Pik3cd (NM_001164052) Mouse Untagged Clone

CAT#: MC223024

Pik3cd (untagged) - Mouse phosphatidylinositol 3-kinase catalytic delta polypeptide (Pik3cd), transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Pik3cd
• Synonyms: 2410099E07Rik; 2610208K16Rik; AW545373; p110delta
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC223024 representing NM_001164052
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCCCCTGGGGTGGACTGCCCCATGGAGTTCTGGACCAAAGAGGAGAGCCAGAGCGTGGTTGTTGACT
  TCTTGCTGCCCACAGGGGTCTACTTGAACTTCCCCGTGTCCCGCAATGCCAACCTCAGCACCATCAAGCA
  GGTGCTGTGGCACCGTGCACAGTATGAGCCACTCTTCCACATGCTCAGTGACCCCGAGGCCTATGTGTTC
  ACCTGTGTGAACCAGACGGCGGAGCAGCAGGAGTTGGAGGATGAGCAGCGGAGGCTGTGCGACATCCAGC
  CCTTCCTGCCCGTGCTGCGCCTCGTGGCCCGAGAGGGGGACCGCGTGAAGAAGCTCATTAACTCCCAGAT
  CAGCCTCCTCATTGGCAAAGGTCTCCATGAGTTTGATTCCCTGCGGGACCCGGAAGTAAACGACTTCCGC
  ACTAAGATGCGCCAGTTTTGTGAAGAGGCTGCTGCTCACCGCCAGCAGCTGGGCTGGGTGGAATGGCTGC
  AGTACAGCTTCCCCCTGCAGCTGGAGCCCTCAGCAAGGGGTTGGCGGGCCGGCTTATTGCGTGTCAGCAA
  CCGAGCCCTGCTGGTCAACGTGAAGTTCGAGGGCAGTGAGGAGAGCTTCACCTTCCAGGTATCCACCAAG
  GACATGCCCCTGGCACTGATGGCCTGTGCCCTCCGAAAAAAGGCCACAGTGTTCCGGCAGCCTCTGGTGG
  AGCAGCCTGAGGAATATGCCCTGCAGGTGAACGGGAGGCACGAATACCTCTACGGCAACTACCCGCTCTG
  CCACTTTCAGTACATCTGCAGCTGCCTACACAGCGGGCTGACCCCTCATCTGACCATGGTCCACTCCTCC
  TCCATCCTTGCTATGCGGGATGAGCAGAGCAATCCTGCCCCCCAAGTACAGAAACCACGTGCCAAACCTC
  CCCCGATCCCTGCCAAGAAGCCCTCCTCTGTGTCCCTGTGGTCCCTGGAACAGCCATTCTCCATTGAGCT
  GATCGAGGGCCGAAAAGTGAATGCTGACGAGCGGATGAAGCTGGTTGTTCAGGCCGGGCTCTTCCATGGC
  AATGAGATGCTGTGCAAGACTGTGTCAAGCTCGGAGGTGAATGTATGCTCAGAGCCCGTGTGGAAGCAGC
  GACTGGAGTTCGATATCAGCGTCTGTGACCTCCCGCGCATGGCTCGACTCTGTTTTGCTCTCTATGCCGT
  CGTGGAGAAGGCTAAGAAGGCACGCTCCACAAAGAAGAAGTCTAAGAAGGCGGACTGCCCCATCGCTTGG
  GCCAACCTCATGCTATTCGACTACAAAGATCAGCTCAAGACGGGGGAGCGCTGCCTCTACATGTGGCCCT
  CTGTCCCAGATGAGAAGGGAGAGCTGCTGAATCCTGCGGGTACAGTGCGCGGGAACCCCAACACGGAGAG
  TGCCGCTGCCCTGGTCATCTACCTGCCTGAGGTGGCCCCCCACCCTGTGTACTTCCCCGCTCTGGAGAAG
  ATCCTGGAGCTGGGGCGTCACGGGGAGCGTGGGCGCATCACGGAGGAGGAGCTGCAGCTGCGGGAGATCC
  TGGAACGGCGGGGATCCGGGGAACTGTACGAACATGAGAAGGACCTGGTGTGGAAGATGCGCCACGAAGT
  CCAGGAGCATTTCCCAGAGGCGCTGGCCCGCCTGCTGCTGGTCACCAAGTGGAATAAACACGAGGATGTG
  GCCCAGATGCTCTATTTGCTGTGCTCCTGGCCCGAGCTGCCTGTGCTGAGCGCCCTGGAACTTCTGGACT
  TTAGCTTTCCCGACTGCTACGTGGGCTCCTTCGCCATCAAGTCCCTTCGGAAGCTGACGGACGATGAGCT
  CTTCCAGTACCTTCTGCAGCTGGTGCAAGTGCTCAAATATGAGTCCTACCTGGACTGCGAGCTGACCAAA
  TTCTTGCTGGGCCGAGCCCTGGCTAACCGCAAGATCGGACACTTCCTGTTCTGGCACCTCCGCTCTGAGA
  TGCACGTACCATCAGTGGCTCTGCGGTTTGGTCTCATCATGGAAGCCTACTGCAGAGGCAGCACCCACCA
  CATGAAGGTGCTGATGAAGCAGGGGGAAGCACTGAGCAAGCTTAAGGCACTGAATGACTTTGTGAAGGTG
  AGTTCCCAGAAGACCACCAAGCCCCAAACCAAGGAGATGATGCATATGTGCATGCGCCAGGAGACCTACA
  TGGAGGCCCTGTCCCACCTGCAGTCTCCACTCGACCCCAGCACCCTGCTGGAGGAAGTCTGTGTGGAGCA
  GTGCACCTTCATGGACTCCAAAATGAAGCCCCTGTGGATCATGTACAGCAGCGAGGAGGCGGGCAGTGCT
  GGCAACGTGGGCATCATCTTTAAGAACGGGGATGACCTCCGCCAGGACATGCTGACTCTGCAGATGATCC
  AGCTCATGGACGTCCTGTGGAAGCAGGAGGGCCTGGACCTGAGGATGACGCCCTACGGCTGCCTCCCCAC
  CGGGGACCGCACAGGTCTCATCGAGGTGGTCCTCCACTCGGACACCATCGCCAACATCCAGCTGAACAAA
  AGCAACATGGCGGCCACAGCTGCCTTCAACAAGGACGCCCTGCTCAACTGGCTCAAGTCCAAGAACCCTG
  GGGAGGCCCTGGATCGGGCCATTGAGGAATTCACCCTCTCCTGTGCTGGCTACTGTGTGGCCACATATGT
