTCCAAGGTGATTGAAATCCTGGACAGCCAGACTGAAATCTACCAATACGTCCAA
  AACAGCATGGCGCCCCACCCTGCGCGGGACTACGTCGTATTGAGGACCTGGAGGACTAACTTACCCAGGG
  GGGCCTGTGCTCTCTTACTCACCTCTGTGGATCACGACCGGGCCCCCGTGGCGGGGGTGAGGGTTAATGT
  GCTCCTGTCCAGATATTTGATTGAACCCTGCGGGTCAGGAAAATCCAAGCTTACCTACATGTGCAGAGCT
  GATTTAAGGGGCCACATGCCGGAGTGGTACTCAAAATCTTTTGGACATTTGTGTGCAGCCGAAGTGGTAA
  AGATCCGAGACTCCTTCAGTAATCAGAACACTGAAAGCAAGGACACGAGATCTAGGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_015802
• Insert Size: 3279 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_015802.3, NP_056617.2
• RefSeq Size: 6184 bp
• RefSeq ORF: 3279 bp
• Locus ID: 50768
• UniProt ID: Q9R0Z9
• Cytogenetics: 8 23.05 cM
• Gene Summary: Functions as a GTPase-activating protein for the small GTPases RHOA, RHOB, RHOC and CDC42, terminating their downstream signaling. This induces morphological changes and detachment through cytoskeletal reorganization, playing a critical role in biological processes such as cell migration and proliferation. Also functions in vivo as an activator of the phospholipase PLCD1. Active DLC1 increases cell migration velocity but reduces directionality (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) differs in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at an alternate start codon, compared to variant 1. The encoded isoform (2) has a distinct N-terminus and is shorter than isoform 1.