Pcsk5 (NM_001163144) Mouse Untagged Clone

CAT#: MC222581

Pcsk5 (untagged) - Mouse proprotein convertase subtilisin/kexin type 5 (Pcsk5), transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Pcsk5
• Synonyms: b2b585C; b2b585Clo; b2b1549C; b2b1549Clo; PC; PC5; PC5/; PC6; SP; SPC6
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC222581 representing NM_001163144
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACTGGGACTGGGGGAACCGCTGCAGCCGCCCGGGACGGCGGGACCTGCTGTGCGTGCTGGCACTGC
  TCGCCGGCTGTCTGCTCCCGGTATGCCGGACGCGCGTCTACACCAACCACTGGGCAGTGAAGATCGCCGG
  CGGCTTCGCGGAGGCAGATCGCATAGCCAGCAAGTACGGATTCATCAACGTAGGACAGATCGGTGCACTG
  AAGGACTACTATCACTTCTACCATAGTAGGACCATTAAAAGGTCTGTTCTCTCGAGCAGAGGAACCCACA
  GTTTCATTTCAATGGAACCAAAGGTGGAGTGGATCCAACAGCAAGTGGTGAAAAAAAGAACCAAGAGGGA
  TTATGACCTCAGCCATGCCCAGTCAACCTACTTCAATGATCCCAAGTGGCCAAGTATGTGGTACATGCAC
  TGCAGTGACAACACCCATCCTTGCCAGTCAGACATGAATATCGAAGGAGCCTGGAAGAGAGGCTACACGG
  GGAAGAATATCGTGGTGACTATCCTGGATGACGGCATCGAGAGAACCCACCCAGATCTGATGCAAAACTA
  CGATGCTCTGGCAAGTTGCGATGTGAATGGGAATGACTTGGACCCGATGCCTCGTTATGATGCAAGCAAT
  GAGAACAAGCATGGGACCCGCTGTGCCGGAGAAGTGGCAGCCACTGCAAACAACTCTCACTGCACTGTCG
  GGATCGCTTTCAACGCCAAGATTGGAGGTGTGAGAATGCTGGATGGTGATGTCACTGACATGGTGGAGGC
  AAAGTCTGTCAGCTACAACCCACAGCATGTGCACATCTACAGTGCCAGCTGGGGACCAGATGACGATGGC
  AAGACTGTGGATGGGCCAGCTCCCCTCACCCGGCAAGCCTTTGAGAACGGCGTGAGAATGGGGCGGAGAG
  GCCTTGGATCTGTGTTTGTGTGGGCATCTGGCAATGGTGGACGGAGCAAGGATCACTGTTCTTGTGATGG
  CTATACCAACAGCATCTATACCATCTCCATCAGCAGTACGGCCGAAAGTGGAAAGAAACCTTGGTACTTG
  GAAGAGTGTTCATCTACACTGGCTACAACCTACAGCAGTGGAGAATCCTATGATAAGAAAATAATCACTA
  CTGATCTAAGGCAGCGATGCACAGACAATCACACTGGAACATCAGCCTCAGCCCCCATGGCTGCTGGAAT
  CATTGCCCTGGCCCTAGAAGCCAATCCGTTTCTGACCTGGAGAGACGTGCAGCATGTTATTGTCAGGACT
  TCCCGTGCGGGACATTTGAACGCTAATGACTGGAAAACCAATGCTGCTGGTTTTAAGGTGAGCCATCTCT
  ATGGATTTGGACTGATGGATGCCGAAGCCATGGTGATGGAAGCAGAGAAGTGGACAACTGTTCCTCAGCA
  GCACGTGTGTGTGGAAAGCACAGACCGACAAATCAAGACCATTCGACCAAACAGTGCAGTGCGCTCCATC
  TACAAAGCCTCAGGCTGCTCGGATAATCCCAACCATCACGTCAATTACCTGGAGCATGTAGTTGTGCGTA
  TTACCATCACACACCCACGGAGGGGAGACCTGGCCATCTATCTGACATCACCCTCAGGAACCAGATCCCA
  GCTCTTGGCCAACAGGCTCTTTGATCATTCCATGGAAGGGTTTAAGAACTGGGAGTTCATGACTATTCAT
  TGCTGGGGAGAACGGGCTGCTGGGGACTGGGTCCTCGAAGTTTATGATACGCCATCTCAGCTGAGGAACT
  TCAAGACTCCAGGTAAATTGAAAGAATGGTCCTTAGTCCTCTATGGCACGTCCGTACAGCCATACTCCCC
  AACCAACGAGTTTCCCAAAGTGGAACGCTTCCGCTACAGCCGAGTGGAAGACCCCACAGATGACTACGGT
  GCTGAAGATTATGCAGGTCCCTGTGACCCTGAATGCAGTGAGGTTGGATGTGACGGGCCAGGACCAGATC
  ACTGCAGTGACTGCTTACACTACTACTACAAGCTGAAAAATAACACCAGAATCTGTGTCTCCAGCTGCCC
  TCCTGGCCACTACCATGCTGACAAGAAGAGATGCCGGAAGTGTGCCCCAAACTGCGAGTCCTGCTTTGGC
  AGCCATGGTAATCAGTGCCTCTCCTGTAAATATGGCTACTTCCTGAATGAAGAAACTAGCAGCTGTGTTA
  CTCAGTGCCCTGATGGATCATACGAGGATATCAAGAAAAATGTCTGTGGGAAATGCAGTGAGAACTGCAA
  GGCATGCATTGGATTTCACAACTGCACAGAGTGCAAGGGCGGGTTAAGTCTTCAGGGATCCCGCTGTTCG
  GTCACCTGCGAGGATGGACAGTTCTTCAATGGTCACGACTGCCAGCCCTGCCATCGCTTCTGTGCTACTT
  GCTCTGGGGCCGGAGCAGATGGATGTATTAACTGCACCGAGGGGTATGTCATGGAAGAGGGAAGGTGTGT
  ACAAAGTTGTAGTGTGAGCTACTACTTGGACCACTCTTCAGAGGGTGGCTACAAATCCTGCAAGAGATGT
  GATAACAGCTGTTTGACATGCAATGGGCCAGGATTCAAGAACTGTTCCAGCTGCCCCAGTGGATATCTTT
  TAGACTTAGGAACGTGTCAGATGGGAGCCATCTGCAAGGATGCTACGGAAGAGTCCTGGGCAGAAGGAGG
  CTTCTGTATGCTTGTGAAAAAGAACAATCTCTGTCAGCGAAAAGTTCTACAACAGCTGTGCTGCAAAACA
  TGTACATTCCAAGGCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001163144
• Insert Size: 2748 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001163144.1, NP_001156616.1
• RefSeq Size: 5783 bp
• RefSeq ORF: 2748 bp
• Locus ID: 18552
• Cytogenetics: 19 12.86 cM
• Gene Summary: This gene encodes a subtilisin-like proprotein convertase that mediates posttranslational endoproteolytic processing of various proprotein substrates traversing the secretory pathway. The encoded protein is an inactive zymogen that undergoes autoproteolytic processing in the endoplasmic reticulum and the Golgi network to generate an active enzyme. Mice lacking the encoded protein die at an early embryonic stage. Conditional inactivation this gene in the epiblast but not in the extraembryonic tissue bypasses embryonic lethality but results in death at birth. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2015]
  Transcript Variant: This variant (2, also known as PC6A) represents use of an alternate 3' coding region and 3' UTR, compared to variant 1. The resulting isoform (2) has a substantially shorter and distinct C-terminus, compared to isoform 1. This isoform (2) may undergo proteolytic processing similar to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.