  TCTGGGCATCGGTGACCGGCACAGCGACAACATCATGATCAGAGAGAGTGGGCAGCTCTTCCACATTGAT
  TTTGGCCACTTTCTGGGGAACTTCAAGACCAAGTTTGGAATCAACCGAGAGCGCGTCCCCTTCATTCTCA
  CCTACGACTTTGTCCACGTGATCCAGCAGGGGAAGACTAACAACAGTGAGAAGTTTGAAAGGTTCCGCGG
  CTACTGTGAACGAGCCTATACCATCCTGCGGCGCCACGGGCTGCTTTTCCTCCATCTCTTCGCCCTGATG
  CGGGCCGCAGGTCTGCCTGAGCTTAGCTGCTCCAAAGATATCCAGTATCTCAAGGACTCTCTGGCACTGG
  GGAAGACGGAGGAAGAGGCGCTAAAGCACTTCCGGGTGAAGTTCAACGAAGCTCTCCGAGAAAGCTGGAA
  AACCAAAGTCAACTGGCTGGCGCACAATGTGTCCAAGGATAACCGACAGTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001164052
• Insert Size: 3132 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001164052.1, NP_001157524.1
• RefSeq Size: 4636 bp
• RefSeq ORF: 3132 bp
• Locus ID: 18707
• UniProt ID: O35904
• Cytogenetics: 4 E2
• Gene Summary: Phosphoinositide-3-kinase (PI3K) that phosphorylates PtdIns(4,5)P2 (Phosphatidylinositol 4,5-bisphosphate) to generate phosphatidylinositol 3,4,5-trisphosphate (PIP3). PIP3 plays a key role by recruiting PH domain-containing proteins to the membrane, including AKT1 and PDPK1, activating signaling cascades involved in cell growth, survival, proliferation, motility and morphology. Mediates immune responses. Plays a role in B-cell development, proliferation, migration, and function. Required for B-cell receptor (BCR) signaling. Mediates B-cell proliferation response to anti-IgM, anti-CD40 and IL4 stimulation. Promotes cytokine production in response to TLR4 and TLR9. Required for antibody class switch mediated by TLR9. Involved in the antigen presentation function of B-cells. Involved in B-cell chemotaxis in response to CXCL13 and sphingosine 1-phosphate (S1P). Required for proliferation, signaling and cytokine production of naive, effector and memory T-cells. Required for T-cell receptor (TCR) signaling. Mediates TCR signaling events at the immune synapse. Activation by TCR leads to antigen-dependent memory T-cell migration and retention to antigenic tissues. Together with PIK3CG participates in T-cell development. Contributes to T-helper cell expansion and differentiation. Required for T-cell migration mediated by homing receptors SELL/CD62L, CCR7 and S1PR1 and antigen dependent recruitment of T-cells. Together with PIK3CG is involved in natural killer (NK) cell development and migration towards the sites of inflammation. Participates in NK cell receptor activation. Have a role in NK cell maturation and cytokine production. Together with PIK3CG is involved in neutrophil chemotaxis and extravasation. Together with PIK3CG participates in neutrophil respiratory burst. Have important roles in mast-cell development and mast cell mediated allergic response. Involved in stem cell factor (SCF)-mediated proliferation, adhesion and migration. Required for allergen-IgE-induced degranulation and cytokine release. The lipid kinase activity is required for its biological function.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) uses an alternate in-frame splice site in the central coding region, compared to variant 4. The promoter and 5' UTR for this variant has not been determined. The resulting isoform (a) lacks a 1-aa internal segment, compared to isoform a. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.