Kdm4a (NM_001161823) Mouse Untagged Clone

CAT#: MC223429

Kdm4a (untagged) - Mouse lysine (K)-specific demethylase 4A (Kdm4a), transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Kdm4a
• Synonyms: D4Ertd222e; JHDM3A; Jmjd2; Jmjd2a; mKIAA0677
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC223429 representing NM_001161823
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTTCTGAATCAGAAACTTTGAACCCCAGTGCTCGGATCATGACCTTTTACCCCACCATGGAAGAGT
  TCCGGAACTTCAGCCGCTACATTGCCTACATTGAATCCCAAGGAGCTCATCGGGCTGGGCTAGCCAAGGT
  CGTTCCTCCCAAAGAGTGGAAGCCTCGAACATCCTACGACGATATTGATGATCTAGTCATCCCTGCGCCC
  ATCCAGCAGCTGGTGACTGGCCAGTCTGGCCTCTTCACTCAGTACAACATACAGAAGAAAGCCATGACCG
  TTCGTGAGTTCCGCAAGATCGCCAATAGCGACAAGTACTGCACCCCGCGATACAGTGAATTTGAAGAACT
  TGAGCGTAAATACTGGAAAAACCTCACATTCAATCCTCCCATCTATGGAGCAGACGTGAATGGTACTCTC
  TATGAACAGCACGTTGATGAGTGGAATATCGGCCGGCTGAAGACCATCCTGGACCTGGTGGAGAAGGAGA
  GCGGGATCACCATTGAGGGGGTGAACACCCCCTACCTTTACTTCGGCATGTGGAAGACGTCCTTCGCCTG
  GCACACGGAAGACATGGATCTATACAGCATCAACTATCTGCACTTCGGAGAGCCAAAGTCTTGGTACTCT
  GTTCCACCTGAGCATGGGAAACGCCTGGAGCGTCTTGCCAAAGGCTTTTTCCCAGGAAGTGCTCAAAGCT
  GTGAGGCTTTTCTCCGCCACAAGATGACCCTGATCTCCCCATTAATGCTGAAGAAGTATGGCATTCCCTT
  TGACAAGGTGACCCAAGAAGCTGGCGAGTTTATGATCACTTTTCCGTATGGTTACCATGCTGGCTTCAAC
  CATGGCTTCAACTGTGCAGAGTCAACCAATTTTGCTACCCGTCGGTGGATTGAGTATGGCAAGCAGGCTG
  TGCTGTGCTCCTGCAGAAAAGATATGGTGAAGATCTCCATGGACGTGTTCGTGAGGAGGTTCCAGCCGGA
  ACGCTACAAACTTTGGAAAGCTGGGAAGGACAGCATGGTTATTGACCACACTCTGCCCACACCGGAAGCA
  GCCGAGTTCCTGAAGGACAGCGGCGGACTAACCCCGAGAGCAGGGAGTGAGGAGTGCCCAGAGGAGGACG
  TGGAAGCAGCGGATCAGGGAGAGGAGGGGGATGTGAAGAGAAGCCTGGCCAAGCATCGGATCGGGACAAA
  GAGACACAGAGTCTGTCTGGAAATACCCCAGGAGGTCAGTCAGAGCGAGCTCTTCCCCAAGGAAGAGCTA
  AGTTCTGGACAGTATGAGATGACAGAGTGCCCGGCCACCCTCGCCCCAGTGAGGCCCACCCACAGCTCCG
  TGCGGCAAGTTGAGGACAGTCTTCCCTTCCCAGATTACTCTGACCCCACTGAAGTCAAATTTGAAGAGCT
  GAAGAATGTCAAACTAGAAGAGGAAGATGAGGAGGATGAGCCCGAAGCAGCCGCACTGGACCTTTCTGTG
  AATCCTGCGTCTGTAGGAGGACGCCTCGTCTTCTCGGGTTCCAAAAAGAAATCATCTTCCAGCCTGGGCT
  CCACTTCATCTCAGGATTCAGTTTCTTCAGATTCTGAAACCGCCGAGTCTGTCTCTTGCCAGGGCCAAGA
  GAAAACGGGAGTTCTCACCGTGCACAGCTATGCCAGAGGGGACGGCAAGGCTGCCACAGGAGAGCCGAGT
  GTGAAGAAGAAGCGCAGTGCTCCCCGCAGCATCAGCGAGCAGGAGCTGGCAGAGGTTGCAGATGAGTACA
  TGCTTTCCCTGGAGGAGAATAAGAAGACCAAGGGTCGCCGCCAGCCCTTGAGCAAGCTCCCACGCCACCA
