Zfp451 (NM_133817) Mouse Untagged Clone

CAT#: MC223398

Zfp451 (untagged) - Mouse zinc finger protein 451 (Zfp451), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Zfp451
• Synonyms: 4930515K21Rik; 4933435G09Rik; AI596398; COASTER; Kiaa0576-hp; mKIAA1702; Znf451
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC223398 representing NM_133817
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGAGACCCAGGGCCGGAGATAATAGAATCTGTCCCACCAGCTGGGCCCGAGGCATCAGAGTCTACCA
  CGGATGAAAACGAAGATGACATTCAGTTTGTTAGTGAAGGACCGTTAAGACCCGTTTTGGAATACATTGA
  TCTAGTCAGCAGTGATGATGAAGAGCCTAGTACCTCTCACAGTGATGAGAATTTTAAATGCAAAGACTAT
  ATAGATCACCAGAAGGATAAAGTTGCTTTAACTTTGGCTCGCCTGGCCCGCCATGTTGAAGTAGAAAAAC
  AACAGAAAGAAGAGAAGAACAGGGCATTTAGGGAAAAAATTGATTTTCAGCACGCTCATGGTTTACAAGA
  GTTGGAGTTTATTCAAGGACATTCAGAAACAGAAGCAGCAAGACAGTGTGTGGACCAGTGGCTAAAAATG
  CCAGGACTCAGAACAAATGCAGCTAATTCTGGAACAAAAAGATCATTCCAACGTGGAGGCAGGATGTGGA
  GGTCTGAGAAGCCAATTTTGTGTCCTATAATGCACTGTAACAAGGAATTTGACAATGGGCACCTTCTGTT
  GGGACATTTGAAAAGGTTTGATCACTCCCCATGTGATCCCACAATTACGTTACATGGACCTCTGGCTAAT
  TCGTTTGCATGTGCAGTGTGCTATGAACATTTCGTTACTCAACAGCAGTACAAGGACCATCTTCTCTCCA
  GGACAGCTGCAGCCGATGGACATAGCAATAGCCTTCTTCCTCAGATTATTCAATGTTATGCTTGTCCACA
  GTGCTTCCTTTTGTTTAGCACCAAGGATGAGTGTTTGAAGCATATGTCTACAAAGAATCATTTCCATCAG
  AGCTTTAAACTGAGTGATAATAAAGGAACGGCCCGCCCAATATCATTTCCATCTTTTGCAAAGAAGCGTT
  TGGTCTCTCTGTGCAAAGATGTTCCATTTCAGGTTAAGTGTGTGGCCTGCCACCAGACTCTGCGTTCTCA
  CATGGAGCTCACTGCCCATTTCAGGGTTCGTTGTCAAAATGCTGGACCTGTTGCTATAGCTGAAAAAAGC
  ATTACTCAGGTTGCAAAAGAATTCATAGTAAGAGGTTATTGTTCAGATTGCAACCAGGTCTTTATGGATG
  TAGCCAGCACCCAAAGTCACAAGAATTCAGGACACAAAATTACACTTGCGAACTCGGTGGAAGAATCTGT
  CTTGCTTTATTGCCACATCAGTGAAGGGAGTCGGCCTCCTTGTGATTTACATTTATTTAGTCAACCAAAA
  ATTTCATCACTTAAACGGATTCTGTCCGTTAAAGAGTCCAGTGCAGAGGATTGTATCGTTCCGACAAAGA
  AGGTGAACTTAGGTGTTGAAAGCCTTGGAGGTGCAACTCGTGTGCAGAGGCAGAGTCCAGCAGTCACAGC
  CTGGTTTTGTGAATGCAGACGGCAGTTTCCCAGTGAAGAGGCGGTAGAAAAGCATGTTTTCTCGGCAAAC
  ACAATGTGTTATAAGTGTGTGGTCTGTGGAAAGGTTTGTGAAGATTCGGGGGTCATGCGTTTACACATGA
  GCCGGTTTCATGGAGGGGCGCATTTAAATAACTTTCTATTTTGGTGTCGGACGTGCAAAAAGGAGTTAGT
  AAAGAAAGATGCCATCATGGCACACATTACTGAGTTTCATAGTGGGCATAGATATTTTTATGAGATGGAT
  GAGGTAGAGGAGGAAGAGGAGGAGGCCATGCCGTCATCCTCTGTGGAGAGCCATTTGAATACTGACAAAC
  CTCCTTCACCCATTGCTGTTGTTGATCACTGCCCGGCCAACAGTCCTCCCAGGGGCAGGTGGCAGTGCCG
  CATCTGTGAGGACATGTTTGAATCCCAGGAGTGTGTGAAGCAGCACTGCATGTCCTTGACAAGCCACCGG
