Cit (BC023775) Mouse Untagged Clone

CAT#: MC223050

Cit (untagged) - Mouse citron (cDNA clone MGC:38411 IMAGE:5346007), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Cit
• Synonyms: CRIK, CRIK-SK, citron-N, Cit-k
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC023775
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGGCTCTGAAGACGACATGCACCATGTTGGAGGAGCAGGTCCTGGACCTGGAGGCCCTGAACGACG
  AGCTGCTGGAGAAGGAGCGCCAGTGGGAGGCCTGGCGGAGCGTCCTTGGCGACGAGAAGTCCCAGTTCGA
  GTGTCGAGTTCGAGAGCTACAGAGGATGCTGGACACCGAGAAGCAGAGCAGGGCTAGGGCCGATCAGCGG
  ATCACCGAGTCGCGCCAGGTGGTGGAGTTGGCGGTGAAGGAACACAAGGCCGAGATTCTTGCTCTGCAGC
  AGGCTCTCAAGGAGCAGAAGCTCAAAGCCGAGAGCCTGTCGGACAAGCTCAACGACCTGGAGAAGAAGCA
  CGCCATGCTGGAGATGAACGCCCGGAGCTTACAGCAGAAACTAGAGACAGAGCGGGAGCTCAAACAGAGG
  CTTCTGGAGGAGCAAGCCAAATTGCAGCAGCAGATGGACCTGCAGAAGAACCACATCTTCAGACTGACGC
  AAGGGCTGCAGGAGGCGCTGGACCGGGCGGATCTGCTGAAGACAGAAAGGAGCGACCTGGAGTACCAGCT
  GGAAAACATTCAGGTTCTCTACTCTCACGAGAAAGTGAAAATGGAAGGCACAATCTCTCAGCAAACCAAA
  CTCATTGATTTCCTGCAAGCCAAAATGGACCAGCCTGCTAAAAAGAAAAAGGGTTTATTTAGTCGACGGA
  AAGAGGACCCTGCTTTGCCCACACAGGTTCCTCTGCAGTACAATGAGCTGAAGCTAGCCCTGGAAAAGGA
  GAAAGCCCGATGCGCGGAGCTGGAGGAGGCCCTTCAGAAGACCCGCATCGAACTCCGCTCTGCGCGGGAG
  GAAGCTGCCCACCGCAAAGCCACGGACCACCCGCACCCGTCTACGCCAGCCACTGCGAGGCAGCAGATCG
  CCATGTCTGCCATTGTGCGGTCGCCCGAGCACCAGCCCAGTGCCATGAGCCTGCTTGCCCCACCATCCAG
  CCGCAGAAAGGAGTCGTCAACTCCAGAGGAATTCAGCCGGCGTCTGAAAGAGCGCATGCACCACAACATC
  CCTCACCGGTTTAATGTGGGCCTGAACATGAGAGCCACCAAGTGCGCCGTGTGTCTGGATACTGTGCACT
  TTGGACGCCAGGCATCCAAATGCCTCGAATGTCAAGTGATGTGTCACCCCAAATGCTCCACCTGCTTGCC
  CGCTACCTGTGGCCTGCCAGCTGAATATGCCACACACTTCACTGAGGCCTTCTGCCGGGACAAAATGAAC
  TCCCCGGGGCTCCAGAGCAAGGAGCCTGGCAGCAGCTTGCACCTGGAAGGGTGGATGAAAGTGCCCAGAA
  ATAACAAACGGGGACAGCAAGGCTGGGACAGGAAGTACATTGTTCTGGAGGGGTCAAAAGTCCTCATCTA
  TGACAATGAAGCCAGAGAAGCTGGACAGAGGCCGGTGGAAGAATTTGAGCTGTGCCTTCCTGACGGGGAC
  GTATCTATTCATGGTGCCGTTGGTGCTTCAGAACTTGCAAATACCGCCAAAGCAGATGTCCCATACATCC
  TGAAGATGGAGTCTCACCCACACACCACCTGCTGGCCTGGGAGAACCCTCTACTTGCTAGCACCCAGCTT
  CCCCGACAAGCAGCGCTGGGTCACCGCCTTAGAATCTGTCGTCGCAGGTGGGAGAGTTTCTAGGGAAAAG
  GCCGAAGCCGATGCTGCACGTGACCATACTTCGAGTGAGCATCAGCCAGTGTGGGTTGAGAAATTACTTG
