Map3k13 (NM_172821) Mouse Untagged Clone

CAT#: MC222837

Map3k13 (untagged) - Mouse mitogen-activated protein kinase kinase kinase 13 (Map3k13), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Map3k13
• Synonyms: C130026N12Rik; LZK
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC222837 representing NM_172821
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCAACCCTCAGGAGCACCTGAGCTGCTCCTCTTTGCCACACTTGCCCTTGACTGAGAACAAAACCT
  CTGGTGGACGAAATGAGCTTGCAGCTATGGGGAACCACCCTTCACCCAAGCTCCCTGAGGACCCTCAGGA
  GAGGGGGGCTATACAGTCAGAGCTGATGGAGATCACAGGCAGCCCGATCTCCACAACAGTGTTGACCAGT
  GTAAGCGAGGATTCTAGGGGCCAGTTTGAGAACAGTGTTCTTCAGCTAAGGGAACAGGATGAGTCAGAGA
  TGACCCTGTCTCTGGGGAACAGCAACACTGTGGATGGAGAGAACACGAACGGACCAGAAGACATCAAGAT
  TCAGTTCAGCAGGTCAGGCAGCGGCAGCGGCGGGTTTCTCGAAGGACTGTTTGGATGTCTCAGGCCCGTG
  TGGAATATCATTGGGAAGGCATATTCCACTGATTACAAGCTGCAGCAGCAAGATACTTGGGAAGTGCCAT
  TTGAGGAAATCTCAGAGCTGCAATGGCTGGGTAGTGGAGCCCAAGGAGCTGTCTTCCTGGGCAAATTCCG
  AGCAGAGGAGGTGGCCATCAAGAAGGTGAGAGAACAAAATGAGACGGACATCAAGCATCTGAGGAAGCTG
  AAGCACCCTAACATCATCGCCTTCAAGGGTGTTTGCACTCAGGCCCCGTGCTACTGCATCATCATGGAGT
  ACTGTGCCCACGGACAGCTGTACGAGGTCCTGAGAGCTGGCCGGAAGATCACGCCCCGGCTGCTTGTGGA
  CTGGTCCACGGGAATCGCCAGTGGAATGAATTATCTCCATCTCCACAAGATTATACACCGTGACCTCAAG
  TCGCCCAATGTTTTAGTGACTCACACAGATGCAGTGAAAATTTCAGACTTTGGCACATCTAAGGAACTCA
  GTGATAAAAGCACCAAGATGTCCTTCGCTGGCACCGTCGCGTGGATGGCACCAGAGGTGATCCGGAATGA
  GCCTGTCTCGGAGAAGGTGGACATATGGTCATTTGGTGTGGTACTTTGGGAGCTGCTGACAGGGGAGATT
  CCCTACAAGGATGTGGACTCTTCCGCCATCATTTGGGGTGTCGGCAGCAACAGCTTGCATCTCCCAGTTC
  CTTCCACTTGCCCGGATGGATTCAAAATCCTTATGAAACAGACATGGCAGAGTAAGCCTCGCAACCGACC
  TTCTTTCAGGCAGACGCTCATGCATCTGGATATCGCGTCTGCAGATGTGCTCGCCACCCCTCAAGAAACT
  TACTTCAAGTCTCAGGCTGAATGGAGAGAAGAAGTGAAGAAACATTTTGAGAAAATCAAAAGTGAAGGGA
  CCTGTATACACCGATTAGATGAAGAACTGATTCGCCGGCGCAGGGAGGAGCTCAGGCATGCTCTGGATAT
  TCGGGAGCACTACGAGAGAAAGCTCGAACGGGCTAACAATCTGTACATGGAGCTGAGCGCCATCATGCTG
  CAGCTGGAGATGCGCGAGAAGGAGCTCCTTAAGCGAGAGCAAGCAGTGGAAAAGAAGTACCCTGGAACCT
  ACAAACGACACCCTGTCCGTCCAATCATCCACCCGAATGCCATGGAGAAACTCATGAAAAGGAAGGGTGT
  GCCTCACAAGGCTGGGGTGCAGACTAAGCGGCCAGACCTGTTGCGATCCGAAGGCATTCCTAGTACAGAA
  GCGGTTCCCACTGCATCCCCCCTGTCTGGAAGTCCCAAAATGTCCACCGCAAGCAGCAGAAGCCGGTACC
  GAAGCAAACCACGTCACCGCCGAGGGAACAGTAGAGGCAGCCATAGCGACTTCGCAGCAATCTTGAAAAC
  CCAGCCCGCCCAGGAAAATTCACCCCACCCCACCTACATGCACCACACTCAGGCTCAGTGCGCTTCAGTC
  CATCAACATAACCCTCTCCAGCAGCAATACCAGCAAATTCCTCCTGCCCAGCCTCAGAGCCGGCATCCCA
  GACTCAATGCGCATGGGCAGGACATTGCAACCTGCGCCAATAACCTGAGGTACTTTGGCCCCGCAGCGGC
  CCTGCGGAGTCCACTCAGCAACCATGCACAAAGACAGATGCCTGGCTCAAGCCCCGACCTCATCTCCACA
  GCCATGGCTGCAGATTGGAGAAATTCTGAACTTGATCAAGACCAAGTTGGACCCTGGGGCTGTTGTCAGG
  CTGAACCCTATGATCCCTGTTTCCAGTGCAGGCCAGAACATTCTGGGTCTTTAGATGTTCCCACTACTGA
  GCCAGTGGGGAGGAGCCCAGACCTTTCCAGTTCACCAGCACACAATCCTCTTTCAGGAAATGCCCAAGGC
  TCTGAGAGAACAGGGGCAAATGGATTCAGTGGCTGTCAGTCTGGTATTTCTCATCAATTCACACCCCCAA
  TGCTACCTCAGAAGACACGGCCCCTTCAGAAGAGTGGCGATGACTCCTCAGAAGAGGAAGGGGAAGTGGA
  CAGTGAAGTAGAATTTCCTCGACGACAGAGGCCTCACCGCTGCATTAGCAGTTACCAGTCATACTCAACC
  TTTAGCTCCGAGAACTTCTCTGTGTCTGATGGAGAGGAGGGCAACACTAGTGACCACTCGAACAGTCCCG
  ATGAATCCGCCAACAGACGGCAGGACCGCCTGGCAGAGACGCTGGACGACCTGCTGTCTCAGACACCCGA
  GGCCCCCATCGAGATCTCCTCCCACTCAGATGGGCTGTCTGACAAGGAGTGTGCTGTTCGGCGCGTGAAG
  ACACAGATGTCCCTAGGCAAGCTGTGTGCCGAGGAGCGCGGCTACGAGAACCCTGTGCAGTTTGGAGACT
  CGGACTGTGACTCTTCAGAGGGAGAGTGTTCCGATGCCACAGTTAGGACCAGCAAGAACTACAGCTCTGC
  CACCTGGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_172821
• Insert Size: 2880 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_172821.3, NP_766409.2
• RefSeq Size: 6709 bp
• RefSeq ORF: 2880 bp
• Locus ID: 71751
• UniProt ID: Q1HKZ5
• Cytogenetics: 16 B1
• Gene Summary: Activates the JUN N-terminal pathway through activation of the MAP kinase kinase MAP2K7. Acts synergistically with PRDX3 to regulate the activation of NF-kappa-B in the cytosol. This activation is kinase-dependent and involves activating the IKK complex, the IKBKB-containing complex that phosphorylates inhibitors of NF-kappa-B (By similarity).[UniProtKB/Swiss-Prot Function]