Dsg1b (NM_181682) Mouse Untagged Clone

CAT#: MC223399

Dsg1b (untagged) - Mouse desmoglein 1 beta (Dsg1b), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Dsg1b
• Synonyms: Dsg; dsg1-beta; Dsg5
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC223399 representing NM_181682
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACTGGCACTCCTTCAGGATAGCTGCCCTGCTGCTCACTTCCCTGGTGGTGCTTGAAGTTAACAGTG
  AATTTCAAATCCAGGTAAGAGATCATAACGCCAAAAATGGTACCATCAAGTGGCACTCCATCAGAAGGCA
  GAAAAGAGAATGGATCAAGTTTGCTGCAGCCTGTCGCGAAGGTGAAGACAACTCAAAGAGAAACCCAATT
  GCCAAAATTCATTCAGATTGTGCTGCAAACCAACCAGTAACATACCGCATCTCGGGAGTGGGAATTGACC
  AGCCACCTTATGGAATCTTCATTATTAACCAAAAAACTGGTGAAATCAATATAACATCCATTGTTGATCG
  TGAAGTCACCCCCTTTTTCATTATCTACTGCCGAGCACTGAATGCACAAGGTCAAGACTTGGAGAATCCA
  CTGGAGCTTAGAGTCAGAGTGATGGATATAAACGACAACCCTCCTGTGTTTTCAATGACTACATTTCTAG
  GACAAATAGAAGAAAATTCTAATGCAAACACACTGGTAATGAAACTCAATGCTACCGATGCCGATGAGCC
  AAATAACTTGAACTCAATGATAGCCTTCAAGATCATAAGACAGGAGCCTTCTGACTCACCAATGTTCATC
  ATCAACAGGAAAACTGGAGAAATCAGAACAATGAATAACTTTCTAGACAGAGAGCAATATAGCCAGTATT
  CCCTTGTGGTGAGAGGCTCAGACCGGGACGGTGGAGCAGACGGCATGTCTGCAGAGAGCGAGTGCAGCAT
  CACCATCCTGGATGTCAATGACAACATCCCATACTTGGAGCAGTCATCTTATGACATCGAAATTGAAGAA
  AATGCACTTCACTCACAGCTAGTGCAGATCAGAGTCATTGATTTGGATGAAGAATTCTCAGACAACTGGA
  AGGCAATCATTTTCTTTATCTCTGGAAATGAGGGAAATTGGTTTGAAATAGAAATGAATGAAAGAACAAA
  TGTAGGAACTCTGAAGGTTGTCAAGCCCCTAGATTATGAAGCCATGAAGAATCTACAGCTTAGTATTGGT
  GTTCGAAATGTAGCTGAATTCCACCAGTCAATTATTTCTCAATACAGACTCACAGCAACTATGGTCACTG
  TGACTGTGTTAAATGTCATCGAGGGCTCTGTGTTCCGTCCAGGTTCAAAGACATTTGTAGTAGACAGTAG
  AATGGAAGCAAATCACAGAGTGGGAGAGTTTGTAGCTACAGACCTGGACACAGGTCGAGCTTCCACAAAT
  GTTAGATATGAAATGGGAAATAACCCAGAGAACCTGCTGGTTGTTGACTCAAGAACAGGCATAATAACGT
  TAAGAAACAGAGTTACTATGGAACAATACCAAAGGCTTAATGGGGAATATAAAGGAACAGTGCTATCAAT
  AGATGATTCCCTTCAGAGGACCTGCACGGGGACAATCGTCATTGAACTTAGTGGCACTGGCTGGGTACCA
  GGTTCAGATGGTGGTGGCAGTTCCAGTGGCAGTGGTGGTAATCGTGACCCAGTGACTAATGGATATCAAG
  GCACTTCTACTGTGGGCCCGCAACGCGTCACAGGATCAGGGGGAGTCACTTCAAGTGGTGGAGGCAGTGG
  AGTTAACAACACTCCCGGTAGGCAAAATCCCTTAGACGAACCGGAACCCGAGCCCTTTGACATCACAGAA
  GACAACGTTCACTTCGGTCCTGCAGGCATCGGGCTGCTCATCATGGGGTTCCTAGTCTTAGGATTGGTTC
  CATTCCTGCTGATCTGCTGTGATTGTGGAGGGGCCCCTGGTGGTGGAGCTGGATTTGAACCTGTTCCAGA
