Thsd4 (NM_001040426) Mouse Untagged Clone

CAT#: MC223215

Thsd4 (untagged) - Mouse thrombospondin, type I, domain containing 4 (Thsd4), transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Thsd4
• Synonyms: ADAMTSL-6; Adamtsl4; ADAMTSL6; AI585901; B230114P05Rik
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC223215 representing NM_001040426
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTTTCCTATCTCACGAGCTGTCTCAGTGCCCTGAGCACCCTCCTGCTGCTGCTTGGTTCTCAGCTTG
  TCTGCCCGCAGCCTTCCACTGAACACAGAAAGGTCCCACAACGGATGGCAGTGACCGAGGGAACCCCAGA
  GGACAGTGGCAGTGGCTCCCCAGGTGTGTGGGGCTCCTGGGGCCCCTGGTCTGCCTGTTCTCGCAGCTGC
  AGTGGTGGTGTGATGGAGCAGACACGGCCCTGCCTGCCCAGCTCCTACCGTGCTCGAGGAGGCTCCCGGC
  CCAATGGCCGTGCACTGTCCATCACTGGCCACGTTGTGTCAGCCGTACGGACCTCAGTACCACTGCATCG
  AAGCCAGGAGGACCAAAGAGCCCTGGCTGGCTCCAATGCTAGCCGCCAAGGCCCTGCAGTGGTGCGAGGC
  AGCCGACATCCACAGGCCCGGGGCCGTGAACCCTCAGAGAGAAGGAGCAGGACCCGAGGTCCTATTGGCC
  CTGGCAAGTATGGCTATGGGAAGGCTCCCTACATCTTACCACTGCAGACGGATACCACGCATACTCCACA
  GAGGCTTCGGAGACAGAGGCCTTCATCCAGGCACTCCAGGTCCCAGGAGGCATCTGCTTCTAAGCAGGGC
  TATCGGCCACCAACCCATCAGTTCTCCCACAGCCAACCTCTCTACCAGAGTGACAGTGGCCCTCGCTCTG
  GACTGCCACCTTCTGAGGCTTCTATCTACCAGCTGCCCTTGACTCACGACCAAAGCTATCCTGCAGCGTC
  GAGTCTCTTCCACAGACCGGAACTGAGCAGCCACCATGGTGCTAGGCCCCACGGGGCAGCTCAAGCCTTT
  CCCCAGCATCTGCGATCAACAGCAATCTCCTGCATCGGGGCCTATCGGCAGTACAAACTGTGCAACACCA
  ATGCGTGTCCAGAAAGTGGTAGAAGTATCCGGGAGGTACAGTGTGCATCCTACAACAACAAGCCATTCAT
  GGGCCGGTTTTATGAATGGGAACCATTTGCAGAAGTAAAGGGTAATCGCAAGTGTGAGCTGAACTGCCAG
  GCAACCGGCTACCGCTTCTACGTCCGGCAAGCTGAGAAGGTCATCGATGGCACACCCTGTGATCAGAATG
  GCACGGCAATCTGTGTGTCGGGGCAGTGCAAGAGCATTGGCTGTGACGACTTCCTGGGATCTGACAAAGT
  CTTGGACAAGTGCGGGGTGTGTGGAGGTGATAACACGGGCTGCCAGGTGGTGTCGGGAGTGTTCAAGCAC
  GCCCTCACCAGCCTGGGCTACCACCGGGTTGTAGAGATTCCCCAAGGAGCTACGAAAATTAACATTACAG
  AGATGCACAAGAGCAACAACTACTTAGCCTTGCGAAGTCGCTCTGGCCGCTCCATCATCAATGGGAACTG
  GGCAATCGATCGCCCGGGGAAGTACGAGGGCGGAGGGACAATGTTCACCTACAAACGACCGAACGAGGTC
  TCCAGCACTGCGGGGGAGTCCTTTCTGGCTGAGGGTCCCACTAATGAGATCCTGGATGTCTATATGATAC
  ACCAGCAGCCCAACCCAGGAGTCCACTATGAGTACGTGATCATGAGGAATAATGCCATCAGCCCACAGGT
  GCCACCACACAGGAGACCAGGAGAACCTTTCAATGGGCAGCTGGAAGAGGAAGACAGAGGCCAGGAGGAC
