Cd101 (NM_001167906) Mouse Untagged Clone

CAT#: MC223302

Cd101 (untagged) - Mouse CD101 antigen (Cd101), transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Cd101
• Synonyms: EWI-101; Gm734; Gm1016; Igsf2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC223302 representing NM_001167906
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCATGCATCCTGTGTGTGGCATCTCTCTTTCTCTCTCTAACTAAGTTCAGCATCGGCCAGAGGGAAG
  TAAAAATTCAAGAAGGCCCTCTGTACAGAGCCGAAGGTTACCCTGTCAGCATCAGGTGCACCGTAAGTGG
  TCATCAGGGTCCTTCCACGCAGGATTTCCGGTGGTCTATTTACCTGCCAAGCGCCCCGACCAAGGAAGTC
  CAGATCATCAGTACCAAGGATGCCGGCTTCTCTTACGCAGTGTATGCCCAGAGGGTGCAAAGCAAGGAGA
  TCTACATAGAGAGGCTGCAGGGTGACTCGGTCCTGCTGCATATCTCAAAACTCCAGATGAAAGATGCTGG
  CGAGTACGAGTGCCACACACCCAACACGGATGGGAAGTACTTTGGAAGTTACAGTGCAAAGACAAATCTT
  ACTGTGGTTCCCGACACCCTGTCTGCCACCATGCCCTCCCAGACGCTCAGTAAGAAGGAAGGTGAGCCCT
  TGGAACTCACCTGTGAGACAACCAAAGCCACCGTGCAACACACCCATCTCTCTCTCACCTGGTACCTGAT
  GCAGGAAGGAGGAGGGAGCCAAGCCACTGAGATCGTTTCTCTCTCCAAGGACTTTGTATTGACCCCTGGG
  TCCTCCTATGCAGACAGGTTTGTGGCCGGTGATGTACGGCTGGACAAGCTTGGAGCCACTTCCTTCAGGC
  TGTCTGTAGGCAAGCTCCAGCCCTCAGATCAGGGCCAGGTGTTCTGTGAGGCCACAGAATGGATTCAGGA
  TCCAGATGAAACGTGGACTTTGATCACAAGAAAGCAGACAGATCAAACAGCTCTGAGGATCCAGCCGGCA
  GCAAGAGATTTTACAGTGAGCATCACAGCCAGTAGCTCACCTGATGAAGGAAAACCCTTGGAACTGGTTT
  GCCTGGCTGTTGGCAGAGATGGTAACCCGCAGCTTCAGGGTGTGTGGTTTCTCAATGGGAAGGAAATTGC
  CCAGACTGATGCTGGTGGGGTCCTGGACCTGAAGAGAGACTACAGAGACAGAGCGAGCCAAGGCCAGCTG
  CAGGTGTCAAAGTTAAGTGCCCAGACGTTCTCTCTCAAGATCTTCTCCGTGGGTCCAGAGGATGTAGGCA
  CCTACAGTTGTGAAGTGGCAGAGGTGGCGAGGACTCAGATGGGCTCCTGGCAGGTACTTCAGAGAAAGCA
  GTCACCAGGCTACCGGGTGCAGCTGAGGGAGCCAGCAGCAAGAAGTGTGACCGTGTCTGCCGAGCAGCGT
  ACTGTGTGGGAAGGAGAGACGCTTACCCTTCTCTGCAAGGCAGCTGGGGATGTGAGTGCTCTATCTGTGA
  GTTGGTGGCTCACCCCACAGGACCAGTCCACACCCGTGTTTGTGGCTGGCATGGGGCAAGATGGTACTGT
  GCAGCTGGGAGTCTCTTCTCCGGGCCCTGCGCACCGTGGTAACAGGAGGCTGGAGAAAGTGGACTGGGCC
  ACCTTTCGGCTGGAGATTGCCTCTGCCATGGTCACAGACAGCGGTACCTATGAATGCAGGGTGTCAGAGA
  GACTCCAGAACCAGGCCAAAGGTTTGCAGTCAACCCAGAAGATTTCAGTCACTGTAAAATCTCTGAAGTC
  AAGTTTACGAGTTAATCTGATGAGCCGTCAGCCACAAGTGATGCTAGCCCATACCTTCCACCTGTCCTGT
  GTAGTGAGGGCCAACTACTCGGATCTCAAGCTGCCGTTCTCGGTAACGTGGCAGTTCCAGCCAGCGGGCT
  CTGGAGCCTTTCATCGGCTTATTCGAATTGCCCACAATGGCACCGTTGAATGGGGGGACGTCCTCTCCCA