  ATGTTCTGATGGAGCAATTCACACGTGGGCCATAGAAGGGCCACAGCCTGAACCCCACGATGGGATAACC
  ACCATCTGTGTGCCACAAATGCCACCTGGTAATGCCAATGTTATAGAATATATTGACAACTCAGGAGTTT
  ACACAAATGAATATTGTGGCAGAGAAATGCAAGATCTGGGAGGAGGAGAAAGAACCACAGGATTTGAACT
  AATGGATGGAGTTAAAACATCAGCTGCACCTGAGATATGCCAAGAATATTCAGGAACATTAAGAAGAAAT
  TCAATGAGAGAATGTAGAGATGGGGGTCTCAACATGAATTTCATGGAAAGTTACTTCTGCCAGAAAGCCT
  ATGCTTATGCGGATGAAGATGAAGGACGTCCATCCAATGACTGTTTGCTCATCTATGACATCGAAGGTGT
  GGGTTCCCCTGCAGGCTCCGTGGGCTGTTGCAGTTTCATTGGAGAAGATCTAGATGAGAGCTTCCTGGAT
  ACCTTGGGGCCCAAGTTTAAGAAGTTGGCAGATATCAGCCTGGGAAAAGAAATAGACTCATACCCAGATC
  CTGATTCTTCTTGGCCTCCTCAGAGCACTGAACCGATGTGTCCCCAGAGTACAGAGCCCCTGGGTAGTGG
  ATATCCACCTATATCCCCACATTTTGGTACGACCACAGTAATTTCTGAGAATGCCTACCCTTCTGGCCCT
  GGAGTACAGCATCCTTTGCCAATTCCTGATCCTCTGGGCTATGGCAATGTCACTGTAAGGGAGTCCTATG
  CCACCTCTGGCACTCTGAAGCCCTCTGTCCACTTTCATGATAACCAGCAGGCTTCAAATGTGGTGGTGAC
  AGAGAGGGTGGTGGGCCCCGTCCCTGGTGCTGATTTGCATGGCATGTTAGAGATACCTGACTTAAGAGAT
  GGAACAAATGTTATAGTGACAGAAAGGGTAATAGCACCAGGCTCAAGTCTACCCACTTCTCTGACCATTC
  CTAACCCTCGAGAGACCTCAAATGTAGTTGTGACAGAAAGAGTGATCCAACCAACCTCTGGCATGATAGG
  CAATCTGAGCATGACCCCTGAGTTATCAAGTGCCCAAAACGTGATTGTGACAGAGAGAGTGGTTTCTGGT
  GCTGGTATGAGTGGAATTGCTGGCACTGCCGGGTTAGGTGGGGTTGGAGGCATAGGCAGCAGTGGCCTTG
  TTAGCACCACCATGGGTGCTGCTGGCACTGGGCTGAATATGGGAGGAACAGCCACTATTGGCCACATGAG
  GAGTTCCTCTGACCATCACTTTAGTCAGACAATTGGATCTGCCTCCCCCAACATGGCTCGAAGTCGAATC
  ACAAAGTACAGTACGGTACAGTATAGCAAGTAG
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-RsrII
• ACCN: NM_181682
• Insert Size: 3183 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_181682.2, NP_859010.1
• RefSeq Size: 5952 bp
• RefSeq ORF: 3183 bp
• Locus ID: 225256
• UniProt ID: Q7TSF1
• Cytogenetics: 18 11.32 cM
• Gene Summary: This gene encodes a member of the cadherin family of proteins that forms an integral transmembrane component of desmosomes, the multiprotein complexes involved in cell adhesion, organization of the cytoskeleton, cell sorting and cell signaling. The encoded preproprotein undergoes proteolytic processing to generate a mature, functional protein. This gene is located in a cluster of desmosomal cadherin genes on chromosome 18. [provided by RefSeq, Jan 2016]