Mthfd1l (NM_172308) Mouse Untagged Clone

CAT#: MC222931

Mthfd1l (untagged) - Mouse methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1-like (Mthfd1l), nuclear gene encoding mitochondrial protein, transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Mthfd1l
• Synonyms: 2410004L15Rik; AI647056; Fthfsdc1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC222931 representing NM_172308
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCGTCCGCCTCCCTCTCCTGCTTCGCCAGCTCGGCCGCCAGCAGCTGCCCTCGGGCCCGGCGTGTC
  GCCTCCGTGAGCTGTGTCGCTCAGGCAGCAGAAGCAGCAGCAGCGGAGGTGGTGACCCCGAGGGGTTACG
  CGGGCGGCGGCTGCAGGATGGCCAAACCTTCAGCAGCCATGGCCCAGGCAATCCGGAAGCCCCCGGGATG
  GACTCCATCGTCAGAGACGTCATTCACAATTCCAAGGAAGTGCTGAGCTTGTTGCAAGAAAAGAACCCTG
  CCTTTAAGCCAGTGCTTGTGGTTATTCAGGCCGGTGATGACAATTTGATGAAGGATATGAACCAGAATTT
  GGCCAAGGAGGCTGGTCTGGACATCACTCACATCTGCTTGCCTCCAGACAGCGGAGAGGATGAGATCATA
  GATGAAATCCTGAAGATTAATGAAGACCCCAGAGTGCATGGCCTTACCCTTCAGATCTCTGAAGATTCAT
  TGAGCAACAAAGTTCTCAATGCTTTGAAACCAGAAAAAGATGTGGACGGGGTGACCGACATCAATCTGGG
  GAAGCTCGTACGAGGTGACGCTCCGGAGTGTTTTGTTTCACCCTTGGCCAAAGCTGCTGTAGAACTTGTT
  GAAAAGTCAGGGATCACTTTGGATGGAAAGAAGGTCTTGGTCGTAGGGGCGCACGGGCCCCTGGAAGCTG
  CCCTGCAGTGGCTTTTCCAGAGAAAAGGATCTATGACAATGAGCTGCCCCTGGGCAACACCTCAGCTTCC
  AGACAAGCTTCGAGAGGCAGACATTGTGGTCTTAGGCTCACCTAAGCCAGAAGAGATTCCTGCTGTGTGG
  ATTCCATCAGGAACCACCATTCTCAATTGCTTCCATGACTTCTTGTCAGGGAAGCTGAGCGGAGGTTCTC
  CTGGGGTCCCCGTGGACAAACTCATTGCAGAAGAGAGCGTGAGTCTCCTTGCTGCGGCTCTGCGCATTCA
  GAACATGGTGAGCAGCGGCAGGAGGTGGCTCAGAGAGCAGCAACATCGAAGATGGCGACTGCACTGCTTG
  AAACTGCAGCCTCTCTCACCTGTGCCCAGTGATATTGAGATTTCTCGAGGACAGACTCCAAAAGCTGTGG
  ATGTCCTTGCCAAGGAGATAGGATTGCTTGCAGATGAAATTGAAATCTATGGCAAGAGCAAAGCCAAAGT
  CCAATTGTCCCTGCTGGAGAGGTTAAAGGATCAAACAGATGGAAAATACGTCTTGGTTGCTGGGATCACG
  CCCACCCCGCTCGGAGAAGGGAAGAGTACAGTCACCATCGGACTAGTGCAGGCGCTGACCGCTCACCTGA
  AAGTCAACTCCTTCGCCTGTCTGAGGCAGCCTTCTCAGGGACCCACTTTTGGAGTGAAAGGAGGAGCTGC
  AGGAGGTGGATATGCTCAGGTCATCCCCATGGAGGAGTTCAACCTTCACCTGACTGGGGACATCCATGCC
  ATCACTGCCGCTAATAACTTGCTGGCCGCAGCCATCGACACGAGAATTTTACATGAGAGCACTCAAACAG
  ACAAGGCTCTCTACAATCGACTGGTTCCCTTAGTGAATGGTGTCAGAGAATTTTCAGAAATTCAACTTTC
