BC033915 (BC063268) Mouse Untagged Clone

CAT#: MC223095

BC033915 (untagged) - Mouse cDNA sequence BC033915 (cDNA clone MGC:68087 IMAGE:4187769), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: BC033915
• Synonyms: 5730525O22Rik; 9030204A07Rik; AI447252; AI846254; AI849445; mKIAA0999; Qsk; SIK-3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC063268
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCGCTCGCATCGGCTACTACGAGATCGACCGCACCATCGGCAAGGGCAACTTCGCTGTGGTCAAGC
  GGGCCACGCACCTCGTCACCAAGGCCAAGGTTGCTATTAAAATCATAGATAAGAGCCAGCTGGATGAAGA
  AAACTTGAAGAAGATTTTCCGGGAGGTTCAGATAATGAAGATGCTTTGCCATCCACACATCATCAGACTC
  TACCAGGTCATGGAGACAGAGCGCATGATTTACCTGGTGACAGAATACGCTAGCGGAGGGGAGATATTTG
  ACCACTTGGTAGCCCATGGAAGAATGGCAGAGAAGGAAGCTCGACGGAAGTTCAAACAGATCGTCACAGC
  GGTGTATTTTTGTCACTGTCGGAATATCGTTCATCGTGATTTAAAAGCCGAAAACTTACTTCTGGACGCC
  AATCTGAATATCAAAATAGCAGACTTTGGCTTCAGCAACCTCTTCACTCCAGGGCAGCTGCTGAAGACGT
  GGTGTGGCAGCCCTCCCTATGCCGCCCCAGAGCTCTTCGAAGGGAAGGAATATGATGGGCCCAAAGTGGA
  CATATGGAGCCTTGGAGTTGTCCTCTATGTGCTGGTGTGTGGCGCCCTGCCGTTTGATGGGAGCACACTG
  CAGAATCTGCGGGCCCGCGTGCTGAGTGGCAAGTTCCGCATCCCGTTCTTTATGTCCACAGAGTGTGAGC
  ACTTGATCCGCCACATGCTGGTGTTAGATCCAAATAAGCGCCTCTCAATGGAACAGATCTGCAGGCACAA
  GTGGATGAAGCTCGGAGATGCAGACCCCAACTTTGACAGGTTAATAGCGGAGTGCCAGCAACTGAAGGAG
  GAAAGGCAGTCGGACCCCCTCAACGATGATGTCCTCTTGGCTATGGAAGACATGGGGCTGGACAAGGAGC
  GCACACTTCAGTCATTAAGGTCAGATGCCTATGACCACTATAGTGCAATCTACAGCCTGCTGTGCGATCG
  ACACAAGAAACATAAAACTCTGCGTCCCGGAGCACTTCCCAGCATGCCCCAAGCCATGACTTTCCAGGCA
  CCAGTCAATCTCCAGGCGGAGCAGACAGGCACTGCTATGAACCTTAGCGTCCCTCAAGTTCAGCTGATCA
  ACCCAGAGAACCAAATTATAGAGCCTGACGGGGCTGTGAACTTGGACAGTGATGAGGGTGAAGAGCCTTC
  TCCAGAAGCCTTGGTTCGCTATTTGTCAATGAGGAGGCACACGGTGGGAGTGGCTGACCCACGCACGGAA
  GTTATGGAAGATCTGCAGAAGCTGCTGCCTGGCTTTCCTGGAGTAAACCCACAGGGTCCATTCCTACAAG
  TGGCCCCTAACATGAACTTCACGCACAACCTGTTGCCCATGCAGAGTCTGCAGCCAACTGGGCAGCTTGA
  GTACAAGGAACAGTCTCTGTTACAGCCGCCTACACTACAGCTACTGAACGGAATGGGGCCCCTTGGCCGG
  AGAGCCTCAGATGGAGGCGCCAACATCCAACTGCATGCCCAGCAGCTGCTCAAGCGCCCACGGGGACCGT
  CCCCACTTGTCACCATGACACCAGCAGTGCCAGCAGTTACCCCTGTGGATGAGGAGAGCTCGGATGGGGA
  GCCAGATCAGGAAGCTGTGCAGAGGTACTTGGCAAATAGGTCCAAAAGGCACACACTGGCCATGACCAGC
  CCGACAGCTGAGATCCCACCGGACCTGCAACGGCAGCTAGGACAACAGTCTTTCCGTTCCCGGGTCTGGC
  CTCCTCACCTGGTACCTGACCAGCATCGCTCCACCTACAAGGACTCCAACACCCTGCATCTCCCTACGGA
  GCGTTTCTCCCCCGTGCGCCGGTTCTCAGACGGGGCTGCAAGCATCCAGGCCTTCAAAGCTCACCTGGAA
  AAAATGGGCAACAGCAGCAGCATCAAGCAGTTGCAGCAGGAGTGTGAGCAGCTGCAGAAGATGTACGGGG
  GGCAGGTGGATGAGAGGACCCTGGAGAAGACCCAGCAGCAGCATATGTTGTACCAGCAGGAGCAGCACCA
  TCAGATCCTCCAGCAGCAAATCCAAGATTCTATTTGTCCTCCTCAGCCCTCCCCACCTCTTCAGGTTGCC
