Usp28 (NM_175482) Mouse Untagged Clone

CAT#: MC223359

Usp28 (untagged) - Mouse ubiquitin specific peptidase 28 (Usp28), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Usp28
• Synonyms: 9830148O20Rik; AU022237; mKIAA1515
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC223359 representing NM_175482
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACTGCGGAGCTGCAGCAGGACGACTCGGCGGGGGCGGCGGACGGCCATGGCTCGAGCTGCCAGATGC
  TGTTAAATCAGCTGCGAGAAATCACAGGCATCCAGGATCCTTCCTTTCTTCATGAAGCATTAAAGGCCAG
  TAATGGTGACATCACCCAGGCTGTCAGCCTTCTCACAGACCAAAGAGTTAAGGAGCCCAGTCACGACACA
  ACTGCTGCAGAACCATCTGAAGTAGAAGAGAGTGCTACCAGCAAAGATCTCTTAGCAAAAGTGATAGACC
  TCACTCACGACAACAAAGATGACCTGCAGGCAGCCATTGCCTTGAGTCTCCTGGAGTCTCCTAACATTCA
  GGCTGACAACAGAGATCTTAATAGAGCGCATGAGGCTAACTCTGCAGAAACTAAACGCTCAAAGAGAAAA
  CGCTGTGAAGTTTGGGGAGAAAACCACAATCCCAATAACTGGAGGAGAGTGGATGGTTGGCCAGTTGGGC
  TGAAAAATGTTGGCAACACATGTTGGTTCAGTGCTGTTATTCAGTCTCTCTTTCAGTTGCCTGAATTTCG
  AAGACTTGTTCTCAGTTATAACCTGCCACAGAACATCCTTGAAAATTGTCGAAGTCACACTGAAAAGAGA
  AATATCATGTTTATGCAAGAGCTTCAGTATTTATTTGCTCTGTTGTTGGGATCAAATCGAAAATTTGTAG
  ACCCTTCTGCAGCCCTGGATCTACTGAAGGGAGCCTTCCGATCATCCGAGGAGCAGCAGCAAGATGTGAG
  TGAATTCACACACAAGCTCCTGGATTGGCTGGAGGATGCGTTCCAGCTAGCTGTTAATGTTAACAGCCAT
  CTCAGGAATAAATCTGAAAATCCCATGGTGCAGCTATTCTACGGTACTTTTCTGACAGAAGGGGTCCGAG
  AAGGAAAGCCCTTTTGTAACAATGAGACCTTCGGCCAGTATCCTCTTCAGGTAAACGGTTACCACAACTT
  AGACGAATGTTTGGAAGGGGCCATGGTGGAAGGTGACATCGCGCTGCTTCCTTCGGATCGTTCCGTGAAG
  TATGGGCAGGAGCGTTGGTTTACAAAGCTGCCTCCAGTGTTGACCTTTGAACTCTCCAGATTTGAGTTTA
  ATCAGTCTCTTGGGCAGCCTGAAAAAATTCATAATAAGCTGGAATTTCCTCAGATTATCTATATGGATAG
  GTACATGTATAAGAGCAAAGAGCTTATCCGAAGTAAGAGAGAGAGCGTTCGGAAGTTGAAGGAGGAAATA
  CAAGTTCTGCAGCAGAAGTTAGAAAGGTATGTGAAGTACGGCTCGGGCCCATCTCGGTTCCCACTTCCCG
  ACATGTTGAAATACGTTATTGAATTTGCCAGTACAAAACCTGCCTCTGAAAGCTGTCTGTCTGGAAGTGC
  TGAGCATGTGACATTACCACTTCCCTCGGTGCACTGCCCGATTTCTGACCTCACACCCAAGGAAAGTTCA
  AGTCCAGAAAGCTGCTCTCAGAATGCTGGAAGTACCTTTTCCTCTCCAGAAGATGCTCTTCCCAGCTCTG
  AGGGGATGAATGGGCCATTTACCTCTCCTCACTCGTCTCTGGAAACGCCTGCACCCCCAGCTCCTCGGAC
  AGTCACAGATGAAGAAATGAACTTCGTTAAGACCTGTCTTCAGAGGTGGCGGAGTGAAATTGAACAGGAT
  ATACAAGACCTAAAGAACTGTATCTCGAGCAGCACGAAGGCGATTGAGCAGATGTACTGTGACCCTCTTC
  TTCGTCAGGTGCCTTATCGCTTACATGCAGTTCTTGTTCACGAAGGACAAGCCAGTGCAGGACACTACTG
  GGCATACATCTACAACCAGCCCCGGCAGACCTGGCTCAAATACAACGACATCTCTGTTACTGAGTCTTCC
  TGGGAGGAACTTGAAAGAGATTCTTACGGGGGCCTGAGAAATGTCAGCGCCTACTGCTTGATGTATATCA
  ATGACAACTTGCCCCACTTCAGTGCAGAGGCATCTTCTAATGAATCTGATGAGACTGCAGGAGAAGTGGA