  GAAACTCTCTGCTGAAACTGGAGGGCGATGACCGGCTTGACATGAACTGCACCCTGCCCTTCAGTGACCA
  GGTGGTGCTGGTGGGCACGGAGGAAGGCCTCTACGCCCTGAATGTCTTGAAAAACTCCTTAACCCACATC
  CCAGGGATTGGCGCAGTCTTCCAAATTTACATCATCAAGGACCTGGAGAAGCTGCTCATGATAGCAGGGG
  AAGAGCGGGCTCTGTGCCTGGTGGACGTGAAGAAGGTGAAGCAGTCCCTGGCGCAGTCACACCTGCCTGC
  CCAGCCCGACGTCTCCCCCAACATATTCGAAGCCGTCAAAGGCTGCCACTTGTTCGCTGCTGGCAAGATC
  GAGAACAGCCTGTGCATCTGCGCCGCTATGCCAAGCAAAGTCGTCATCCTCCGCTACAATGACAACCTCA
  GCAAGTACTGCATCCGCAAGGAGATCGAGACCTCAGAGCCCTGCAGCTGTATCCACTTCACCAACTACAG
  CATCCTCATTGGGACCAACAAATTCTATGAGATCGACATGAAGCAGTACACGCTTGATGAGTTCCTGGAC
  AAGAACGACCATTCCTTGGCACCTGCTGTGTTCGCCTCCTCGTCCAACAGCTTCCCTGTCTCCATTGTGC
  AGGCGAACAGCGCCGGGCAGCGAGAAGAATACCTGCTGTGCTTCCACGAATTTGGGGTGTTCGTGGATTC
  TTACGGAAGACGTAGCCGCACAGATGATCTTAAGTGGAGTCGCTTACCTCTGGCCTTCGCCTACAGAGAA
  CCTTATCTGTTTGTGACTCACTTCAACTCCCTGGAAGTCATTGAGATCCAGGCCAGATCCTCACTGGGGA
  GCCCTGCCCGAGCATATCTGGAAATTCCAAACCCTCGCTACCTGGGCCCCGCGATTTCCTCCGGAGCGAT
  TTACCTGGCCTCCTCATACCAGGACAAGTTAAGGGTCATATGCTGCAAAGGAAACCTCGTGAAGGAGTCA
  GGCACTGAGCAGCACCGGGTGCCCTCCACCTCCCGCAGCCCCAACAAGCGAGGCCCACCAACATACAACG
  AGCACATCACCAAACGCGTGGCCTCCAGCCCGGCGCCACCGGAAGGCCCCAGCCACCCCCGAGAGCCAAG
  CACACCGCACCGCTACCGAGACAGAGAGGGTCGGACAGAGCTGCGCAGGGACAAGTCTCCAGGCCGCCCT
  CTGGAGCGGGAGAAGTCCCCAGGCCGAATGCTCAGCACTAGGAGAGAGCGGTCCCCAGGGAGACTGTTTG
  AAGACAGCAGCAGGGGCCGGCTGCCTGCAGGAGCAGTGAGGACCCCACTGTCCCAGGTTAACAAGGTCTG
  GGACCAGTCTTCAGTATAA
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCTGAAGAGGATCTGGCAGCAAATGATA
  TCCTGGATTACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-RsrII
• ACCN: BC023775
• Insert Size: 3099 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC023775, AAH23775
• RefSeq Size: 3506 bp
• RefSeq ORF: 3098 bp
• Locus ID: 12704
• Cytogenetics: 5 F
• Gene Summary: Plays a role in cytokinesis. Required for KIF14 localization to the central spindle and midbody. Probable RHO/RAC effector that binds to the GTP-bound forms of RHO and RAC1. It probably binds p21 with a tighter specificity in vivo. Displays serine/threonine protein kinase activity. Plays an important role in the regulation of cytokinesis and the development of the central nervous system. Phosphorylates MYL9/MLC2.[UniProtKB/Swiss-Prot Function]