  AGAGAAGAGAGGGAGAAGAACCAGGAAAAGGAAGACTCACAAGTCGAGGCCCCTGAGGTATTCACGTCAG
  AATCCACGCAGACCTTCCCAGTCAGGCATCCGGAGAGATTCCCTTCCCACCGACCAGACAACTTGGTGCC
  CCCAGCACCACAGCCCCCAAGGCGAAGCCGGGACCACAACTGGAAGCAGCTGGGGACAACAGAATGCTCC
  ACCACCTGTGGGAAAGGATCGCAGTACCCTATCTTCCGCTGCGTGCACAGAAACACACACGAGGAGGTTC
  CCGAGAGCTACTGTGACTCCAGCATGAAGCCGACCCCAGAGGAGGAACCCTGCAACCTCTTCCCCTGCCC
  AGCCTTCTGGGACATCGGGGAGTGGTCGGAGTGCAGCAAGACCTGTGGTCTGGGCATGCAGCACCGCCAG
  GTCCTGTGTCGCCAGGTGTATGCCAACCGTAGCCTGACGGTGCAACCCTACCGCTGCCAGCACCTGGAGA
  AGCCAGAGACCACCAGCACCTGCCAGCTCAAGATCTGCAGCGAGTGGCAGATTCGGACCGACTGGACCTC
  GTGCTCAGTGCCCTGTGGCGTGGGGCAGCGGACCCGAGACGTGAAGTGTGTGAGCAACATCGGGGACATG
  GTGCATGACGAGGAGTGCAACATGAAGCTCCGGCCAAATGACATCGAGAACTGTGACATGGGGCCCTGTG
  CGAAGAGCTGGTTCCTCACAGAGTGGAGTGAAAGGTGCTCAGCGGAGTGTGGGGCAGGAGTGAGGACACG
  TTCAGTGGTGTGCATGACCAACCACGTCAGCAGCCTGCCGTTGGAAGGCTGTGGGAACAACAGGCCAGTG
  GAGGCTACGCCGTGTGACAATGGGCCCTGCACAGGCAAGGTGGAGTGGTTCACTGGCAGCTGGAGTCAGT
  GTTCCATCGAGTGTGGCAGTGGGACACAGCAGAGGGAAGTGATCTGTGTTAGGAAGAATGCAGACACCTT
  TGAAGTGCTAGACCCTTATGAGTGTTCATTCCTGGAGAAGCCCCCAAGCCAGCAGGCCTGCCACCTCAAG
  CCTTGTGGAGCTAAATGGTTTAGCACAGAATGGAGCATGTGCTCCAAGAGCTGTCAGGGCGGATTCCGGG
  TGCGGGAAGTTCGGTGTCTCTCAGATGACATGACCCCAAGTAGCCTCTGCGACCCCCAGTTGAAACCAGA
  AGAGAGAGAGTCTTGTAACACTCAGGACTGTGTCCCTGAAGTTGATGAAAACTGCAAGGACAAGTACTAC
  AACTGCAACGTGGTGGTCCAGGCACGCCTCTGTGTCTACAACTACTACAAGACTGCCTGCTGTGCCTCTT
  GCACCCGAGTGGCTAACAGACACGTGGGCTTTCTGGGGAGCCGATAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001040426
• Insert Size: 3057 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001040426.3, NP_001035516.2
• RefSeq Size: 8796 bp
• RefSeq ORF: 3057 bp
• Locus ID: 207596
• UniProt ID: Q3UTY6
• Cytogenetics: 9 B
• Gene Summary: Promotes FBN1 matrix assembly. Attenuates TGFB signaling, possibly by accelerating the sequestration of large latent complexes of TGFB or active TGFB by FBN1 microfibril assembly, thereby negatively regulating the expression of TGFB regulatory targets, such as POSTN.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (a). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.