Hira (NM_010435) Mouse Untagged Clone

CAT#: MC223203

Hira (untagged) - Mouse histone cell cycle regulation defective homolog A (S. cerevisiae) (Hira), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Hira
• Synonyms: AA138857; D16Ertd95e; Gm15797; N28177; OTTMUSG00000026264; Tuple1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC223203 representing NM_010435
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGCTCTTGAAGCCAACCTGGGTCAACCACAATGGGAAGCCAATTTTTTCAGTTGATATTCACCCTG
  ATGGGACCAAGTTTGCAACTGGAGGACAAGGGCAGGATTCTGGGAAGGTTGTGATCTGGAATATGTCTCC
  AGTCCTCCAGGAGGATGACGAGAAGGATGAAAATATTCCCAAGATGCTTTGCCAGATGGACAATCACTTA
  GCATGTGTGAACTGTGTGCGGTGGTCAAACAGTGGGATGTATTTAGCTTCTGGGGGAGATGACAAACTGA
  TTATGGTGTGGAAGCGGGCTACGTACATTGGGCCCAGCACTGTGTTTGGTTCCAGTGGTAAGCTTGCCAA
  TGTGGAGCAATGGCGGTGTGTCTCCATCCTCCGGAGTCACTCAGGCGATGTGATGGATGTAGCATGGTCT
  CCCCACGACGCCTGGCTGGCCTCATGCAGCGTGGATAACACTGTTGTCATTTGGAATGCCGTGAAGTTCC
  CAGAAATTCTTGCAACTCTGAGAGGTCATTCTGGCTTAGTAAAAGGTTTGACTTGGGATCCCGTTGGTAA
  ATATATTGCCTCTCAAGCTGATGATCGAAGTTTGAAGGTATGGAGGACGCTGGACTGGCAGCTAGAGACT
  AGCATCACCAAGCCTTTTGATGAGTGTGGAGGAACGACGCATGTTCTCCGGCTTAGTTGGTCACCTGATG
  GCCATTACCTGGTATCTGCCCATGCCATGAATAATTCTGGCCCCACTGCTCAGATCATCGAAAGAGAGGG
  CTGGAAGACCAACATGGACTTTGTGGGTCACCGGAAAGCTGTGACTGTTGTGAAATTCAACCCAAAAATC
  TTCAAGAAGAAGCAGAAGAATGGGAGCTCTACAAAGCCCAGCTGCCCATACTGCTGCTGTGCTGTTGGCA
  GCAAGGACCGCTCACTCTCTGTCTGGCTCACATGTTTGAAACGGCCTCTGGTTGTCATCCATGAACTGTT
  TGACAAGTCCATCATGGATATTTCCTGGACTCTGAATGGGTTGGGTATCCTGGTGTGCTCCATGGACGGC
  TCTGTGGCGTTCCTTGATTTCTCTCAGGATGAACTCGGAGACCCCCTGAGTGAGGAGGAAAAGAGCCGAA
  TTCACCAGTCTACCTATGGCAAGAGCCTGGCAATAATGACTGAGGCCCAGCTTTCCACAGCTGTTATTGA
  GAACCCTGAGATGCTCAAGTACCAGCGGAGACAGCAACAGCAGCAGCTGGATCAGAAGAATGCCACTACT
  AGGGAGACAAGCTCAGCATCCTCAGTCACGGGTGTGGTCAATGGGGAAAGTCTAGAAGATATCAGAAAGA
  ATCTTTTGAAGAAACAAGTGGAAACTCGGACAGCAGATGGTCGGAGGAGAATCACGCCTCTTTGCATAGC
  ACAGCTGGACACTGGGGACTTCTCCACGGCATTCTTCAACAGCATCCCACTCTCCAGCTCCCTAGCAGGC
  ACCATGCTCTCCTCTCCTAGTGGTCAGCAGCTACTACCACTGGACTCCAGTACCCCCTCCTTCGGCGCCT
  CAAAGCCTTGCACAGAACCAGTGGCAGCCACCAGTGCCAGGCCTACAGGCGAATCTGTCAGTAAGGACAG
