Mical2 (NM_177282) Mouse Untagged Clone

CAT#: MC222856

Mical2 (untagged) - Mouse microtubule associated monoxygenase, calponin and LIM domain containing 2 (Mical2), transcript variant B, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Mical2
• Synonyms: 5330438E18Rik; 9530064J02; MICAL-2; mKIAA0750
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC222856 representing NM_177282
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGAGAGAATGAAGATGAGAAGCAGGCGCAGGCCAGCCAGGTCTTCGAGAACTTTGTGCAAGCTACCA
  CATGCAAAGGGACCCTCCAGGCCTTCAACATCCTCACCTGCCTCCTGGACCTAGATCCGCTGGACCATAG
  GAACTTCTACTCCCAGCTCAAGTCCAAGGTGAACACCTGGAAGGCCAAAGCCCTGTGGCACAAACTGGAT
  AAGCGCGGCTCCCACAAGGAGTACAAGCGAGGAAAAGCCTGCTCGAACACTAAGTGTCTCATCGTCGGAG
  GAGGACCATGTGGCTTGCGCACTGCCATTGAACTTGCCTACCTGGGAGCCAAAGTGGTTGTGGTGGAGAA
  GAGGGACACCTTCTCCCGGAACAATGTCCTGCACCTCTGGCCCTTCACTATCCATGACCTGCGGGGCCTG
  GGGGCCAAGAAGTTCTATGGGAAATTCTGTGCTGGCTCCATCGACCACATCAGTATCCGACAACTGCAGC
  TTATCCTCTTCAAGGTGGCCCTGATGCTGGGAGTGGAGGTCCACGTGAATGTGGAGTTTGTGAGGGTGCT
  GGAGCCTCCTGAAGACCAAGAGAATCAAAAAGTTGGATGGCGGGCAGAATTCCTTCCTGCAGACCACGCC
  CTGTCTGACTTTGAGTTTGATGTCATCATCGGTGCTGACGGTCACAGGAACACGCTAGAAGGCTTCAGGA
  GGAAAGAGTTCCGAGGGAAGCTGGCCATCGCCATCACCGCCAACTTCATAAACAGGAACAGCACAGCTGA
  GGCCAAGGTGGAGGAGATCAGTGGTGTTGCCTTCATCTTCAACCAGAAGTTCTTCCAGGACCTGAAGGAA
  GAAACAGGGATTGATCTCGAGAACATTGTTTACTATAAGGACAGTACCCACTACTTTGTCATGACAGCCA
  AGAAGCAGAGCCTGCTGGACAAGGGCGTCATCCTTAATGACTACATTGACACAGAGATGCTGCTGTGTTC
  GGAGAATGTGAACCAGGACAACCTGCTCTCCTACGCCAGAGAAGCCGCTGACTTTGCCACCAACTACCAG
  CTGCCATCCTTAGACTTTGCCATCAATCACAACGGGCAGCCTGACGTGGCCATGTTCGACTTCACCTCCA
  TGTATGCCTCAGAGAACGCAGCTCTGATGCGTGAGCGCCAGGCACACCAGCTGCTCGTGGCTCTTGTGGG
  CGACAGCCTGCTTGAGCCATTTTGGCCCATGGGCACAGGCTGTGCCCGAGGCTTCCTGGCAGCCTTTGAC
  ACGGCATGGATGGTGAAGAGCTGGGACCAGGGCACCCCTCCCCTGGAGGTATTAGCTGAAAGAGAGAGTC
  TTTACAGGCTGTTACCTCAGACAACCCCAGAGAACATCAACAAAAATTTTGAGCAGTACACATTGGACCC
  AGCCACGCGGTACCCAAACCTCAACCTGCACTGCGTCAGGCCTCACCAGGTGAAGCATTTGTACATCACT
  AAGGAGATGGACCGCTTCCCTCTCGAGAGATGGGGCTCAGTGAGGAGATCTGTCAGCCTCTCCAGGCGGG
  AGTCAGACATCCGGCCTAACAAGCTTTTAACCTGGTGCCAGCAGCAGACCAAGGGTTACCAGCACGTCAG
  AGTCACTGACCTGACCACATCCTGGCGCAGCGGCTTGGCCCTGTGTGCCATCATCCACAGCTTCCGGCCA
  GAGCTGATCAACTTTGACTCGCTGAATGAAGATGACGCTGTGGAGAACAACCAACTGGCATTTGATGTGG
  CCAAGCGTGAGTTTGGGATCCTGCCTGTGACCACAGGCAAAGAGATGGCATCTACCCAGGAGCCAGACAA
  GCTCAGCATGGTCATGTACCTCTCCAAGTTCTATGAGCTCTTCCGGGGCACTCCACTGAGACCCATGGAT