  TTTCACAGATACAGCTGTGCCCACTGCAGAAAGACTTTCCATAAAGTGGAAACTCTGTACCGACATTGCC
  AGGACGAGCATGACAGTGAGATCATGATGAAGTACTTCTGTGGGCTTTGTGATCTCATTTTTAACAAGGA
  AGAAGAATTTCTGAGTCACTATAAGGAACACCACAGCATAGATTATGTGTTTGTGTCAGAAAAAACTAAA
  ACCTCAATTAAAACTGAAGGCGATTTTAAAATAGTAGAGACCAGTAGTTTACTAAGCTGTGGCTGTCATG
  AGAGTTACATGTGTAAAATCAACAGAAAGGAAGACTATGACCGATGCCTTCCAGTCCTGCTGGAGAAAGG
  TAGGCTGTGGTTTCGCTGCAGCTCGTGCTCAGCCACAGCACAGAATGTGACCGACATTAACACTCACGTC
  TGCCAAGTGCACAGAAAAGAGAAAAGTGAGGAGGAGCAGCAGTATGTGATCAAGTGTGGCATCTGCACCA
  AGGCATTCCAGAACACGGAGAGCGCTCAGCAGCACTTCCACAGGAAGCACGCGGCCCTCCAGAAACCCAC
  CGCGACCCCAGGGGGAGCCAACAGAAGCAGCACATGCCAGCTGGCTGCTAGTGCCTCACATGCTGAGAAA
  AACCTGAAACAGCCTAGCTCTCAGAAACATTCAGACGTGGAAAAAGGAGCTGAGCATGATGTACGCTGCC
  AGAACATAGAGGAGGAAGTTGAGCTTCCGGATGTGGACTACCTGCGGACCATGACTCACATAGTCTTTGT
  AGATTTTGATAACTGGTCCAACTTTTTTGGTCATCTACCTGGGCATCTTAATCAAGGAACGTTTATTTGG
  GGCTTTCAAGGGGGAAACACCAACTGGAAGCCCCCGCTCAGCTGTAAGGTCTATAATTACTTGAGCAGGA
  TTGGCTGCTTCTTCCTTCATCCTCGCTGCAGTAAAAGAAAAGATGCCGCGGATTTTGCCATATGTATGCA
  TGCTGGCCGTCTAGATGAGCAACTTCCCAAACAAATTCCTTTCACCATCCTCTCGGGAGACCAAGGCTTT
  CTGGAGCTAGAGAATCAATTTAAGAAGACCCAGAGGCCAGCTCATATACTAAACCCTCACCACTTAGAGG
  GAGACATGATGTGTGCCTTGTTAAATAGCATATCTGATACTACCAAAGAGTGTGACAGTGACGATAGCTC
  GGGGATGAAAGGATCTCCAGCAGAGGAGCTCAGGGCCACGGAGGATGTGGAATTAGAAGAAGCTATTAGA
  AGAAGTCTTGAGGAGATGTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_133817
• Insert Size: 3171 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_133817.3, NP_598578.1
• RefSeq Size: 4030 bp
• RefSeq ORF: 3171 bp
• Locus ID: 98403
• UniProt ID: Q8C0P7
• Cytogenetics: 1 12.81 cM
• Gene Summary: E3 SUMO-protein ligase; has a preference for SUMO2 and SUMO3 and facilitates UBE2I/UBC9-mediated sumoylation of target proteins. Plays a role in protein SUMO2 modification in response to stress caused by DNA damage and by proteasome inhibitors (in vitro). Required for MCM4 sumoylation. Has no activity with SUMO1 (PubMed:26524493). Preferentially transfers an additional SUMO2 chain onto the SUMO2 consensus site 'Lys-11'. Negatively regulates transcriptional activation mediated by the SMAD4 complex in response to TGF-beta signaling. Inhibits EP300-mediated acetylation of histone H3 at 'Lys-9'. Plays a role in regulating the transcription of AR targets (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1).