Lifr (NM_013584) Mouse Untagged Clone

CAT#: MC223506

Lifr (untagged) - Mouse leukemia inhibitory factor receptor (Lifr), transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Lifr
• Synonyms: A230075M04Rik; AW061234; LIF
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC223506 representing NM_013584
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAGCTTACTCATGGTGGAGACAGCCATCGTGGATGGTAGACAATAAAAGATCGAGGATGACTCCAA
  ACCTGCCATGGCTCCTGTCAGCTCTGACCCTCCTGCATCTGACGATGCATGCAAACGGTCTGAAGAGAGG
  GGTACAAGACTTGAAATGCACAACCAACAACATGCGAGTGTGGGACTGCACGTGGCCAGCTCCCCTCGGG
  GTCAGCCCTGGAACTGTTAAAGATATTTGCATTAAAGACAGGTTCCATTCTTGTCACCCATTAGAGACAA
  CAAACGTTAAAATTCCAGCTCTTTCACCTGGTGATCACGAAGTCACAATAAATTATCTAAATGGCTTTCA
  GAGTAAATTCACGTTGAATGAAAAAGATGTCTCTTTAATTCCAGAGACTCCCGAGATCCTGGATTTGTCT
  GCTGACTTCTTCACCTCCTCCTTACTACTGAAGTGGAACGACAGAGGGTCTGCTCTGCCTCACCCCTCCA
  ATGCCACCTGGGAGATTAAGGTTCTACAGAATCCAAGGACGGAACCAGTAGCACTCGTGTTACTCAACAC
  AATGCTGAGTGGTAAAGATACCGTTCAGCACTGGAACTGGACCTCAGACCTGCCCTTGCAATGTGCCACT
  CACTCGGTGAGCATTCGATGGCACATTGACTCGCCTCATTTCTCCGGTTACAAAGAGTGGAGTGACTGGA
  GCCCGCTGAAGAACATCTCCTGGATTCGTAATACAGAGACTAATGTTTTTCCTCAAGACAAAGTGGTGCT
  CGCAGGCTCAAACATGACAATTTGTTGTATGAGTCCAACGAAAGTGCTTTCAGGACAGATCGGCAATACC
  CTTCGTCCTCTCATCCATCTGTACGGGCAAACCGTTGCGATCCATATCCTGAACATCCCCGTTTCTGAAA
  ACAGTGGCACAAACATCATTTTCATCACAGACGACGATGTGTACGGAACGGTGGTCTTTGCAGGCTATCC
  TCCCGATGTTCCTCAGAAGCTGAGCTGTGAGACACATGACTTAAAAGAGATTATATGTAGCTGGAATCCA
  GGAAGGATAACTGGACTGGTGGGCCCACGAAATACAGAATACACCCTGTTTGAAAGCATTTCAGGAAAAT
  CGGCAGTATTTCACAGGATTGAAGGACTTACAAACGAGACCTACCGGTTAGGCGTGCAAATGCATCCCGG
  CCAAGAAATCCATAACTTCACCCTGACTGGTCGCAATCCACTGGGGCAGGCACAGTCAGCAGTGGTCATC
  AATGTGACTGAGAGAGTTGCTCCTCATGATCCGACTTCGTTGAAAGTGAAGGACATCAATTCAACAGTTG
  TTACATTTTCTTGGTATTTACCAGGAAATTTTACAAAGATTAATCTTTTATGTCAAATTGAAATTTGTAA
  AGCTAATTCCAAGAAAGAAGTGAGGAATGCCACAATCAGAGGAGCCGAGGATTCAACTTACCATGTTGCT
  GTAGACAAATTAAATCCATACACTGCATACACTTTCCGGGTTCGTTGTTCTTCCAAGACTTTCTGGAAGT
  GGAGCAGGTGGAGTGATGAGAAGCGACATCTAACCACAGAAGCCACTCCTTCAAAGGGACCAGACACTTG
  GAGAGAGTGGAGTTCTGATGGAAAAAATCTAATCGTCTACTGGAAGCCTTTACCTATTAATGAAGCTAAT
  GGAAAAATACTTTCCTACAATGTTTCGTGTTCATTGAACGAGGAGACACAGTCAGTTTTGGAGATCTTCG