  TCCTGGCGTAAAAACTATGGAGAAAATGCTGACTTTGGCTTGGGCAAAACATTCATTCAGAATAACTATC
  TCAACCTCACATTGCCCAGAAAGAGGACCCCACGGGTAGACACCCAGACTGAAGAGAATGACATGAACAA
  GAGGCGGCGACAAGGCTTCAACCACCTGGAAGAGCTGCCATCCTTCTCCAGCCGCAGCCTGGGCTCCAGT
  CAAGAGTATGCTAAAGAAAGTGGCAGTCAGAACAAGGTCAAGCACATGGCCAATCAGCTGCTAGCCAAGT
  TTGAGGAGAACACTCGGAACCCTTCAGTCGTGAAACAGGAATCCCCGAGAAAGGCATTCCCCCTGAGCCT
  GGGCGGCAGAGACACCTGCTACTTTTGTAAGAAGCGTGTATACATGATAGAGCGGCTGAGTGCTGAGGGC
  CACTTTTTCCACCAAGAGTGCTTCCGTTGCAGCGTCTGCAGTGCCACCCTGCGCCTGGCTGCCTATGCCT
  TTGACTGCGATGAAGGCAAATTTTACTGCAAGCCCCATTTTGTTCACTGCAAAACCAGTAGCAAACAGCG
  AAAGAGACGGGCAGAGCTGAATCAGCAAAGAGAGGAGGAAGGAACATGGCAGGAGCAGGAAGCACCTCGG
  AGGGATGTACCCACAGAAAGCTCTTGTGCAGTGGCGGCCATCAGCACGCCAGAAGGCAGCCCCCCAGGTA
  CCTCCACCTCCTTCTTTAGGAAGGCACTCAGCTGGCCTCTCCGGCTGACAAGAGGCCTGCTCAACCTGCC
  CCAGAGCCTGCTTAGGTGGATGCAGGGACTCCAGGAGGCTGCTGGCCACCATGTGCGGGACAATGCTCAC
  AACTACTGCTTCATGTTTGAGCTCCTGAGCCTGGGGCTGCTACTGCTCTGGGCTTTCTCCAAGGTCCTGG
  CTGCCATGTACAGAGAGTCTGAGGAGTCTCTGGAGAACATCCGCAGCTGGCTGCTCAGGTTTATCCCAGT
  GAAGCTGCAGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_177282
• Insert Size: 2883 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_177282.5, NP_796256.1
• RefSeq Size: 6689 bp
• RefSeq ORF: 2883 bp
• Locus ID: 320878
• UniProt ID: Q8BML1
• Cytogenetics: 7 F1
• Gene Summary: Nuclear monooxygenase that promotes depolymerization of F-actin by mediating oxidation of specific methionine residues on actin to form methionine-sulfoxide, resulting in actin filament disassembly and preventing repolymerization (PubMed:23911929, PubMed:23927065). In the absence of actin, it also functions as a NADPH oxidase producing H(2)O(2) (By similarity). Acts as a key regulator of the SRF signaling pathway elicited by nerve growth factor and serum: mediates oxidation and subsequent depolymerization of nuclear actin, leading to increase MKL1/MRTF-A presence in the nucleus and promote SRF:MKL1/MRTF-A-dependent gene transcription. Does not activate SRF:MKL1/MRTF-A through RhoA (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (B) lacks 5 alternate exons, resulting in the loss of an in-frame segment in the 3' coding region, and uses an alternate splice site in the 3' coding region, compared to variant 1. The resulting protein (isoform B) is shorter and has a distinct C-terminus, compared to isoform A. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.