Uba6 (NM_172712) Mouse Untagged Clone

CAT#: MC223347

Uba6 (untagged) - Mouse ubiquitin-like modifier activating enzyme 6 (Uba6), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Uba6
• Synonyms: 4930542H01; 5730469D23Rik; AU021846; AW124799; E1-L2; Ube1l2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC223347 representing NM_172712
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGCGGTCCGAGCCGTTGGCTGTGCTTTCCTGTGAAGAGGCGTCCTGCTCCTCCTGGGGAGCCTGCG
  GTGCAAGTAAAAATTTACCTACCATGACAACAGAATCTTTGGAAATCGATGATGGATTATACAGTCGCCA
  GAGATATGTTCTTGGTGACACAGCAATGCAGAAGATGGCCAAGTCCTGTGTCTTCTTAAGTGGTATGGGT
  GGTCTTGGAGTGGAAATTGCAAAGAATCTTGTTCTTGCAGGGATTAAGGCCCTAACAATTCATGATACAA
  AAAAATGCCAAGCATGGGATCTAGGGACCAATTTCTTCCTGTGTGAAGATGATGTTGTTAATGAGAGAAA
  CAGGGCTGAAGCTGTACTTCATCGTATTGCAGAACTAAATCCATATGTTCAGGTCTCATCATCCTCTGCC
  CCTCTTGATGAAACCACAGACCTCTCTTTCTTAGAAAAATATCAGTGTGTAGTATTGACTGAAATAAAAC
  TGACATTACAAAAGAAGATCAACAATTTTTGCCATTCTCATTGCCCTCCAATTAAGTTCATTAGTGCAGA
  TGTACATGGAATTTGGTCCAGGTTGTTTTGTGATTTTGGTGATGAATTTGAAGTTTCAGATACAACAGGA
  GAAGAACCAAAAGAAATTTTCATTTCAAACATAACGCAAGCTAATCCAGGTATTGTCACTTGCCTTGAAA
  GTCATCCTCACAAGCTTGAGACAGGACAGTTCCTAACATTTCGAGAAATTCATGGAATGACAGGCTTAAA
  TGGATCTGTACAACAGATAACTGTCATATCACCATTTTCCTTCAGCATTGGTGATACTACAAAACTGGAC
  CCATATTTACATGGAGGCATAGCCGTGCAGGTGAAGACTCCTAAAACATTCTGCTTTGAACCCCTGGAGA
  GCCAGATAAAACATCCAAGGTGCCTTATTGCAGATTTTAGCAAACCTGAGGCACCTTTAGAGATTCATCT
  CGCTATGCTTGCCTTGGACCAGTTTCAGGAGAACTATAACCGTAAGCCAAATATCAGATGTCAGCAGGAT
  TCAGATGAACTATTGAAGCTAACAGTATCTATAAATGAAACCTTGGAAGAGAAGCCTGAAGTGAATGCTG
  ACATTGTGCACTGGCTTTCGTGGACTGCCCAAGGCTTTTTACCACCACTCGCTGCTGCAGTTGGAGGTGT
  TGCTAGCCAAGAAGTCTTAAAAGCTGTGACGGGGAAGTTCTCGCCCTTGTGTCAATGGTTGTACCTTGAA
  GCAGCAGATACTGTGGAATCCCTAGGCAATCCTGGGCATGAAGAGTTTCTCCCACGAGGAGATAGATATG
  ATGCCATACGTGCCTGCATTGGAAATACATTGTGTCAGAAGCTACAAAATTTGAATATCTTCTTAGTAGG
  ATGTGGTGCCATAGGCTGTGAAATGTTAAAAAATTTTGCATTACTGGGTGTTGGTACAGGCAGAGAGAAA
  GGAATGGTAACAGTTACAGATCCTGACTTGATAGAAAAATCCAATTTAAACAGACAGTTCCTGTTTCGTC
  CTCATCACATACAGAAACCTAAAAGCTATACTGCTGCTGAAGCAACTCTGAAAATAAATCCTCAATTAAA
  AATAGATGCACACCTGAACAAAGTTTGTCCAGCCACTGAGAGTATATACAGTGATGAATTTTACACTAAG
  CAAGATATCATTATTACAGCACTAGATAATGTGGAAGCCAGGAGATATGTAGACAGTCGCTGTTTAGCAA
