Smcr8 (NM_001085440) Mouse Untagged Clone

CAT#: MC222720

Smcr8 (untagged) - Mouse Smith-Magenis syndrome chromosome region, candidate 8 homolog (human) (Smcr8), transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Smcr8
• Synonyms: 2310076G09Rik; AI642055; D030073L15Rik
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC222720 representing NM_001085440
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATCAGCGCCCCTGATGTGGTGGCCTTCACCAAGGAAGATGAATACGAGGAAGAACCTTACAATGAGC
  CCGCTTTGCCTGAGGAGTACTCAGTCCCTCTCTTTCCTTATGCCAGCCAGGGGGCAAACCCCTGGTCTAA
  ACTGTCTGGGGCCAAGTTCTCCAGGGACTTCATCCTCATTTCCGAGTTCTCTGAGCAGGTGGGACCCCAG
  CCCTTGCTTACCATCCCCAATGACACCAAAGTTTTTGGCACTTTTGATCTTAATTACTTCTCTTTGCGGA
  TTATGTCGGTGGATTACCAGGCTTCATTCGTAGGCCATCCTCCCGGTTCTGCCTACCCCAAGTTGAACTT
  TGTGGAAGACTCTAAAGTGGTACTGGGAGACTCTAAGGAAGGGGCCTTTGCATATGTGCACCACCTCACC
  TTGTACGACCTGGAGGCCAGGGGCTTTGTGAGGCCCTTTTGTATGGCTTATATCTCCGCAGACCAGCATA
  AAATCATGCAGCAGTTCCAAGAGCTCTCGGCCGAATTTTCCAAAGCATCTGAGTGCTTGAAGATGGGCAA
  CAGGAAGGCATTCGCTGGGGAACTTGAAAAAAAGCTGAAAGACTTGGATTACACAAGGACAGTGCTACAC
  ACAGAAACCGAGATCCAGAAGAAAGCCAACGACAAGGGTTTTTATTCTTCTCAGGCGATTGAGAAAGCCA
  ATGAACTGGCCAATGTGGAGAAGTCCATCATCGAACATCAAGATTTGCTGAGGCAGATCCGCTCATACCC
  TCGTCAGAAGACGAAGATCCCTGACTTGCAGCCTGGTGATACAGAGCATACCCAGGATCAGGCTGACCAG
  GTATCCACTACCTCTAATCCTGAGGAGTCCGCTAATGCAGACCTTTATACCTGCAGACCGGCTTACACCC
  CCAAACTCATCAAAGCAAAGTCCACCAAGTGTTTTGACAAGAAGTTGAAGACCTTGGAGGAACTCTGTGA
  CACTGAGTATTTCACTCAGACCCTGGCCCAGCTTAGCCACATTGAACACATGTTCAGAGGAGACCTGTGC
  TACCTCCTGACCAGTCAGATTGACAGAGTGCTTCGAAAACAACAGCCCATAACGAATTTCCTCTTTGAAG
  ATTTTGTAGAGGTGGATGACAGGATGGAAAAGCAAGAGAATGTACCCTCTCAGCCCAGTCAGGACAGGCT
  GCCTCCCAAGCCTGTAGAAGAATGCCCCATTCCTAAAGTGTTAATTAGCGTTGGCTCTTACAAGTCCAGT
  GTGGAGTCTGTATTGATCAAGATGGAGCAGGAACTTGGCGATGAAGAGTACACGGGGGTGGAGGCAACAG
  AGGCACGCAGTTTTGACCCCCAAGAGAACCTGGACTACCTGGATATGGATATGAAAGGGAGCATCAGTAG
  TGGGGAAAGCATTGAGGTGCTGGGCACCGAGAAGTCAGCCTCTGTGTTGTCGAAATCTGACAGCCAGGCC
  AGCCTCACCGTGCCATTGAGCCCCCATGTAGTCCGTAGCAAAGCGGTCAGCCACAGGACAATCAGCGAGG
  ACAGCATTGAAGTCTTAAGTACCTGCCCTTCTGAGGCCCTCATTCCTGATGACTTTAAGGCCAGTTACCC
  AAGTGCCATTAATGAAGAAGAAGCCTATGCGGATAATGAGGGGGCCATCCATTTCCAGGCAAGTGCCGGC
  TCACCAGAACCGGATGAGACTCAGGAGGGCAACTTGGAAAATATCCCATCCCAAATAGACTCCAGCTGCT
  GTATTGGAAAGGAGAGTGAAGGTCACTTGGTGCCTCTCCCAACCCCAGCCTACACTCTTTCTGATGAGGA
