Ppargc1b (NM_133249) Mouse Untagged Clone

CAT#: MC223214

Ppargc1b (untagged) - Mouse peroxisome proliferative activated receptor, gamma, coactivator 1 beta (Ppargc1b), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Ppargc1b
• Synonyms: 4631412G21Rik; Perc
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC223214 representing NM_133249
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGGGAACGACTGCGGCGCGCTGCTGGATGAAGAGCTCTCGTCCTTCTTCCTCAACTATCTCTCTG
  ACACGCAGGGTGGGGACTCTGGAGAGGAACAGCTGTGTGCTGACTTGCCAGAGCTTGACCTCTCCCAGCT
  GGACGCCAGTGACTTTGACTCAGCCACGTGCTTTGGGGAGCTGCAGTGGTGCCCGGAGACCTCAGAGACA
  GAGCCCAGCCAGTACAGCCCCGATGACTCCGAGCTCTTCCAGATTGACAGTGAGAATGAAGCTCTCTTGG
  CTGCGCTTACGAAGACCCTGGATGACATCCCCGAAGACGATGTGGGGCTGGCTGCCTTCCCAGAACTGGA
  TGAAGGCGACACACCATCCTGCACCCCAGCCTCACCTGCCCCCTTATCTGCACCCCCCAGCCCCACCCTG
  GAGAGGCTTCTGTCCCCAGCGTCTGACGTGGACGAGCTTTCACTGCTACAGAAGCTCCTCCTGGCCACAT
  CCTCCCCAACAGCAAGCTCTGACGCTCTGAAGGACGGGGCCACCTGGTCCCAGACCAGCCTCAGTTCCAG
  AAGTCAGCGGCCTTGTGTCAAGGTGGATGGCACCCAGGATAAGAAGACCCCCACACTGCGGGCTCAGAGC
  CGGCCTTGTACGGAACTGCATAAGCACCTCACTTCGGTGCTGCCCTGTCCCAGAGTGAAAGCCTGCTCCC
  CAACTCCGCACCCGAGCCCTCGGCTCCTCTCCAAAGAGGAGGAGGAGGAGGTGGGGGAGGATTGCCCAAG
  CCCTTGGCCGACTCCAGCCTCGCCCCAAGACTCCCTAGCACAGGACACGGCCAGCCCCGACAGTGCCCAG
  CCTCCCGAGGAGGATGTGAGGGCCATGGTACAGCTCATTCGCTACATGCATACCTACTGCCTGCCTCAGA
  GGAAGCTGCCCCAACGGGCCCCAGAGCCAATCCCCCAGGCCTGCAGCAGCCTCTCCAGGCAGGTTCAACC
  CCGATCCCGGCATCCCCCCAAAGCCTTCTGGACTGAGTTCTCTATCCTAAGGGAACTTCTGGCCCAAGAT
  ATCCTCTGTGATGTTAGCAAGCCCTACCGCCTGGCCATACCTGTCTATGCTTCCCTCACACCTCAGTCCA
  GGCCCAGGCCCCCCAAGGACAGTCAGGCCTCCCCTGCCCACTCTGCCATGGCAGAAGAGGTGAGAATCAC
  TGCTTCCCCCAAGAGCACCGGGCCTAGACCCAGCCTGCGTCCTCTGAGGCTGGAGGTGAAACGGGATGTT
  AACAAGCCTACAAGGCAAAAGCGGGAGGAAGATGAGGAGGAGGAGGAGGAAGAAGAAGAAGAGGAAGAAG
  AAAAAGAAGAGGAAGAAGAGGAGTGGGGCAGGAAGAGACCAGGTCGTGGCCTGCCATGGACCAAACTAGG
  GAGGAAGATGGACAGCTCCGTGTGCCCCGTGCGGCGCTCCAGGAGACTGAATCCAGAGCTGGGTCCCTGG
  CTGACATTCACTGATGAGCCCTTAGGTGCTCTGCCCTCGATGTGCCTGGATACAGAGACCCACAACCTGG
  AGGAAGACCTGGGCAGCCTCACAGACAGTAGTCAAGGCCGGCAGCTCCCCCAGGGATCCCAGATCCCCGC
  CCTGGAAAGCCCCTGTGAGAGTGGGTGCGGAGACACAGATGAAGATCCAAGCTGCCCACAGCCCACTTCC
  AGAGACTCCTCCAGGTGCCTCATGCTGGCCTTGTCACAAAGCGACTCTCTTGGCAAGAAGAGCTTTGAGG
  AGTCCCTGACGGTGGAGCTTTGCGGCACGGCAGGACTCACGCCACCCACCACACCTCCATACAAGCCAAT
  GGAGGAGGACCCCTTCAAGCCAGACACCAAGCTCAGCCCAGGCCAAGACACAGCTCCCAGCCTTCCCTCC
