Grm7 (NM_177328) Mouse Untagged Clone

CAT#: MC222604

Grm7 (untagged) - Mouse glutamate receptor, metabotropic 7 (Grm7), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Grm7
• Synonyms: 6330570A01Rik; BB176677; C030018L03; E130018M02Rik; Gpr1g; Gprc1g; mGluR7; SMN2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC222604 representing NM_177328
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTCCAGCTGGGGAAGCTGCTCCGCGTCCTGACTTTGATGAAGTTCCCCTGCTGCGTGCTGGAGGTGC
  TCCTGTGCGTGCTGGCGGCGGCGGCGCGCGGCCAGGAGATGTACGCCCCGCACTCGATCCGGATCGAGGG
  GGACGTCACCCTTGGGGGGCTGTTCCCAGTGCACGCCAAGGGTCCCAGCGGAGTGCCCTGCGGCGACATC
  AAGAGGGAGAATGGGATCCACAGGCTGGAAGCTATGCTTTATGCTCTCGACCAGATCAACAGCGATCCCA
  ACCTGCTGCCCAACGTAACGTTAGGCGCGCGGATCCTGGACACTTGTTCCAGGGACACTTATGCGCTCGA
  ACAGTCGCTCACTTTCGTCCAGGCGCTTATCCAGAAGGACACCTCCGACGTGCGTTGCACCAACGGAGAG
  CCCCCGGTTTTCGTCAAGCCAGAGAAAGTAGTTGGAGTGATTGGGGCTTCGGGGAGCTCCGTCTCCATCA
  TGGTAGCCAACATCTTGAGGCTTTTCCAGATACCTCAGATTAGTTATGCATCCACGGCTCCTGAACTCAG
  TGATGACCGTCGCTATGACTTCTTCTCTCGCGTGGTCCCACCTGATTCCTTCCAAGCCCAAGCTATGGTA
  GACATTGTAAAGGCATTGGGCTGGAATTATGTATCTACTCTTGCATCTGAAGGAAGCTATGGAGAGAAAG
  GTGTGGAGTCCTTCACACAAATTTCCAAAGAAGCAGGTGGACTCTGCATTGCCCAGTCCGTGAGAATTCC
  CCAAGAACGCAAAGACAGGACCATTGACTTTGATAGAATTATCAAACAGCTCTTGGACACCCCCAACTCC
  AGGGCTGTCGTGATTTTTGCCAACGATGAGGATATAAAGCAGATCCTTGCAGCAGCCAAAAGAGCTGACC
  AAGTAGGACATTTTCTCTGGGTTGGGTCAGACAGCTGGGGTTCCAAAATCAACCCACTGCATCAGCATGA
  AGATATTGCAGAAGGAGCCATCACCATCCAACCTAAGAGAGCGACTGTGGAAGGATTTGATGCTTACTTC
  ACATCCCGGACACTTGAAAACAACAGGAGAAATGTATGGTTTGCCGAATACTGGGAAGAAAACTTCAACT
  GCAAGTTGACAATTAGTGGATCCAAAAAAGAAGACACGGATCGCAAATGCACAGGACAGGAGCGAATTGG
  AAAAGACTCTAATTATGAGCAGGAGGGTAAAGTACAGTTTGTGATCGATGCTGTCTATGCCATGGCACAT
  GCCCTTCATCACATGAATAAGGATCTGTGTGCTGACTACCGGGGAGTGTGCCCAGAAATGGAGCAAGCAG
  GGGGCAAGAAGTTGTTGAAGTATATCCGCAATGTTAACTTCAATGGTAGTGCTGGAACCCCAGTGATGTT
  TAACAAAAATGGTGATGCTCCAGGACGATATGACATCTTCCAGTACCAGACAACAAACACTACCAATCCT
  GGTTATCGTCTCATTGGGCAGTGGACAGATGAACTGCAGCTCAATATAGAGGACATGCAGTGGGGCAAAG
  GAGTCCGGGAGATCCCACCCTCTGTGTGCACATTGCCATGCAAGCCTGGGCAGAGGAAGAAGACACAGAA
  GGGAACCCCTTGCTGCTGGACTTGTGAGCCCTGTGATGGATACCAGTATCAATTTGATGAGATGACATGT
  CAGCATTGTCCCTATGACCAGAGGCCCAATGAGAACCGAACTGGCTGTCAGAACATCCCAATCATCAAAC
  TGGAGTGGCACTCCCCCTGGGCTGTAATTCCTGTCTTCCTGGCAATGTTGGGGATCATTGCCACCATCTT
  TGTCATGGCAACCTTCATCCGCTACAATGATACACCCATCGTCAGGGCATCTGGGCGGGAACTCAGCTAT
  GTTTTATTGACAGGCATCTTTCTCTGCTATATCATCACCTTCCTAATGATTGCCAAGCCAGATGTGGCAG
  TGTGTTCCTTCCGACGTGTCTTCTTGGGGTTGGGTATGTGTATTAGTTATGCAGCCCTTTTAACAAAAAC
  CAATCGGATTTATCGCATATTCGAGCAGGGCAAGAAATCGGTGACAGCTCCCAGACTCATAAGCCCAACA
  TCACAACTGGCAATTACCTCCAGTTTAATATCGGTGCAACTTCTAGGTGTCTTTATTTGGTTTGGGGTTG
  ACCCACCCAACATCATCATAGACTACGATGAGCATAAGACAATGAACCCAGAACAAGCCAGGGGAGTTCT
  CAAATGTGACATCACAGACCTTCAGATCATTTGTTCTTTGGGATATAGCATTCTTCTCATGGTCACATGT
  ACTGTGTATGCCATCAAGACTCGGGGTGTACCAGAGAATTTTAATGAAGCCAAGCCCATTGGATTCACTA
  TGTACACGACTTGTATCGTATGGCTTGCCTTCATCCCAATATTTTTTGGCACAGCGCAATCAGCAGAAAA
  GCTCTACATACAAACCACCACGCTTACAATCTCCATGAACCTAAGTGCATCAGTGGCGCTGGGGATGCTA
  TACATGCCGAAAGTGTACATCATCATTTTCCACCCTGAACTCAATGTCCAGAAACGGAAGCGAAGCTTCA
  AGGCCGTAGTCACAGCAGCCACCATGTCATCGAGGCTGTCACACAAACCCAGTGACAGGCCCAATGGTGA
  GGCAAAGACAGAACTTTGTGAAAATGTAGACCCAAACAGCCCTGCTGCAAAAAAGAAGTATGTCAGTTAT
  AATAACCTGGTTATCTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_177328
• Insert Size: 2748 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_177328.3, NP_796302.2
• RefSeq Size: 4181 bp
• RefSeq ORF: 2748 bp
• Locus ID: 108073
• UniProt ID: Q68ED2
• Cytogenetics: 6 E3
• Gene Summary: G-protein coupled receptor for glutamate. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors, such as adenylate cyclase. Signaling inhibits adenylate cyclase activity (By similarity).[UniProtKB/Swiss-Prot Function]