Dennd2a (NM_172477) Mouse Untagged Clone

CAT#: MC223156

Dennd2a (untagged) - Mouse DENN/MADD domain containing 2A (Dennd2a), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Dennd2a
• Synonyms: B930096L08Rik; C130031G22; mKIAA1277
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC223156 representing NM_172477
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTGGAGGCCAGAGTGGACATGCTCAGCTCCAACATGATCATCAGTGGCCCGGCTGCAGACCTTGGCG
  CTAAAGAAGCCAGCAGACCCTGGAAGAAGCAGCTTAACAGTGTCCCGAACTCTGGACCATCCGCCCGAGC
  CAGAGCCCAGCCCCAGCCCCTCAGCATAAAGGACAAGATATCCAAGTGGGAAGGCAAGAAGGAGCCACCC
  GCCTCCGATCCCGCCAGGCAAACAGATGGCCAAGAGGACCACCTGCCATCCTGCAAGGTAGAGAGAAGAG
  GCAGTGAGCTCACCAGAACTAAGAATGGAATGAGGCTTGAAACAGAGAGATTGCAAAATGACAGCCGGGC
  AAGAACTGTGTGTCAGGACACAGAACAGTTGCCAGGCCCGAGGCCTATCGATGGACAGCCAGAGCTCAGC
  CAGCACCGAGGTCGTGAGCTGAAACCCAGCGATCTACGCTTTCAAAGTGACCATCTCTCTGTGCTGAGAC
  AGGTCAAGAGACTAGAGAAGGCCTTGAAGGATGGCTCCGCTGGCTTGGACCCTCAGATGCCGGGGACTTG
  TTATTCACCTCACTGTCTGCCTGACAAAACAGAGGAAGACCTGCCCAGTCTTGAAAGCCACGAGAAGGGG
  GGTGTCTTAGCAGCTGGCCGGAGAGCTCACCACCTGGAGGTCAGGGAGCCGGGCCCTGAGATTTCAGAGG
  ATTGGAAAGGCCAGGAGAGTGTCTATAGGGGCTCCAGGTGGTACCCCCCAAAGCCTTTTATCAACCCTGT
  CCCCAAGCCCCGCAGAACATTCAAACATGCTGGAGAAGGGGACAAGGATGTCAGCCCAGGCATCAGCTTC
  AAGAAAGAGAAGAGAAACCTGCCTCCCCTGCCCTCTCTGCCTCCCCCTCCCCCACCGCTGCCTTCCTCCC
  CTCCTCCCACTTCCGTGAATAGAAGACTGTGGACCGGGAGGCAGAGACCCAGTGCTGACCACAGAAAGTC
  CTATGAGTTCGAGGACTTACTACAGTCTTCCTCAGAGAACAGCAGGGTGGACTGGTATGCACAGACCAAA
  TTGGGACTCACACGCACTTTATCGGAGGAGAACGTCTATGAAGACATTCTAGATCCGCCAATGAAGGAAA
  ATCCTTATGAAGATGTGGAGCTGCATGGCCGCTGCCTGGGCAAGAAGTGTGTCTTGACTTTCCCCGCATC
  TCCCACCTCTTCCATCCCGGACACGTCCACCAAGCAGTCCTTGTCCAAGTCTGCATTTTTCCGGCAGAAC
  TCAGAAAGAAGAAACTTGAAGCTGTTGGACACTAGGAAGCTTAGTCGGGATGGAGCTGGGTCCCCGCTGA
  GGACCAGTCCTCCCTCTACACCTAGCAGCCCGGACGACACGTTCTTTAACCTTGGAGACCTACAGAATGG
  CAGGAAGAAGAAAAAGATCCCCAGGCTGGTATTGAGGATCAATGCCATTTATGAGGCCCGGAGAGGAAAG
  AAACGGGTGAAGAGGCTATCCCAGTCCACGGAGAGCAACTCAGGAAAAGTGACAGATGAGAACAGTGAGT
  CTGACAGCGACACAGAGGAGAAGCTGAAAGCTCACAGCCAGCGCCTGGTCAATGTGAAGTCTCGCCTGAA