  GATCCACAGGAAGACGAAGGTGTCACAGTCTTTCTTTCGTTCTCAACTCCAAATCTACGATGCAGCTATG
  GAGGAGACAGGGGTGTATCGGTGTACAGTGGAGGTTTATGACAGAGATTCCATATGCACAAGTGGCCCAG
  CCAGAGTATCTGCCACCTCTAACCTATTAATGATTACTGTCACCTTTCCAGAGAGCAAACTGAGCGTGAA
  CTCAAGCAGTCAGGTCCAAGAGCTATCCATCAGCTCCAGCACTCAGATAGAATGTGCCATCTTGTCCCGA
  TCTGCTGGAAACCTTCCGTTATCCATCATTTGGTACTTCTCTTCCGTTTCTGCAAATGCATCCTATCTGA
  AAATCCTAGAAATGGACCAAAGCAGTGTTGTAAAATATGGGGACGAATTTCAAACCCCTCGGAGCAAGCA
  AAAATTTTATTCTGAGAAAGTTTCTCAAGACTTATTTCTGCTGAACATTCTGAGTGTGGAGGACAGTGAC
  CAGGGCCACTACCACTGTGCTGTAGAGGAATGGCTCTTATCTACAAATGACACTTGGCAAAAGCTTGAAA
  GAAAGACTTCAGGACTCACAGAATTGAAACTCAGGCCCACAGGAAGCCAGGTCCATGTCTCCAAAGTGAA
  TTGGACAGGAAACGCTACCGAGTATGGAGAGGCGGGCTTCAGCTGCAGCCTAGATGGCTCAGGCAGCACA
  GCTTCCCTATACTCTGTGACATGGTACCGGGGCAGAGGAACTGCCACGGCTACTGCTGCCGCAGTTGCCA
  ATGCCACTGCCACCATCACTGCCCCCGCAGGGAGTCAAATGCTGGTGCACCTGCAGTATGACGGCTTGCT
  GCAGTATGGCAGAGAGGGGAGCAGGAGGCTCCAGCACTGCTACCGATCCTCTCCCACAGACTTCGTCCTG
  AAGCTGCATCGGGTGGAAATGGAGGATGCTGGGATATATTGGTGCAGGGTGACTGAGTGGCAGCAACATG
  GCCACCCAGGCAAGTGGATCAACCAAGCCTCAGGCGAGTCGCAACGAATGGTGCTCAGGGTGCTGCGCTC
  AGAGCCCACGGTCTCCTCCCTGATCTGCTCCTCGGGGCCCTTGCTCCACTTCCTCATCGTCTGCCCCTTC
  GTCATGCTGCTCCTTCTGGCCACGTCCTTCCTCTGCTTGTACCGGAAGGCCAGGAAGTTGTCACAGCTGA
  GTCTCAGTGCAAAGAAAGAAAAGGCTCTCTGGGTGGGCATGAGAAAGACATCACTTCAGAAGGAAGCTGG
  AGAGGAGAGTGGACACTACTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001167906
• Insert Size: 3102 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001167906.1, NP_001161378.1
• RefSeq Size: 3254 bp
• RefSeq ORF: 3102 bp
• Locus ID: 630146
• UniProt ID: A8E0Y8
• Cytogenetics: 3 F2.2
• Gene Summary: Plays a role as inhibitor of T-cells proliferation induced by CD3. Inhibits expression of IL2RA on activated T-cells and secretion of IL2. Inhibits tyrosine kinases that are required for IL2 production and cellular proliferation. Inhibits phospholipase C-gamma-1/PLCG1 phosphorylation and subsequent CD3-induced changes in intracellular free calcium. Prevents nuclear translocation of nuclear factor of activated T-cell to the nucleus. Plays a role in the inhibition of T-cell proliferation via IL10 secretion by cutaneous dendritic cells (By similarity).[UniProtKB/Swiss-Prot Function]