  AGCCCTGTCTGTGGAACTCAGGCAGTACATTCAGGAAGATAACTGGAGGTTTCAGCAAGAGGTGGAGGAA
  TGGGAGGAAGAACAGTCCTGTAAGATCCCACAGATGGAGTCCTCCCCGAACTCTTCCTCACAGGATTTCT
  CCACATCACAAGAGTCTCCAGCAGTGTCCTCTCATGAGGTCCGATGCTTGTCTTCTGAGCATGCTGTGAT
  TGCAAAAGAACAGACTGCCCAGGCAATTGCCAACACAGCTCATGCCTACGAGAAGAGTGGTGTAGAAGCA
  GCCTTGAGTGAGGCATTTCATGAGGAGTACTCCAGGCTCTATCAGCTTGCCAAAGAGACACCCACCTCTC
  ACAGTGACCCTCGCCTTCAGCATGTTCTTGTCTACTTTTTCCAAAATGAAGCACCCAAGAGGGTAGTAGA
  ACGGACCCTTCTGGAACAGTTTGCGGATAGAAACCTTAGCTATGATGAGAGATCTATCAGTATCATGAAG
  GTGGCTCAGGCCAAATTGATGGAAATTGGTCCAGATGACATGAACATGGAAGAGTATAAGAGGTGGCATG
  AAGATTATAGTCTGTTCCGAAAGGTGTCTGTGTACCTTCTAACGGGCCTGGAACTCTTCCAAAAAGGAAA
  GTACCAGGAAGCATTGTCCTACCTGGTGTACGCATACCAGAGCAACGCTGGCCTGCTGGTGAAGGGGCCC
  CGAAGGGGCGTGAAGGAGTCTGTGATTGCCTTATACCGCAGAAAATGCCTTCTGGAGCTGAATGCCAAGG
  CTGCTTCTCTTTTTGAAACAAATGACGACCATTCTGTAACAGAGGGCATTAATGTAATGAATGAGTTGAT
  CATTCCCTGCATCCACCTGATCATTAATAACGACATCTCCAAGGACGACCTCGATGCCATCGAGGTCATG
  AGGAACCACTGGTGCTCTTACCTGGGGAAGGATATTGCAGAGAATCTGCAGCTGTGCTTAGGAGAGTTCC
  TACCCAGGCTTCTAGATCCTTCTGCAGAAATCATCGTCCTAAAGGAGCCTCCAACTATTCGACCGAATTC
  TCCCTATGATCTTTGCAACCGGTTTGCAGCTGTCATGGAGTCCATTCAAGGAGTTTCAACAGTAACCGTG
  AAATAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_175482
• Insert Size: 3156 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_175482.3, NP_780691.2
• RefSeq Size: 4220 bp
• RefSeq ORF: 3156 bp
• Locus ID: 235323
• UniProt ID: Q5I043
• Cytogenetics: 9 A5.3
• Gene Summary: Deubiquitinase involved in DNA damage response checkpoint and MYC proto-oncogene stability. Involved in DNA damage induced apoptosis by specifically deubiquitinating proteins of the DNA damage pathway such as CLSPN. Also involved in G2 DNA damage checkpoint, by deubiquitinating CLSPN, and preventing its degradation by the anaphase promoting complex/cyclosome (APC/C). In contrast, it does not deubiquitinate PLK1. Specifically deubiquitinates MYC in the nucleoplasm, leading to prevent MYC degradation by the proteasome: acts by specifically interacting with FBXW7 (FBW7alpha) in the nucleoplasm and counteracting ubiquitination of MYC by the SCF(FBXW7) complex. Deubiquitinates ZNF304, hence preventing ZNF304 degradation by the proteasome and leading to the activated KRAS-mediated promoter hypermethylation and transcriptional silencing of tumor suppressor genes (TSGs) in a subset of colorectal cancers (CRC) cells.[UniProtKB/Swiss-Prot Function]