Ipo4 (NM_024267) Mouse Untagged Clone

CAT#: MC223483

Ipo4 (untagged) - Mouse importin 4 (Ipo4), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Ipo4
• Synonyms: 8430408O15Rik; AA409693; Imp4a; RanBP4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC223483 representing NM_024267
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGCCTGCGGGTTTGGAACAGATCCTGAAGGAGCTGCTGCTGCCCGACACGGAGCGCATCCGCCGGG
  CTACGGAGCAGCTCCAGACCATCCTTCGGGACCCTGCTGCCTTGCCCGCACTCTTCGATCTGCTGGCAAC
  AGCGACCGACTCTCAGATCCGCCAGTTTGCAGCGGTCCTGACCCGCAGAAGGCTGAACAATCGCTGGCGA
  CGCCTGGCACCTGAGCAGAGGGAGAGCCTCAAGTCCCTGGTCCTGACAGCACTGCAGAAAGAGACAGTGC
  ATTCTGTGAGTGTCAGCTTGGCCCAGCTCTCAGCCACCATTTTTCGAAAGGAAGGCCTACAAGGATGGCC
  TCAGTTCATGAACCTTCTTCAGCACAGCACTCATAGTTCCCATAGCCCCGAGAAAGAGGTGGGACTTCTG
  CTGCTGAGTGTGGTGGTGTCTTCCCAGCCCGAGGCATTCCATGCCCACCAGCACGAGCTGCTTCAGCTTC
  TGAATGAGACGCTCAGTGACGTCAGCTTTCCCGGAGTGCTCTTCTACTCCCTGCGCACTCTGACTGCCAT
  CGCCCGCTATGTCAGGCCGGATGATGTGTCTCTTGCACGGATGTTGGTGCCCAAAGTCGTTACGGCACTG
  AGGACTCTGATCCCCTTAGATGAGGTAAAGGCCTGTGAGGCCCTGGAGGCCCTGGATGAAATGCTGGAGA
  CAGAGTTGCCCATCATCAACCCCCACCTCTCTGAGGTCCTCACGTTTTGCCTGGAGGTGGCTAAGAATGT
  GGCCCTTGGCGAACCACTCCGCGTACGTGTTCTCTGCTGCCTCACTTTCTTGGTCAAAGTCAAAAGTAAG
  GCCTTACTAAAGAACCGCCTCGTGCCTCCCTTGCTGCACGCCCTTTTCCCCCTCATGGCTGCTGAGCCAC
  CCATGGGCCAACTGGATCCTGAAGATCAAGATTCGGATGACGATGACTTGGAGATCGGGCTGATGGGGGA
  GACTCCCAAGCACTTTGCTGTACAGGTTGTGGACATGCTGGCACTACATCTTCCCCCTGAGAAGCTCTGT
  CCCCATGTGATGCCCATGCTGGAAGAAGCCTTGCGGAGCGAGGACCCATACCAGCGCAAAGCAGGATTCC
  TGGTGTTGGCTGTGCTGTCTGATGGTGCAGGCGACCACATCAGGCAGAGACTGCTGTACCCACTGCTACA
  GATTGTGTGCAAGGGCCTGGATGACCCCTCACAGATTGTTCGTAATGCTGCTCTGTTCGCCCTGGGCCAG
  TTTTCAGAGAACTTACAGCCCCATATCAGCAGCTACTCTGAGGAGGTGATGCCCCTTCTCCTCTCCTACC
  TGAAGTCAGTGCCGATGGGAAACACACACCACCTGGCCAAGGCCTGCTATGCTCTGGAGAATTTTGTGGA
  GAACCTAGGGCCCAAAGTGCAGCCCTACCTTCCGGAGCTTATGGAATGTATGCTGCAGCCATTGAAGAAC
  CCCAGCAAAGCCCGGACCAAGGAGCTAGCTGTGAGCGCCATAGGGGCCATTGCCACAGCGGCCCAGGATT
  CCCTGCTGCCCTACTTCCCTACCATCATGGATCTTCTCCGGGAGTTCTTGTTGACAGGCCACGAAGACTT
  TCATCTTGTGCAGATCCAGAGCCTGGAGACCCTGGGAGTGCTAGCACGAGCCCTGGGAGAGTCCATGAAG
  CCCCTGGCTGAGGAATGCTGTCAGCTTGGGCTAGGGTTATGCATCCATATAGATGACCCTGACGTGAGGC
  GCTGCACGTACAGCCTGTTTGCAGCCTTATCAGGGTTAATGGGAGAGGGCTTGGGACCCTACCTGCCTCA
  GATTACCACGCTCATGCTGCTGTCGCTGCGCTCCACTGAGGGCATTGTGCCACAGTATGACGGGATCAGC