  ATCCTCAACACAGAGCAGAGATACAGCTTAGTAAAAATGACTACATCATCAGTGTGGTGGCAAGAAATTC
  TGCTGGCTCATCACCACCTTCGAAAATAGCTAGTATGGAAATCCCAAATGACGACATCACAGTAGAGCAA
  GCGGTGGGGCTAGGAAACAGGATCTTCCTCACCTGGCGTCACGACCCCAACATGACTTGTGACTACGTAA
  TTAAATGGTGCAACTCATCTCGGTCTGAGCCCTGCCTCCTGGACTGGAGAAAGGTTCCTTCAAACAGCAC
  GGAGACTGTCATAGAGTCTGATCAGTTTCAGCCAGGAGTAAGATACAACTTTTACCTCTATGGGTGCACT
  AACCAGGGATACCAACTGTTACGTTCCATAATTGGATACGTAGAAGAACTGGCTCCCATTGTCGCGCCAA
  ATTTCACCGTGGAAGATACGTCGGCAGACTCGATATTAGTGAAATGGGACGACATCCCCGTGGAAGAGCT
  CCGAGGCTTCCTAAGAGGGTATTTATTTTACTTTCAGAAAGGAGAGAGAGATACGCCCAAGACGAGGAGC
  TTGGAGCCACATCATTCTGACATCAAGCTAAAGAACATCACTGACATATCCCAGAAGACACTGAGGATTG
  CTGACCTTCAGGGTAAAACCAGTTACCATCTGGTCCTGCGAGCCTATACACATGGTGGTCTGGGCCCAGA
  GAAGAGCATGTTTGTGGTGACCAAGGAAAACTCTGTGGGATTGATTATTGCCATCCTCATCCCAGTGGCT
  GTGGCTGTCATTGTTGGCGTGGTAACGAGCATCCTTTGCTATCGGAAGCGAGAATGGATTAAGGAAACAT
  TCTACCCAGATATTCCCAATCCAGAAAACTGTAAGGCGCTACAGTTTCAGAAGAGCGTCTGCGAGGGAAG
  CAATGCTCTTAAGACATTGGAAATGAACCCCTGTACCCCGAACAACGTTGAAGTCCTGGAATCTCGGTCG
  ATAGTCCCTAAAATAGAAGATACAGAAATAATTTCCCCAGTTGCTGAGCGTCCTGGGGAAAGATCTGAGG
  TGGACCCTGAGAACCATGTGGTCGTGTCTTACTGCCCACCCATCATCGAGGAGGAAATAACGAACCCCGC
  GGCCGATGAAGTGGGAGGGGCTTCCCAGGTCGTGTACATCGATGTGCAGTCCATGTATCAGCCGCAAGCC
  AAAGCAGAGGAAGAGCAGGACGTGGATCCTGTGGTGGTGGCAGGCTATAAGCCACAGATGCGCCTTCCCA
  TCAGCCCCGCTGTGGAAGACACAGCAGCAGAAGATGAAGAGGGTAAGACCGCCGGTTACAGACCTCAGGC
  CAATGTAAACACTTGGAATTTGGTCTCTCCAGATTCCCCGAGGTCCACAGACAGCAACAACGAAGTTGTC
  TCTTTTGGAAGTCCGTGCTCCATCAATTCCAGGCAATTCTTGATTCCTCCTAAAGACGAAGACTCTCCTA
  AATCTAATGGAGGAGGGTGGTCCTTTACAAACTTCTTCCAGAACAAACCAAATGACTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_013584
• Insert Size: 3279 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_013584.2, NP_038612.1
• RefSeq Size: 9663 bp
• RefSeq ORF: 3279 bp
• Locus ID: 16880
• UniProt ID: P42703
• Cytogenetics: 15 3.46 cM
• Gene Summary: Signal-transducing molecule. May have a common pathway with IL6ST. The soluble form inhibits the biological activity of LIF by blocking its binding to receptors on target cells.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) encodes the longer isoform (1). Both variants 1 and 3 encode the same protein. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.