  CAGTGTGGTGAGCATTCCCCCACAGCGCTACATACAGAAGGACCAGGGGCTCCACGTGGACTTCGGAGTG
  GAAAACACTGACCCTTCTCCCCGAGACAACAGTTGTGAAATGTTCCCAGCTTATGAGCTGGATCCAAGCT
  GCCTTCTGGCTAGCCGAGATGTTAGTAAGATGAGCCTGGATAACTACTCGGATACCACTAGCTACATGGG
  CAGTGCGGCCTCTACCAGCTCAGACAGAATCCCCTCAGCACCTCCTGCTGGCCTATCCTCTGAGAGGCAC
  AAAAAGAGAGCTGGCCAGAATGCCTTAAAATTCATCCGCCAGTACCCTTTTGCCCACCCAGCCATCTACT
  CCCTGCTCAGTGGGAGGACACTTGTGGTCCTGGGCGAAGATGAAACCATTGTCAGGAAGCTGGTGACTGC
  ACTGTCCATCTTTGTTCCCAACTATGGCTGCTATGCCAAGCCGGTGAAGCACTGGATTTCTTCCCCTTTG
  CATATTATGGATTTCCAGAAGTGGAAGCTTATTGGCCTACAAAGAGTGGCCTCTCCTGCCAATGTGGGTA
  CCCTCCATACCCTGAGCCGTTACAGCCGTTATACAAGCATCCTGGACCTGGACAGCAAGACCCTACGCTG
  TCCCCTATACAGGGGTACACTGGTACCCCGCCTGGCTGACCATCGCACTCAGATCAAGCGGGGCAGCACA
  TACTACCTTCACGTCCAGAGCATGCTCACCCAGCTCTGCTCCAAGGCATTCCTCTACACCTTTTGTCACC
  ACTTGCATCTGCCCGCCCACAGTGAGGAGACACAAGAAGCAGTGGCCAGCAGACAAACCAGCTTCCTAAA
  GCTAAACCTGGGGCTCGTGAACGAGGACATCAGAGTGGTACAGTACCTGGCTGAGCTGTTGAAACTGCAC
  TATATGCAGGAGTCACCGGGGACCACCCACCCACTGCTCAGGTTTGACTACGTCCCCAGCTTTTTGTACA
  AAATCTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001085440
• Insert Size: 2808 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001085440.1, NP_001078909.1
• RefSeq Size: 7394 bp
• RefSeq ORF: 2808 bp
• Locus ID: 237782
• UniProt ID: Q3UMB5
• Cytogenetics: 11 B2
• Gene Summary: Component of the C9orf72-SMCR8 complex, a complex that has guanine nucleotide exchange factor (GEF) activity and regulates autophagy (PubMed:27617292). In the complex, C9orf72 and SMCR8 probably constitute the catalytic subunits that promote the exchange of GDP to GTP, converting inactive GDP-bound RAB8A and RAB39B into their active GTP-bound form, thereby promoting autophagosome maturation (By similarity). The C9orf72-SMCR8 complex also acts as a negative regulator of autophagy initiation by interacting with the ATG1/ULK1 kinase complex and inhibiting its protein kinase activity (PubMed:27617292). Acts as a regulator of mTORC1 signaling by promoting phosphorylation of mTORC1 substrates (By similarity). In addition to its activity in the cytoplasm within the C9orf72-SMCR8 complex, SMCR8 also localizes in the nucleus, where it associates with chromatin and negatively regulates expression of suppresses ULK1 and WIPI2 genes (By similarity).[UniProtKB/Swiss-Prot Function]