Usp53 (NM_133857) Mouse Untagged Clone

CAT#: MC223425

Usp53 (untagged) - Mouse ubiquitin specific peptidase 53 (Usp53), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Usp53
• Synonyms: AA939927; mbo; mKIAA1350; Phxr3; Sp6
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC223425 representing NM_133857
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCTGGGTAAAATTTTTACGGAAGCCTAGCGGCAATCTTGGCAAAGCTTATCAAGCTGGAAGCCTGC
  TGTCCCTAGCCCCTACTGTGGGCTTGTTAAACGAGCCAGGGCAGAACAGCTGCTTTCTAAACAGTGCTGT
  ACAGGTTTTATGGCAATTAGATATATTCCGAAGAAGCTTGAGAGCTCTGACTGGACACATATGTCAGGGA
  GATGCCTGTATATTTTGTGCATTGAAGACAATATTTGCACAATTTCAACACAGTCGAGAAAAAGCACTTC
  CCTCAGATAACATAAGGCATGCTCTTGCAGAAAGCTTCAAAGATGAGCAGAGATTTCAGCTTGGCCTCAT
  GGATGATGCTGCCGAGTGCTTTGAAAATATATTGGCAAGAATTCATTTTCACCTGGTGCCAAACAGGGAC
  GCAGACATGTGCACCTCTAAGTCTTGTGTGACTCATCAGAAGTTTGCCATGACTCTGTATGAGCAGTGTG
  TGTGCCGGAGCTGTGGCGCTTCCTCAGACCCTCTGCCCTTTACCGAACTCGTGAGGTACATTTCTACAAC
  AGCCCTGTGCAATGAAGTTGAAAGAATGATGGAAAGGCATGAGCGAGTTAAACCAGAGATGTTTGCGGAA
  CTGTTGCAAGCTGCAAACACAGCAGATGACTATCGGAAGTGTCCTAGTAACTGTGGCCAAAAAATAAAAA
  TCCGCCGTGTTTTAATGAATTGCCCAGAGATTGTTACAATTGGATTAGTGTGGGACTCCGAGCACTCAGA
  CTTGACTGAAGATGTTGTTCGAAGTCTAGCCACGCATCTCTACCTTCCTGGGCTTTTTTATAGGGTCACT
  GATGAGAATGCCACAGACAGCGAGCTGCACCTTGTGGGTATGATCTGTTACACCAGCCGACACTACTGTG
  CCTTTGCTTTTCACACCAAGAGCTCCAAGTGGGTGTTTTTTGATGATGCACACGTGAAGGAGATGGGAAC
  CAGATGGAAAGATGTGGTCTCCAAGTGCATACGATGCCACTTACAGCCCCTTTTGCTGTTTTATGCAAAC
  CCGGATGGTACAGCGGTCTCTACTGAGGATGCACTCAGGCAGGTTGTCCACTGGTCACATTACAGATCGG
  GTGAAGAAAACATGGGATGTGGAAAGCCTATCATTTATAAACCAGATAATTCAAAGGAAAATGGATTTGG
  TGGTCAGACGAAACAGAAAGAAAATCACAAATTTCAAACTGATATTTCCTCACTTAATCGGAGCCAAATG
  CAGACAAGTGGGAGGAGAGCACCAGTTAAGTTAAGCCATGATCAAAGAGAAAAGATAAAAGACATTTCCA
  GAGAATGTGCTCTGAAAGCCATTGAACAGAAAAATGCACTTTCTTCTCAACGGAAAGATTTAGAAAAGGG
  ACAGAGGAAAGATACAGGCCGACACAGGGATTTGGTTGATGAAGTCTTGGCATCTTTCAAGTCCGGATCA
  CCTCCTGCCTCGGATGGCTTTAGACAGCAAGGGAATCCCCATCTCTACCACAGCCAAGGCAAAGGACCTT
  GTAAACATGACCGAGCCACCCATGAGAGCTACACCTCTGGAAAAGTGATAAGTTCAAGCAAATCCCAGAA
  TCTTCTTGTTCCTGGAGAGAAAACAACCGGCAAAGCTAAGAGTGACAGTGGCACTGGATATGAAACAGAC
