Pik3cb (NM_029094) Mouse Untagged Clone

CAT#: MC223419

Pik3cb (untagged) - Mouse phosphatidylinositol 3-kinase, catalytic, beta polypeptide (Pik3cb), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Pik3cb
• Synonyms: 1110001J02Rik; AI447572; p110beta
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC223419 representing NM_029094
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCTCCTGCTATGGCAGACAACCTTGACATCTGGGCAGTGGACTCACAGATTGCATCCGATGGCGCCA
  TATCCGTCGATTTCCTTCTGCCCACCGGGATTTATATCCAGTTGGAAGTACCTCGGGAAGCTACCATTTC
  TTATATTAAACAGATGTTATGGAAGCAAGTTCACAACTACCCGATGTTTAACCTCCTCATGGACATTGAC
  TCGTATATGTTTGCATGTGTGAATCAAACTGCTGTATATGAGGAACTGGAAGACGAAACACGAAGACTTT
  GTGATGTCAGACCTTTTCTTCCAGTTCTCAAACTAGTGACTAGAAGCTGTGACCCCGCAGAAAAATTGGA
  CTCAAAAATTGGGGTTCTTATAGGAAAAGGTCTTCATGAGTTTGATGCCTTGAAGGATCCCGAAGTGAAT
  GAATTTAGAAGAAAAATGCGCAAATTCAGTGAGGCCAAGATTCAGTCTCTGGTAGGGTTGTCTTGGATCG
  ACTGGCTAAAGCACACGTATCCGCCTGAGCACGAGCCGTCCGTCCTGGAGAACTTGGAAGATAAACTTTA
  TGGAGGAAAGCTGGTTGTGGCTGTGCACTTTGAAAATAGCCAGGATGTATTTAGTTTTCAAGTGTCTCCC
  AATTTGAATCCTATAAAAATAAATGAATTGGCAATCCAGAAACGCCTCACTATTCGTGGAAAGGAAGATG
  AAGCTAGCCCCTGTGACTATGTGTTACAGGTCAGTGGGAGAGTGGAGTATGTGTTTGGCGATCATCCACT
  AATTCAGTTCCAGTACATCCGGAATTGTGTGATGAATAGAACCCTGCCCCACTTCATCCTTGTGGAATGT
  TGTAAGATCAAGAAAATGTATGAACAAGAAATGATTGCCATAGAGGCTGCCATCAACCGAAACTCATCCA
  ACCTTCCTCTCCCTTTACCACCAAAGAAAACGCGAGTTATTTCTCATATCTGGGACAACAACAACCCTTT
  CCAAATTACCTTGGTTAAAGGAAATAAGCTTAATACAGAAGAAACTGTGAAAGTTCATGTCCGAGCTGGG
  CTTTTTCACGGAACCGAGCTCCTGTGTAAAACCGTCGTAAGCTCAGAGATATCAGGAAAGAACGACCATA
  TTTGGAATGAACAACTGGAATTTGATATTAATATTTGTGACTTACCAAGAATGGCTCGATTATGTTTTGC
  TGTTTATGCAGTTTTGGATAAAGTAAAAACGAAGAAATCAACAAAGACTATTAATCCCTCTAAGTATCAG
  ACCATCAGGAAAGCCGGGAAAGTGCATTATCCTGTCGCATGGGTAAATACCATGGTTTTTGACTTCAAAG
  GACAGCTGAGGTCTGGAGACGTCATATTGCATAGCTGGTCTTCGTTTCCTGATGAGCTGGAAGAAATGCT
  GAATCCCATGGGGACTGTGCAGACGAACCCATATGCTGAGAACGCCACCGCCTTGCACATTACGTTCCCA
  GAGAATAAGAAGCAGCCGTGTTATTATCCCCCCTTCGATAAGATCATTGAGAAGGCAGCTGAGCTTGCCA
  GCGGAGACAGTGCTAATGTGTCAAGTCGTGGTGGAAAAAAATTTCTTGCTGTGCTGAAAGAAATCTTGGA
  CAGGGACCCCCTGTCTCAGCTGTGTGAGAACGAAATGGACCTTATTTGGACTCTACGGCAAGACTGCCGA
  GAAAATTTCCCTCAGTCACTGCCAAAACTACTCTTGTCAATCAAGTGGAATAAACTTGAAGATGTTGCTC
  AGCTTCAGGCGCTCCTGCAGATATGGCCCAAACTGCCCCCCAGGGAAGCCCTGGAACTCCTGGATTTCAA
  CTATCCAGACCAGTATGTCCGGGAATACGCTGTAGGCTGCCTTCGACAGATGAGTGATGAAGAACTCTCT
  CAGTATCTTTTACAATTGGTGCAAGTTTTGAAATATGAGCCTTTTCTCGATTGTGCCCTCTCCAGATTCC
  TATTAGAAAGAGCACTTGATAATCGGAGGATTGGGCAGTTTCTGTTTTGGCATCTTAGGTCAGAGGTGCA
  CACTCCTGCTGTGTCCGTACAGTTTGGTGTCATCCTGGAAGCATACTGTCGAGGAAGCGTGGGGCACATG
  AAAGTGCTTTCCAAACAGGTGGAAGCACTCAATAAGTTAAAAACTTTAAATAGCTTAATCAAACTGAATG
  CGGTGAAGCTGAGCAGAGCTAAGGGAAAGGAGGCCATGCACACGTGCCTGAAACAGAGTGCTTACCGGGA
  GGCGCTCTCTGACCTGCAGTCGCCGCTGAACCCCTGCGTCATCCTCTCAGAGCTCTATGTTGAAAAGTGC
  AAATACATGGACTCCAAGATGAAGCCCCTGTGGCTGGTCTACAGCAGCAGAGCCTTTGGAGAGGACTCGG
  TTGGAGTGATCTTTAAAAATGGTGACGATTTGCGGCAGGACATGCTGACGCTGCAGATGTTGCGCCTGAT
  GGATCTGCTTTGGAAAGAAGCTGGCTTGGACCTGCGGATGCTCCCCTATGGCTGCTTAGCAACAGGAGAT