  CCCGAGGCTCTTCCGCTCACAGCCACCCCAGGAGCTTCCCACAAGCTGCCCAAGAGGCACCCAGAGCGAA
  GCGAGCTCCTGTCCCATTTGCAGCATGCCACAACCCAACCAGTCTCACAGGCTGGCCAGAAGCGCCCCTT
  CTCCTGCTCCTTTGGAGACCACGACTACTGCCAGGTGCTCAGGCCAGAGGCTGCCCTGCAGAGGAAGGTG
  CTGCGGTCCTGGGAGCCAATCGGGGTCCACCTTGAAGACTTGGCCCAGCAGGGTGCCCCTCTGCCAACGG
  AAACAAAGGCCCCTAGGAGGGAGGCAAACCAGAACTGTGACCCTACCCACAAGGACAGCATGCAGCTAAG
  AGACCATGAGATCCGTGCCAGTCTCACAAAGCACTTTGGGCTGCTGGAGACTGCTCTGGAAGGTGAAGAC
  CTGGCGTCCTGTAAAAGCCCGGAGTATGACACCGTATTTGAGGACAGCAGCAGCAGCAGTGGCGAGAGTA
  GCTTCCTGCTTGAGGAGGAGGAGGAAGAGGAGGAGGGAGGGGAAGAGGACGATGAAGGAGAGGACTCAGG
  GGTCAGCCCTCCCTGCTCTGATCACTGCCCCTACCAGAGCCCACCCAGTAAGGCCAGTCGGCAGCTCTGC
  TCCCGAAGCCGCTCCAGTTCCGGCTCCTCGTCCTGCAGCTCCTGGTCACCAGCCACCCGGAAGAACTTCA
  GACGTGAGAGCAGAGGGCCCTGTTCAGATGGAACCCCAAGCGTCCGGCATGCCAGGAAGCGGCGGGAAAA
  GGCCATCGGTGAAGGCCGTGTGGTATACATTCGAAATCTCTCCAGTGACATGAGCTCTCGGGAACTAAAG
  AAGCGCTTTGAGGTGTTCGGTGAGATTGTAGAGTGCCAGGTGCTGACGAGAAGTAAAAGAGGCCAGAAGC
  ACGGTTTTATCACCTTCCGGTGTTCAGAGCACGCTGCCCTGTCCGTGAGGAACGGCGCCACCCTGAGAAA
  GCGCAATGAGCCCTCCTTCCACCTGAGCTATGGAGGGCTCCGGCACTTCCGTTGGCCCAGATACACTGAC
  TATGATCCCACATCTGAGGAGTCCCTTCCCTCATCTGGGAAAAGCAAGTACGAAGCCATGGATTTTGACA
  GCTTACTGAAAGAGGCCCAGCAGAGCCTGCATTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_133249
• Insert Size: 3045 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_133249.2, NP_573512.1
• RefSeq Size: 3656 bp
• RefSeq ORF: 3045 bp
• Locus ID: 170826
• UniProt ID: Q8VHJ7
• Cytogenetics: 18 E1
• Gene Summary: Plays a role of stimulator of transcription factors and nuclear receptors activities. Activates transcriptional activity of estrogen receptor alpha, nuclear respiratory factor 1 (NRF1) and glucocorticoid receptor in the presence of glucocorticoids. May play a role in constitutive non-adrenergic-mediated mitochondrial biogenesis as suggested by increased basal oxygen consumption and mitochondrial number when overexpressed. May be part of the pathways regulating the elevation of gluconeogenesis, beta-oxidation of fatty acids and ketogenesis during fasting. Stimulates SREBP-mediated lipogenic gene expression in the liver. Induces energy expenditure and antagonizes obesity when overexpressed. Induces also the expression of mitochondrial genes involved in oxidative metabolism. Induces the expression of PERM1 in the skeletal muscle in an ESRRA-dependent manner.[UniProtKB/Swiss-Prot Function]