  AGCAGCCAAGATTCAAGGGACAAAGGAGGCAGCTACAGTAGTAAGACTAAAAGCCGCCACCGTGGCTGGA
  AACCCATGAGAGAAACCCTAAACGTCGATAGCGTTTTTAGTGAGAGCGAGAAGAGGCAGCACAGTCCGAG
  GCGGAAGTCGGATATCGGTAGCAGGCCTAGGTGTAGCAAGGACCAGAGTTTTAATAACTGGCCAAAGGCA
  AACCCAAAGCAAAAAGGCTTAATGACCATATACGAAGATGAAATGAAGCAAGAGGCAGGAAGCAGAAGTT
  CCCTTGAAACTAATGGAAAAGGAGCAGAGAAAAATCTAAGTGCTGCAGAGAGCAGAGGTCCTGGTACCAG
  CTGGCAGATGCAAAGGACAGAGTCTGGGTATGAGAGCAGCGATCATGTCAGTAACGGATCTGCCAGCTTG
  GACTCTCCTGGTGTGGAGGGAAGTGGGGCAGTCATGGATGTCGGTGGTGGGAAAGCCTTCAGTGAACATA
  TTAAGATGAACAGCCATAATATGGACAGTATGGAGTATATATCTCATCATTGCAAAGACCACCCAGAAGG
  CTTTAGGAAAGAACTCCAGGACTTGGAAGCAGATGATAAAATTCATGAGCTCCACCCAGAATCACATGTG
  CAAATAAAAAGCCATTTGATAAAAAGGTCACAGATTGGTGAAACCAATGACAAGTTGTTTCCTTCAGCCA
  GCCCACAAACACTTGCAAGAGAGCATGTTTACCAGACAAATGAACACAAAGTAGAAAGACCAGATAGGAG
  CAAATGTTCAGAGAGGCATAATACAGAAAACTCTGAGGGAACAGGCTTGCCATTTCACGTTGATGAGAGT
  TCTGTGGCTGGGAAGAGAGTCGACAGTAACGAAACAGTCTCGCCATCTTCACTGCCTTCATCTGTGAGAA
  CTGCTGGGCTAAAGCCCGAAACTGGACCCCTCATGTTTTGGTCACAGCAAAATATCACAGAACAGGGTTA
  CTCTGACAACTCTCTGTCCAGAGAACTTACACTTCTGTCTGCCTGCAATGCTGATAGTTGTCAGATGCCA
  AAACTCCACTGCCACCGTTCACCACCCCCTTTGCCACCAAAGAAATACTCTACAGCCAGTGCACCAAGGT
  TAGAGAGAGTTGGACTTAGCCCTGATGTCGGGGTTACCGAGGCGTTTAACACTAATCCCTCCAGCCTTCC
  AAAACACAGCTTGAGTCCAGCCTCAGGACCAAGTTTAGAAGGGAGTCCATGCATGACCCAGGAAAGAGAT
  AAAGAAACTATTCAAGTGAAGCAACTTGCTGCCAACAGCTACCCGTCCAGTTGTTCAACTAACAGTTTTC
  AGCCAGATCAAGACTCTACTTCTGTGTGTCCAAATGAAACAATTTCATTAACTACCTATTTCTCAGTTGA
  TAGTTGCATGACTGATACCTACAGATTGAAATACCATCAAAGACCTAAGCTCTATTTTCCAGAGAGCAGT
  GGCCATCATAGTAATAATTCACTCTCTCAAACTGAGCAAGTAGAGGGATCCATTACATAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_133857
• Insert Size: 3210 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_133857.3, NP_598618.1
• RefSeq Size: 4014 bp
• RefSeq ORF: 3210 bp
• Locus ID: 99526
• UniProt ID: P15975
• Cytogenetics: 3 G1
• Gene Summary: Tight junction-associated protein that is involved in the survival of auditory hair cells and hearing. Maybe by modulating the barrier properties and mechanical stability of tight junctions (PubMed:26609154). Has no peptidase activity (PubMed:26609154).[UniProtKB/Swiss-Prot Function]