  TCCTTCCTGCTGTTTGATGACGACAGCGAAGCAGAAGAGGAGGAGGAGCTCATGGATGAAGATATGGAAG
  AGGAGGGGGATGACTCTGAGATCTCGGGGTATAGTGTGGAGAATGCCTTCTTTGATGAGAAAGAAGACAC
  CTGCACCGCCCTGGGGGAGATCTCCATGAACACCTGTGTGGCTTTTCTCCCATTCATGGATGCCACCTTC
  GATGAAGTATACAAACTGCTGGAGTGCCCTCATATGAATGTGCGGAAGTCAGCTTACGAAGCGCTGGGAC
  AGTTCTGCTGTGCACTGCATAAGGCCTCTCAGAGAAGCTCCTCAGATCCCAGCAGCAGTCCCGTTCTGCA
  GACCTCCCTGGCTCGAGTGATGCCAGCTTACATGCAGGCCGTGAAAGTAGAAAGGGAGCGCCCAGTGGTG
  ATGGCCGTGCTGGAGTCCCTGACAGGGGTGCTGCGCACCTGCGGCAGCCTTGCACTGCAGCCTCCTGGGC
  GGCTCAGTGAGCTCTGCAACGTGCTCAAGGCTGTGCTGCAGAAAAAGACGGCCTGTCAGGACGCCGAGGA
  GGATGATGACGAAGATGATGACCAGGCAGAGTATGACGCCATGTTACTAGAACATGCTGGAGAGGCCATT
  CCCGTCCTGGCAGCCACAGCTGGAGGACACGCCTTCGCTCCCTTCTTTGCCACGTTCTTGCCATTGTTGC
  TGTGTAAGACGAAACAGAGCTGTACAGTGGCAGAGAAGTCCTTTGCTGTGGGAACCCTGGCAGAGTCCAT
  TCAGGGTCTAGGGACCGCCTCAGCCCAGTTTGTGTCTCGGCTGTTCCCTGTGCTGTTAAACAATGCCCGG
  GAAGCAGACCCTGAGGTGCGGAGCAATGCCATCTTTGGGTTGGGCGTACTGGCAGAGCATGGCGGCTGCC
  CTGCTCAGGACCACTTCCCTAAGCTACTGGGCCTCCTTTTGCCCCTGCTGGCAAGGGAGCGACATGATCG
  AGTCCGTGATAACATCTGCGGGGCTCTTGCCCGTGTACTGATGGCCAGTCCAGTAGGAAAAACGGAGCCC
  CAGGTGCTGGCTACCCTGCTACGTGCCCTGCCCCTGAAGGAAGACATGGAGGAGTGGCTCACTATAGGTC
  ACCTCTTCAGCTTCCTGCACCAGAACAATCCTGAGCAGGTTGTGGATGTGGCTTCAGAACTCCTGCGCAT
  CTGCAGCCTGATCCTGCCGGACAACCGGATCCCTCCAGACACCAAGGCGGCCCTGCTGCTGCTCCTGACG
  TTCCTGGCCAAGCAGCACACCGACAGCTTCCACACAGCCCTGGGCTCTCTGCCAAATGATAAAGCTCAGG
  AACTTCAGGCCATGATGGGCCTCACTTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_024267
• Insert Size: 3249 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_024267.6, NP_077229.4
• RefSeq Size: 3611 bp
• RefSeq ORF: 3249 bp
• Locus ID: 75751
• UniProt ID: Q8VI75
• Cytogenetics: 14 28.19 cM
• Gene Summary: Functions in nuclear protein import as nuclear transport receptor. Serves as receptor for nuclear localization signals (NLS) in cargo substrates. Is thought to mediate docking of the importin/substrate complex to the nuclear pore complex (NPC) through binding to nucleoporin and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to the importin, the importin/substrate complex dissociates and importin is re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus (By similarity). Mediates the nuclear import of RPS3A. Acts as chaperone for exposed basic domains.[UniProtKB/Swiss-Prot Function]