Phf20 (BC060121) Mouse Untagged Clone

CAT#: MC223127

Phf20 (untagged) - Mouse PHD finger protein 20 (cDNA clone MGC:76344 IMAGE:6413021), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Phf20
• Synonyms: 6820402O20Rik
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC060121
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACCAAGCACCCACCTAACAGACGAGGAATCAGCTTTGAAGTGGGAGCCCAGTTGGAAGCTCGGGACC
  GTTTAAAAAACTGGTATCCAGCTCATATTGAAGACATTGACTACGAAGAAGGGAGAGTTCTCATCCATTT
  CAAGCGTTGGAACCATCGTTACGATGAGTGGTTTTGCTGGGACAGTCCTTATCTGCGCCCTTTAGAGAAG
  ATCCAGCTGAGGAAAGAGGGTTTACATGATGAGGATGGCTCTTCTGAATTTCAAATAAACCAGCAAGTGC
  TTGCTTGCTGGTCTGACTGTCGATTTTATCCAGCCAGAGTCACTGCTGTGAACAAGGATGGTACTTACAC
  TGTGAAATTTTATGATGGAGTAGTTCAAACTGTCAAACATATTCATGTCAAAGCTTTTTCCAAAGATCAG
  AATATTGTGGGTAATGCTAGGCCTAAAGAAACAGACCACAAAAGTCTTTCATCGTCTCCTGAGAAACGAG
  AGAAGTTTAAAGAACAGAGAAAAGTCACGGTCAATGTGAAGAAAGACAAAGTGGAAAAAGCCTTAAAGAC
  AGAAAAGCGGCCCAAGCAACCTGACAAAGAGGGAAAGCTGATCTGCTCAGAAAAAGGCAAAGTGTCAGAG
  AAAAGCCTTCCTAAGAACGAAAAGGAAGATAAGGAGAACATTTCCGAGAACGAGCGGGAGTACTCCGGGG
  ATGCCCAGGTGGAAAAGAAGCCTGAGAAGGACCTTGTGAAGAACCCACAAGAGAACCTGAAGGAGCCAAA
  AAGAAAACGAGGCAGACCCCCTTCCATAACTCCTACGGCTGTGGATTCAAACTCTCAAACTTTGCAACCA
  ATAACATTGGAATTGAGAAGACGGAAAATATCAAAACGAAGTGACACCCCATTAAAGCGTCCCAGACTCG
  ACAAAAATTCACCCCAGGAACAGTCAAAAAAACGCTCTGAAAATAGTGACAAAGACTTATCCAGGAGACG
  GTCCTCCAGGCTGTCCACTAATGGGACCCGTGAGATCCTAGATCCTGACTCGATTGTACCTGATCTGGTT
  CATACGGTTGATACAAACCCTCTACCAGACAAGTCACCCAGTGCCAAGGATTCTGCTGAAGGTCAGTTGA
  AGTCTCCATTGGAAGCTGGCCAGGTCTCTTCTGCATTAACTTGCCACCCCATTGGGGATGGCCTGGGGGC
  AGCAGATTTGGAGTTGAATTGCAAGTCAATGGGAGAAAACACGATGAAAACAGAACCTGTTTCTCCTCTT
  GCTGAGGTGCAGGAAGTTTCAACTGTTGAAGTTCCAAATACTTTGAAGAAAGTTGATGACTCTGTGACGT
  TGAATGTGCCAGCTGTGGACCTAGACCACAAGTTTCGATGCAAGGTTCTGGACTGTTTGAAATTTTTCCG
  CAAGGCTAAATTGCTGCACTATCATATGAAGTATTTCCATGGGATGGAGAAGTCACCAGAGCCAGAGGAG
  GGCCCAGGGAAGACGCATGTACAGACTCGGGGCTCTGCGGTGCCTGACAAGACAAGCCAGGAGAGCCTAA
  CCAGGAAGCGGGTCTCTGCCAGTTCCCCCACTGCAAAAGAGAAGGAAAAGACTAAAGAGAAGAAATTCAA
  AGAACTTGTGAGAGTGAAGCCAAAGAAGAAGAAATTCAAAGAACTTGTGAGAGTGAAGCCAAAGAAGAAA
  AAGAAAAAGAAAAAGAAAACCAAGCCTGAATGTCCCTGCAGTGAGGACATCAGTGATACTTCCCAGGAAC
  CTTCTCCACCCAAAACATTTGCTGTCACCAGGTGTGGGTCCTCACACAAGCCTGGGGTCCATATGAGCCC
