Rnf216 (NM_207110) Mouse Untagged Clone

CAT#: MC222544

Rnf216 (untagged) - Mouse ring finger protein 216 (Rnf216), transcript variant B, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Rnf216
• Synonyms: 2810055G22Rik; AI647468; AU019462; C86502; F830018F18Rik; TRIAD3; Ubce7ip1; UIP83
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC222544 representing NM_207110
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAGAGGGAAACAACAAAGAGGAGGTCATTCACTTGAACAACTTTCCCTGCCACCGGGGAAAGGAAT
  GGATGGCTGTCAGAGAGGGGCCCATCACCATATCTGACTCCTCTGATGAGGAAGGGATTCCAATGCTGGT
  TACCCCAGCTACAGAGCAACAGGAAGACGACCTGGATGATGATGTCATCCTGACAGAAACACATAAACCT
  CAGACATCCCGGCCCAATCTCATCAAACCAGCTGCCCAGTGGCAAGATCTGAACAGATTGGGAGAAGAAA
  GGCCTAGAAAATCTAGAGCAGACTTTGAAGCAGATATCCACAACTATTTTTCCTTTTGTAACAACTCATT
  GTTTGGTTCTGGGGCACAGGATGATTCTGAGGATGAATATGGTGGATTTCTGGATCTTGAGAGTGGCAAA
  AAAGAGGGAGAAGCCAAACCTGGACCAAGCAGTAAGCAGACAGCAGACGATATTGTCAACCCCAGATTGG
  AGCAGAAAGTCATTATATTGGGAGAAAATGGTCTTCTTTTCCCAGAAAGTGAGCCTTTGGAAGTTCAGAA
  CCAATCATCCGAAGACTCAGAGACAGAGCTCTTATCAAATCCTGGAGAGCCAGCTGCCTCAGTAGATGAC
  CAGCTAATTGGAGAAGAGTACTGGCTTGACCATCCATACTTCCAGGCTCCGAATCCACAGCCTCAGGAAA
  GAACAAACCAGGTTGTGCCCCAAGAGCGACATTCTGAATCAGAAATGGGCCCAATGTTTTTCCGCCATGA
  TTTCCCAGAGCCAGCTTTTCCAAGGCCAGAACCCCAGCAGGAGGGGATTCCAGGCCCTGCTTCTCCACAG
  CCAGCCCATCCTCTAGGAGAGCTTGAAGACCAGCAGTTAGCAATTGATGAAGACCCTGGGCCAGCCTTCC
  CCCTGTCAGGACCTCAGGAGGCCAACTTGGCAAACATGTGGGAACAAGAAGCTGCTGAGGTAGATCAAGA
  CCTCATTCCACTGTTAGTGAAAGAAACGGAAGCAAGATTTCCAGATGTAGCAAGTGGATATGTTGAAGAA
  ATAATTCATTTGAAAAATTACTATGATTTGAATGTACTTTGTAATTTTCTTCTGGAAAACCCAGATTATC
  CAAAGAGAGAAGACCGGCTTATTATCCACCCCAGCAGCAGCCTGCTTGCCAGCCAGGATGACGCGAAGTT
  GCCTAAAATAGACTTTTTTGACTATTCCAAATTGACTCCACTTGACCAGCGCTGCTTCATCCAAGCTGCT
  GACCTCCTGATGGCTGATTTCAAGATGCTTAGCAGCCAGGACATCAAGTGGGCCCTTCATGAGCTCAAAG
  GACACTATGCAATCACCCGAAAGGCCTTTTCCGATGCCATTAAGAAATGGCAGGAGTTGTCACCTGAAAC
  CAGTGGAAAACGGAAAAAGAGGAAAGAAATGAATCAGTATTCTTTCATAGATTTCAAGTTTGAACAAGGA
  AACATAAAAATAGAAAAGAGGATGTTCTTTCTGGAAAACAAGCGTCGACACTGTAGGTACTATGACCATC
  AGGCCCTCCTTCCAGCTGTGAAACAGGAGCAGGAATTCTATGAGCAGAAAATTAAAGAGATGGCAGAGCA
  TGAAGACTTCTTGCTCGCCCTGCAGATGAATGAAGAACAGTATCAAAAGGATGGCCAGCTGATTGAATGC
  CGCTGTTGCTATGGGGAGTTTCCATTTGAGGAGCTGACACAATGCGCCGATGCTCACTTGTTCTGCAAAG
  AATGTCTCATCAGATATGCCCAGGAGGCAGTGTTTGGATCTGGAAAGTCAGAACTCAGCTGCATGGAAGG
  CAGCTGCACGTGCTCCTTCCCAACCAGTGAGCTGGAGAAGGTGCTCCCCCAGACCATTCTGTACAAATAC
  TATGAACGGAAAGCTGAGGAAGAAGTCGCTGCAGCCTATGCTGATGAGCTTGTCCGGTGCCCCTCCTGCA
  GCTTCCCTGCTCTGTTGGACAGCGATGTGAAGAGGTTCAGCTGCCCCAACCCACGCTGCCGAAAGGAGAC
  CTGTAGGAAGTGTCAGGGACTCTGGAAAGAACACAACGGCCTCACCTGTGAAGAGCTGGCAGAAAAGGAT
  GACATCAAGTACCGGACATCCATTGAAGAAAAAATGACTGCTGCTCGGATTAGAAAATGTCACAAGTGTG
  GAACTGGCCTCATCAAGTCTGAAGGCTGCAACCGCATGTCTTGCCGCTGTGGTGCCCAGATGTGCTACCT
  CTGTCGAGTTTCTATCAATGGCTATGACCATTTCTGCCAGCATCCTCGCTCTCCAGGAGCCCCTTGCCAA
  GAATGTTCCAGGTGCTCCCTGTGGACCGACCCCACTGAAGATGATGAAAAGCTGATTGAGGAAATCCAAA
  AGGAGGCCGAGGAGGAACAGAAGAGAAAGAATGGAGAGAACACCTTCAAACGCATCGGGCCCCCACTGGA
  GAAGCCAGCTGAGAAGGTGCAGCGTGTGGAAGCCCTGCCGCGGCCTGTCCCACAGAACCTGCATCCTCAG
  ATGCCGCCCTATGCCTTTGTTCACCCACCCTTCCCCCTGCCACCTGTGCGGCCCGTGTTCAACAACTTCC
  CCATCAACATGGGTCCTGTGCCCGCTCCCTATGTCCCCCCTCTGCCCAACGTGCGTGTCAACTATGACTT
  TGGCCACATGCACGTGCCCCTGGAGCACAACCTGCCCATGCACTTTGGCCCCCAACCACGGCATCGCTTC
  TGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_207110
• Insert Size: 2733 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_207110.1, NP_996993.1
• RefSeq Size: 4692 bp
• RefSeq ORF: 2733 bp
• Locus ID: 108086
• UniProt ID: P58283
• Cytogenetics: 5 G2
• Gene Summary: Acts as an E3 ubiquitin ligase, which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes, and then transfers it to substrates promoting their degradation by the proteasome. Promotes degradation of TRAF3, TLR4 and TLR9. Contributes to the regulation of antiviral responses. Down-regulates activation of NF-kappa-B, IRF3 activation and IFNB production. Promotes TNF and RIP mediated apoptosis.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (B) represents the longest transcript and encodes the longest isoform (B).