  TCGGCTGAAGAAACTGGGGATACATAAGACTGATCCAAGCACACTGACCGAGGAGGAAGTGAGGAAGTTC
  GCCCGCCTCAACATTGACCCCGCAACCATCACGTGGCAGAGAGTATTGGACACGAACGACCGATTTCTGA
  GAAAAATAACCATTGGACAGGGAAGTACAGAGAAAGGATATTCCCGGCAGGCACAGTTTGACATTGCAGT
  GGCCAGCGAGATCATGGCCGTGCTGGCCTTGACTGATAGCCTCACAGACATGAAAGAGCGGCTAGGAAGA
  ATGGTGGTGGCCAGTGACAAAGACGGGCAGCCTGTGACAGCTGAGGACTTGGGGGTGACCGGCGCTCTGA
  CAGTTTTGATGAAAGACGCCATCAAGCCAAATCTGATGCAGACCCTTGAAGGAACACCTGTGTTTGTGCA
  CGCTGGTCCCTTTGCCAACATCGCTCACGGCAACTCATCAGTATTAGCTGATAAAATCGCGCTGAAATTG
  GTCGGCGAAGAAGGGTTTGTGGTGACTGAAGCTGGCTTTGGGGCTGATATTGGGATGGAGAAATTCTTCA
  ATATCAAATGCCGAGCTTCTGGCCTGGTGCCCAACGTTGTCGTGCTGGTAGCCACCGTTCGAGCTCTGAA
  GATGCATGGAGGCGGGCCGAGTGTAACTGCTGGGGTTCCTCTCAAGAAAGAATATACAGAGGAGAACATT
  CAGCTGGTAGCTGATGGCTGCTGCAACCTACAGAAGCAAATTCAGATCGCTCAGCTCTTCGGGGTGCCGG
  TGGTGGTGGCACTGAATGTCTTCAAGACGGACACCCGTGCAGAGATTGACTTGGTGTGTGAGCTCGCTAA
  ACGGGCCGGTGCCTTTGATGCGGTCCCCTGCTATCACTGGTCGGCTGGAGGGAAAGGATCTGTGGACTTG
  GCCCGGGCTGTGAGAGAAGCTGCGAATAAAAGAAGCCGCTTCCAGTTCCTGTATGATGTTCAGCTTCCCA
  TCGTGGAAAAGATAAGAGTCATTGCCCAGACTGTCTATGGAGCCAAGGACATTGAGCTGTCTCCTGAGGC
  GCAGTCCAAAATCGATCGCTATACGCAACAGGGTTTTGGAAATTTGCCCATTTGCATGGCGAAGACCCAC
  CTCTCTCTCTCTCATGAACCTGACAAAAAGGGAGTGCCGAGGGATTTCACTCTGCCCATCAGTGATGTCC
  GGGCCAGCATCGGGGCTGGGTTCATTTATCCTTTGGTGGGAACGATGAGCACAATGCCAGGACTGCCCAC
  CCGACCCTGCTTTTATGATATAGACCTTGACACAGAAACGGAGCAAGTTAAAGGCTTGTTCTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_172308
• Insert Size: 2934 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_172308.4, NP_758512.3
• RefSeq Size: 3731 bp
• RefSeq ORF: 2934 bp
• Locus ID: 270685
• UniProt ID: Q3V3R1
• Cytogenetics: 10 A1
• Gene Summary: May provide the missing metabolic reaction required to link the mitochondria and the cytoplasm in the mammalian model of one-carbon folate metabolism in embryonic an transformed cells complementing thus the enzymatic activities of MTHFD2.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) differs in the 3' UTR compared to variant 1. Variants 1, 2 and 3 encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.