  CCCACTCGTGCTGCAGGAGTGCGGCAGCGATGACGAGACTTCTGAGCAGCTGACCCCCGAGGAGGAGGCT
  GAGGAGACAGAGGCTTGGGCCAAGCCTCTGAGCCAGCTGTGGCAGAACCGACCCCCGAACTTTGAGGCTG
  AAAAGGAATTCAATGAGATCATGGCCCAACAGGCCCCTCACTGTGCTGTGTGTATGATCTTCCAGACCTA
  CCATCAGGTGGAATTTGGAGCCTTCAGTCAGAGCTGTGGGGATGCTTCAGAGCCGGCTGCCCAGACTCAG
  AGGACCAAGCCACTGATCCCCGAAATGTGCTTTACCACCACTGGCTGCAGCACAGACATCAATCTGTCCA
  CCCCGTACCTAGAGGAGGATGGCACCAGCATGCTAGTGTCCTGCAAGAAGTGCAGTGTCCGGGTCCACGC
  CAGTTGCTATGGCGTCCCTCCTGCCAAGGCTTCTGAAGAATGGATGTGCTCTCGATGTTCAGCCAATGCC
  CTGGAGGAAGACTGCTGCTTATGCTCGTTGCGTGGAGGGGCCCTGCAGAGAGCCAATGACGACAGATGGG
  TTCATGTTTCATGTGCAGTGGCAATTCTAGAAGCAAGGTTTGTCAACATTGCTGAAAGAAGTCCAGTGGA
  TGTGAGCAAAATCCCTCTGCCCCGCTTCAAACTGAAGTGTGTTTTTTGTAAGAAGCGGAGAAAACGGAAT
  GCTGGCTGCTGTGTGCAGTGCTCCCATGGCCGCTGCCCCACTGCCTTCCATGTGAGCTGTGCCCAGGCTG
  CTGGGGTGATGATGCAACCCGACGACTGGCCCTTTGTTGTCTTCATCACGTGCTTCCGTCACAAGATTCC
  CAATCTGGAGCGTGCTAAGGGAGCCTTGCTGAGCATCACGGCGGGCCAGAAGGTTATCAGCAAACACAAG
  AACGGGCGCTTCTACCAGTGTGAGGTGGTCAGACTCACCACCGAGACCTTCTACGAAGTCAACTTTGACG
  ACGGCTCCTTCAGTGACAACCTTTATCCTGAGGACATAGTGAGTCAGGACTGTCTCCAACTGGGGCCTCC
  TGCTGAAGGTGAAGTGGTTCAAGTCAGATGGACAGATGGCCAAGTCTATGGGGCTAAGTTTGTGGCCTCC
  CACCCCATCCAGATGTACCAGGTGGAGTTTGAGGATGGCTCGCAGCTGGTGGTTAAGAGAGATGATGTAT
  ACACACTCGATGAAGAGCTTCCCAAGAGAGTCAAGTCTAGACTGTCGGTGGCTTCAGACATGCGCTTCAA
  TGAGATTTTCACAGAGAAGGAAGTGAAGCAAGAAAAGAAGAGGCAACGGGTCATCAACTCGCGGTACCGG
  GAGGATTACATCGAGCCAGCGCTGTACCGGGCCATCATGGAGTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001161823
• Insert Size: 3195 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001161823.1, NP_001155295.1
• RefSeq Size: 4616 bp
• RefSeq ORF: 3195 bp
• Locus ID: 230674
• UniProt ID: Q8BW72
• Cytogenetics: 4 54.31 cM
• Gene Summary: Histone demethylase that specifically demethylates 'Lys-9' and 'Lys-36' residues of histone H3, thereby playing a central role in histone code (PubMed:24953653). Does not demethylate histone H3 'Lys-4', H3 'Lys-27' nor H4 'Lys-20'. Demethylates trimethylated H3 'Lys-9' and H3 'Lys-36' residue, while it has no activity on mono- and dimethylated residues. Demethylation of Lys residue generates formaldehyde and succinate. Participates in transcriptional repression of ASCL2 and E2F-responsive promoters via the recruitment of histone deacetylases and NCOR1, respectively (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.