  TATGAATGCTACCTCTACTCCTGCTGCATCGTCACCCTCTGTGTTAACAACCCCATCCAAGATTGAACCC
  ATGAAAGCATTTGATTCCCGGTTCACAGAACGGTCCAAAGCCACACCAGGTGCTCCTTCCTTGACCAGTG
  TGATTCCAACAGCTGTTGAAAGGTTGAAAGAGCAGAACCTCGTCAAGGAGCTGAGGTCCCGGGAACTGGA
  GAGCAGCAGTGACAGCGATGAGAAGGTCCACCTAGCCAAGCCCTCTTCACTGTCCAAGCGCAAACTTGAG
  CTTGAGGTAGAGACGGTGGAAAAGAAGAAGAAAGGTCGCCCTAGGAAGGATTCACGTCTTTTACCCATGT
  CTCTGTCCGTCCAGTCTCCAGCTGCCCTGTCTACAGAGAAGGAGGCCATGTGTCTGTCTGCACCAGCACT
  TGCACTGAAGCTGCCAATTCCAGGCCCACAGAGAGCGTTCACCCTCCAGGTGAGCTCTGACCCCTCCATG
  TACATTGAGGTGGAGAATGAAGTGACCACCGTTGGGGGGATAAGGCTGAGTCGCCTGAAGTGCAACCGTG
  AAGGGAAGGAGTGGGAGACAGTGCTCAGCAGTCGCGTCCTCACTGCTGCCGGCAGCTGTGATGTGGTATG
  TGTTGCCTGTGAAAAGAGGATGCTGTCGGTGTTCTCTACCTGTGGTCGCCGTCTCCTCCCTCCCATCCTC
  CTTCCATCTCCAATCTCTACTTTGCACTGCACGGGCCCCTACGTCATGGCACTCACCGCTGCAGCCACAC
  TGTCTGTCTGGGATGTTCACAGACAGGTGGTTGTGGTGAAAGAAGAATCTCTACACTCCATCTTGTCAGG
  AAGTGATATGACGGTGTCACAGATCTTGCTAACACAGCATGGAATCCCAGTGATGAATTTGTCTGATGGG
  AAAGCATACTGCTTTAATCCATCCCTCTCCACATGGAACCTGGTTTCTGACAAGCAAGATTCATTGGCCC
  AGTGTGCAGACTTCAGGAACAGCCTGCCGTCCCAGGATGCCATGCTTTGTTCAGGACCGTTAGCCATAAT
  TCAGGGCCGCACCTCCAACTCTGGAAGGCAAGCTGCCCGGCTCTTCTCCGTGCCTCATGTGGTACAGCAG
  GAGACCACACTGGCCTACCTAGAGAACCAGGTTGCTGCGGCACTTACCCTGCAGTCAAGCCACGAGTATC
  GTCACTGGCTCCTCCTTTATGCTCGGTACCTTGTGAATGAAGGGTTTGAATACCGCCTCCGTGAAATATG
  CAAGGACTTGCTGGGTCCAGTTCACTGCTCCACTGGAAGTCAGTGGGAGTCAACAGTAGTGGGTCTGCGG
  AAAAGGGAGCTGCTGAAGGAACTGTTGCCAGTCATTGGGCAGAATCTTCGATTCCAGCGCCTCTTTACTG
  AGTGCCAGGAACAACTGGACATCCTGAGAGACAAGTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_010435
• Insert Size: 3048 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_010435.2, NP_034565.2
• RefSeq Size: 4553 bp
• RefSeq ORF: 3048 bp
• Locus ID: 15260
• UniProt ID: Q61666
• Cytogenetics: 16 11.69 cM
• Gene Summary: Required for the periodic repression of histone gene transcription during the cell cycle (By similarity). Cooperates with ASF1A to promote replication-independent chromatin assembly. Required for the formation of senescence-associated heterochromatin foci (SAHF) and efficient senescence-associated cell cycle exit.[UniProtKB/Swiss-Prot Function]