  ATCTACGGCCTCTCTTAGATTCTGGAACAATGGGCACCAAGGGACATACTGAAATTATCGTACCTCAATT
  AACTGAATCTTATAATAGTCATCGAGACCCCCCCGAAGAGGAAATACCATTTTGCACTCTAAAGTCCTTT
  CCAGCAGCTATCGAACATACTATCCAGTGGGCAAGAGACAAGTTTGAAAGTTCCTTCTCCCACAAACCTT
  CACTGTTTAACAAGTTTTGGCAAGCATATCCCTCTGCAGAAGATGTCTTACAGAAGATACAAAATGGACA
  AAGTTTAGAAGGCTGTTTTCAAGTTATTAAGCTACTTAGCAGAAGACCTAGAATTTGGTCCCAGTGTGTA
  GAGTTAGCAAGACTAAAGTTTGAAAAATACTTTAACCATAAGGCTCTGCAGCTTCTTCACTGTTTTCCTC
  TTGAGACAAGACTAAAAGATGGCAGTTTGTTTTGGCAATCACCAAAAAGGCCGCCTTCTCCAATAAAATT
  TGATTTAAATGAACCTTTGCACCTCAGTTTTCTTCAGAGTGCTGCAAAACTATATGCTACAGTATATTGT
  ATTCCGTTTTCAGAAAAGGACTTATCAGTGAATAGCCTCATGGACATTCTTTCAGAAGTTAAAATTGAGG
  AATTCAAGCCTTCCAACAAGGTTGTTCAAACAGACGAAACTGCAAGGAAGCCCGACCATGTTCCTGTCTC
  CAGTGAAGATGAAAGGAATGCTGTTTTCCAACTAGAAGAGGCTCTTTCCTCTAACAAAGCTACCAAAAGT
  GACCTTCAGATGACAGTGCTTTCATTTGAAAAAGATGATGACCGTAATGGACACATAGATTTCATCACAG
  CTGCTTCCAACCTTCGAGCCAAAATGTATAGCATTGAACCAGCTGACCGTTTTAAAACTAAACGCATAGC
  TGGTAAAATTATACCTGCTATAGCAACATCTACTGCTGCAGTTTCTGGCTTGGTTGCTTTGGAGATGATC
  AAGGTAGCTGGTGGCTATCCCTTCGACGCTTACAAAAACTGTTTCCTTAATTTAGCCATTCCAATAATAG
  TGTTTACAGAGACATCTGAAGTAAGAAAAACTGAAATCAGAAATGGAATATCATTTACAATTTGGGACAG
  ATGGACTGTACATGGAAAAGAAGATTTCACCCTCTCAGATTTCATAAATGCTGTCAAAGAAAACTATGGA
  ATTGAGCCGACAATGGTGGTGCAGGGAGTCAAAATGCTTTATGTCCCTGTAATGCCAGGTCATGCAAAAA
  GGCTAAAGTTAACAATGCACAAACTTGTGAAGCCTTCCACTGAGAAGAAATATGTGGATCTTACTGTGTC
  ATTTGCCCCAGACGCTGATGGAGATGAAGATTTGCCCGGCCCTCCAGTGAGATACTACTTCAGTCATGAC
  ACTAATGAGTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_172712
• Insert Size: 3162 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_172712.2, NP_766300.1
• RefSeq Size: 5101 bp
• RefSeq ORF: 3162 bp
• Locus ID: 231380
• UniProt ID: Q8C7R4
• Cytogenetics: 5 E1
• Gene Summary: Activates ubiquitin by first adenylating its C-terminal glycine residue with ATP, and thereafter linking this residue to the side chain of a cysteine residue in E1, yielding a ubiquitin-E1 thioester and free AMP. Specific for ubiquitin, does not activate ubiquitin-like peptides. Differs from UBE1 in its specificity for substrate E2 charging. Does not charge cell cycle E2s, such as CDC34 (By similarity). Essential for embryonic development. Required for UBD/FAT10 conjugation.[UniProtKB/Swiss-Prot Function]