  CGCTCTGGCCTCATTGAGGTTGTGAGCACCTCTGAGACAATCGCTGACATTCAGCTGAACAGTAGTAACG
  TGGCTGCCACGGCAGCCTTCAACAAAGACGCACTCCTGAACTGGCTCAAGGAGTACAACTCTGGGGATGA
  CCTGGACCGAGCGATTGAGGAGTTTACCTTGTCCTGTGCTGGCTACTGTGTAGCCTCTTATGTCCTCGGC
  ATTGGTGACAGGCACAGTGACAACATCATGGTGAAGAAAACCGGCCAGCTCTTCCACATAGATTTTGGGC
  ATATTCTTGGAAATTTCAAATCTAAATTTGGCATTAAAAGGGAGCGAGTACCTTTTATTCTTACTTATGA
  CTTCATTCATGTCATTCAACAAGGAAAAACGGGAAACACTGAAAAATTTGGCAGATTCCGCCAGTGCTGT
  GAAGATGCGTATCTGATTTTACGGCGGCATGGGAATCTCTTCATCACCCTGTTTGCCCTGATGTTGACTG
  CAGGGCTGCCTGAGCTCACATCGGTCAAAGATATACAGTATCTTAAGGACTCGCTTGCCTTAGGGAAGAG
  CGAGGAGGAAGCACTGAAGCAGTTCAAGCAGAAGTTTGACGAGGCCCTCAGGGAAAGCTGGACTACTAAA
  GTGAACTGGATGGCTCACACAGTACGGAAAGACTACAGGTCCTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_029094
• Insert Size: 3195 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_029094.3, NP_083370.2
• RefSeq Size: 4854 bp
• RefSeq ORF: 3195 bp
• Locus ID: 74769
• UniProt ID: Q8BTI9
• Cytogenetics: 9 E3.3
• Gene Summary: Phosphoinositide-3-kinase (PI3K) that phosphorylates PtdIns (Phosphatidylinositol), PtdIns4P (Phosphatidylinositol 4-phosphate) and PtdIns(4,5)P2 (Phosphatidylinositol 4,5-bisphosphate) to generate phosphatidylinositol 3,4,5-trisphosphate (PIP3). PIP3 plays a key role by recruiting PH domain-containing proteins to the membrane, including AKT1 and PDPK1, activating signaling cascades involved in cell growth, survival, proliferation, motility and morphology. Involved in the activation of AKT1 upon stimulation by G-protein coupled receptors (GPCRs) ligands such as CXCL12, sphingosine 1-phosphate, and lysophosphatidic acid. May also act downstream receptor tyrosine kinases. Required in different signaling pathways for stable platelet adhesion and aggregation. Plays a role in platelet activation signaling triggered by GPCRs, alpha-IIb/beta-3 integrins (ITGA2B/ ITGB3) and ITAM (immunoreceptor tyrosine-based activation motif)-bearing receptors such as GP6. Regulates the strength of adhesion of ITGA2B/ ITGB3 activated receptors necessary for the cellular transmission of contractile forces. Required for platelet aggregation induced by F2 (thrombin) and thromboxane A2 (TXA2). Has a role in cell survival. May have a role in cell migration. Involved in the early stage of autophagosome formation. Modulates the intracellular level of PtdIns3P (Phosphatidylinositol 3-phosphate) and activates PIK3C3 kinase activity. May act as a scaffold, independently of its lipid kinase activity to positively regulate autophagy. May have a role in insulin signaling as scaffolding protein in which the lipid kinase activity is not required. May have a kinase-independent function in regulating cell proliferation and in clathrin-mediated endocytosis. Mediator of oncogenic signal in cell lines lacking PTEN. The lipid kinase activity is necessary for its role in oncogenic transformation. Required for the growth of ERBB2 and RAS driven tumors.[UniProtKB/Swiss-Prot Function]