  GCAGCTCCATGGTTCAGATAATGGAAACCACAAAGGGAAATTGAAAACCTGTGAGGAGGATAATTTGAGT
  GAGTCCTCTTCCGAGAGCTTTCTTTGGAGTGATGAGGAATATGGTCAAGATGTTGATGTGACCACCAACC
  CAGATGAGGAGCTTGAGGGCGACGACCGCTATGATTTTGAGGTGGTCCGCTGCATCTGTGAAGTGCAGGA
  GGAAAATGACTTCATGATTCAGTGTGAAGAGTGCCAGTGCTGGCAGCACGGGGTCTGCATGGGCTTACTG
  GAAGAAAACGTGCCTGAGAAATACACCTGCTATGTTTGCCAAGACCCTCCAGGTCAGAGGCCTGGCTTCA
  AGTACTGGTATGACAAGGAGTGGCTGAGCCGGGGACACATGCATGGTCTGGCATTTCTGGATCAGAACTA
  CTCCCACCAGAATGCCAGGAAGATCGTGGCCACCCACCAGCTGCTCGGGGACGTGCAGAGAGTGATCCAA
  GTGCTGCACGGCCTGCAGCTCAAGATGAGCATTCTGCAAAGCAGAGAGCATCCTGATCTGCAGCTATGGT
  GCCAGCCCTGGAAACAGCACTCGGGGGAGGGAAGAGCACATCCCAGACACATCCACATCACAGACGCCAG
  GAGCGAGGAGTCCCCAAGCTATAGAACTTTGAATGGGGCGGTGGAGAAGCCATCGCCCCTGCCCCGATCT
  GTGGAGGAGTCTTACATCACCAGTGAGCATTGCTACCAGAAACCTCGCGCCTATTATCCCGCTGTGGAGC
  AGAGGCTGGTCGTGGAGACGAGAGGCTCTGCCCTTGATGCTGCAGTCAGCCCCCTCTGTGAGAACGGCGA
  CGACTCCCTCTCCCCTCGCCTAGGCTGGCCCATCGACCAAGACAGGAGCAGAGGCGACATAGATCCCAAA
  CCCAGCTCCCCAAAGGTGAGAGAGTACATTTCCAAGAATGTCTTGCCAGAAGAGACGCCTGCAAGGAAGC
  TGCTGGACAGAGGTGGAGAAGGGCTGGTGAGCTCTCAGCACCAGTGGCAGTTCAACCTGCTCACACATGT
  GGAGTCCCTGCAGGATGAGGTGACGCACCGGATGGACTCCATTGAGAAGGAGCTGGACGTGCTGGAGAGC
  TGGCTGGACTACACTGGGGAGCTGGAGCCCCCAGAGCCACTGGCCAGGCTTCCGCAGCTCAAGCACTGCA
  TCAAGCAGCTGCTGACTGACCTGGGCAAGGTGCAGCAGATCGCCCTCTGCTGCTCGACATGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCTGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: BC060121
• Insert Size: 3072 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC060121, AAH60121
• RefSeq Size: 5665 bp
• RefSeq ORF: 3071 bp
• Locus ID: 228829
• Cytogenetics: 2 H1
• Gene Summary: Contributes to methyllysine-dependent p53/TP53 stabilization and up-regulation after DNA damage (By similarity). Methyllysine-binding protein, component of the MOF histone acetyltransferase protein complex. Not required for maintaining the global histone H4 'Lys-16' acetylation (H4K16ac) levels or locus specific histone acetylation, but instead works downstream in transcriptional regulation of MOF target genes. As part of the NSL complex it may be involved in acetylation of nucleosomal histone H4 on several lysine residues.[UniProtKB/Swiss-Prot Function]