  GCAGGCCCCTCGATACTCATCGCTTGACCGGGATCTCATTGAGTACCAAGAGAGGCAACTCTTCGAGTAC
  TTCGTGGTTGTGTCTCTGCACAAGAAGCAGGCAGGGGCCGCCTACGTGCCAGAGCTCACCCAGCAATTTC
  CTCTGAAGTTAGAAAAGTCATTCAAGTTCATGAGAGAGGCTGAGGACCAGCTGAAGGCTATCCCCCAGTT
  CTGCTTCCCCGATGCTAAGGACTGGGCTCCTGTCCAGGAGTTTACCAGTGAAACATTCTCGTTTGTCTTA
  ACTGGAGAAGACGGGAGCAGAAGGTTCGGTTACTGCCGAAGACTGCTGCCTGGGGGCAAAGGGAAGCGGC
  TTCCTGAGGTTTATTGCATTGTGAGCCGCCTGGGATGCTTCAGCCTCTTTTCAAAGATCTTGGATGAGGT
  GGAAAAGAGGAGAGGAATCTCCCCTGCCCTCGTGCAGCCCCTCATGAGGAGCGTCATGGAAGCCCCTTTC
  CCAGCCCTGGGCAAAACCATCATTGTCAAGAACTTCCTGCCAGGCTCGGGGACTGAGGTGATTGAGCTGT
  GCCGACCGCTGGACTCCCGGCTAGAGCACGTGGACTTTGAGTCTCTGTTCTCCTCCCTCAGTGTCCGACA
  CTTGGTCAGTGTCTTTGCCTCCCTCCTTCTGGAAAGGAGAGTCATCTTCATCGCAGACAAACTCAGCACT
  CTGTCCAAATGCTGCCACGCAATGGTGGCGCTCATCTACCCCTTCAGCTGGCAGCACACCTACATCCCGG
  TGCTACCGCCCGCCATGATCGACATAGTGTGCTCGCCCACGCCCTTCCTCATCGGGCTGCTCTCCAGCTC
  GCTGCCTCTGCTCAGGGAGCTGCCACTGGAAGAGGTTCTCGTGGTCGATCTCATCAATGATCGGTTCCTC
  AGGCAGATGGAGGACGAGGATTCCATTCTGCCCCGGAAGCTTCAGGTTGCCCTGGAACACATTCTGGAGC
  AGAGGAATGACCTGGCCTGTGACCAGGATGGAGGCCCGCTGGACTGTGTGCACGGCCCTGAATCCAGTTC
  CTTGAGTGAGGTGGTGTCCGAAGCCTTTGTCCGCTTTTTTGTGGAGATTGTGGGCCACTACCCCCTGTTC
  CTGACATCGGGTGAGGAGAGAAGCCTGCAACGAGAGGCCTTCAGAAAAGCTGTCTCCTCTAAGAGCCTGC
  GGCGCTTCCTGGAGGTCTTCATGGAGACCCAGACGTTCCGTGGCTTCATCCAGGAGCGGGAACTACGCAG
  GCAAGATGCCAAAGGTCTGTTTGAAGTCCGAGCCCAGGAGTACCTGGAAACCCTGCCCAGCGGAGAGCAC
  AGCGGAGTCAATAAATTCCTCAAGGGACTGGGCAATAAAATGAAGTTTCTCCACAAGAAATAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_172477
• Insert Size: 3003 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_172477.4, NP_766065.1
• RefSeq Size: 4435 bp
• RefSeq ORF: 3003 bp
• Locus ID: 209773
• UniProt ID: Q8C4S8
• Cytogenetics: 6 B1
• Gene Summary: Guanine nucleotide exchange factor (GEF) which may activate RAB9A and RAB9B. Promotes the exchange of GDP to GTP, converting inactive GDP-bound Rab proteins into their active GTP-bound form. May play a role in late endosomes back to trans-Golgi network/TGN transport (By similarity).[UniProtKB/Swiss-Prot Function]