  TGTGAAAACCAACCAGCCCTCCTCACCCACCAGCTCCAGAGGTTAAGGATTCAACCTTCAAGCCCACCCC
  CCAACCATCCCAGCAACCATCTCTTCAGACAGCCAAGTAACAGTCCTCCCCCGGTCAGCAGTGCCATGAT
  CACGTCTCACGGTGCTACGTCTCCCTCCCAGTTTCAAGGCTTACCCTCCCATGGTGCAATCTTCCAGCAG
  CAACCCGAGAACTGTTCCCCGCCTCCCAGTGTAGCACTAACCTGCCTGGGCCTACAGCAGGCTAGCCAGT
  CACAGCCAGTGACTATCCAGCTACAGGAGCCAGTTGACATGCTCAGCAACATGGCCGGAACCGCGGCAGG
  CTCTGCAGGGCGGAGCATCCCCATCAGCCCCAGCGCCAGTCAGATTCAGATACAGCACCGAGCCAGCCTA
  ATGGCTCCCTTCAGCTATGGGCACCGGCCCTTGTCCAAGCAGCTGAGCGCTGACAGTGCAGAGGCCCACA
  GCTTGAACATGAATCGGTTCTCCCCTGCCAACTACGACCAGGCGCATTTACACCCCCATCTGTTTTCGGA
  CCAGTCCCGAGGTTCCCCCAGCAGCTACAGCCCTTCAACAGGAGTGGGGTTTCCTCCAACTCAAGCCCTG
  AAAGTTCCTCCGCTTGACCAGTTCCCCACTTTCCCTCCCAGTGCCCAGCAGCAGCCACCCCACTATACCA
  CGTCAGCACTACAGCAGGCCCTGCTATCTCCTACACCGCCAGACTATCCCCGACACCAGCAGGTTCCCCA
  TATCCTTCAAGGACTGCTCTCTCCCCGGCATTCACTCACCGGCCACTCGGACATTCGGCTGCCTCCGGCA
  GAGTTTGCACAGCTCATCAAAAGGCAGCAACAGCATCGACAGCAGCAGCAGCAGCAGCAGCAGCAACAAG
  AATACCATGAATTGTTCAGGCACATGAACCAAGGGGATGCTGTTAGCCTAGCTCCCAGCCTCGGGGGACA
  GAATATGACAGAGCACCAGGCTTTATCTTATCAAAATGCTGACTCGTACCACCGCCACCACACCAGCCCC
  CAGCATATCTTACAGATCAGGGCGCAAGATTGTATCTCACAGGGTCCCTCGCCCACCCCCACCCATGGCT
  ATGCCCATCAGCCACCACTAATGCATTCGGAGAGTATGGAGGAAGACTGCTTGTGTGAGGGGCTCAAGGA
  GGGCTTCCCAGACAAGAGCTCAAGCACACTGACCAAAGGTTGCCACAACAGCCCTCTGCTCTTGTGTACC
  AGTGGGCCTGGGGACCCTGAGCCTTTGCTGGGAACTGTGAGTCAGGCCCGGGAGCTGGGGATCCATCCCT
  ACGGACACCAGCCAACTGCCACCACGTTCAGTAGAAATAAGGTGCCCAGCCGAGAGTCTGTCCTAGGGAA
  CTGCCTGGAAAGAAGTTCTCCTGGACAAGCAATGGAGCTGCCGGATCACAACGGCCTTGGGTACCCAGTA
  CGGCCCTTAGTCAGTGAGCACCTCAGGTCCCGGACGCTCCAGAGACACCACACGATCCAGAACAGCGACG
  ATGCCTATGTACAGCTGGATACCTTGCCAGGAATGAGCCTGGTGGCAGGCAAGGCGCTGAGCTCTGCCCG
  GATGTCAGATGCAGTTCTTAGTCAGTCTTCACTCATGGGCAGCCAACAGTTTCAGGATGAGGAAGATGAA
  GAATGTGGGGTGAGCCTGGGCCACGAGCATCCAGGCCTGGGTGATGGCAACCAGCATCTCAACTCCTCTC
  GCTATCCAGCTACGTGTGTTACAGACATCATGCTCAGCCACAAGCACCCAGAGGTCTCCTTCAGCATGGA
  GCAAGCCGGCGTGTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCTGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: BC063268
• Insert Size: 3936 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC063268, AAH63268
• RefSeq Size: 6099 bp
• RefSeq ORF: 3935 bp
• Locus ID: 70661
• Cytogenetics: 9 A5.2
• Gene Summary: Positive regulator of mTOR signaling that functions by triggering the degradation of DEPTOR, an mTOR inhibitor (By similarity). Required for chondrocyte hypertrophy during skeletogenesis (PubMed:22318228). Negatively regulates cAMP signaling pathway possibly by acting on CRTC2/TORC2 and CRTC3/TORC3 (By similarity). Prevents HDAC4 translocation to the nucleus (PubMed:22318228).[UniProtKB/Swiss-Prot Function]