Mcm2 (NM_008564) Mouse Untagged Clone

CAT#: MC222517

Mcm2 (untagged) - Mouse minichromosome maintenance deficient 2 mitotin (S. cerevisiae) (Mcm2), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Mcm2
• Synonyms: AA959861; AW476101; BM28; CDCL1; Mcmd2; mKIAA0030
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC222517 representing NM_008564
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGAGTCTTCTGAGTCTCTCTCAGCATCTAGCCCTGCCCGTCAGCGGCGCCGGATCAGTGATCCCC
  TCACCTCCAGCCCAGGCCGCAGCTCCAGACGTGCTGACGCCCTGACCTCCAGCCCTGGCAGAGACCTCCC
  CCCATTTGAAGATGAGTCTGAGGGGCTGCTGGGCACAGAGGGGCCCATGGAGGAAGAAGAGGATGGAGAG
  GAACTCATTGGTGATGGCATGGAGAGAGACTACCGTCCCATTCCGGAGCTCGATGTCTACGAGGCCGAGG
  GATTGGCCCTGGATGATGAAGATGTGGAGGAGCTGACAGCCAGTCAGAGAGAGGCAGCTGAGCGGACCAT
  GAGGCAGCGGGACCGTGAGGCTGGCAGAGGCCTGGGACGCATGCGCCGGGGGCTGCTCTATGACAGCAGC
  GAGGAAGATGAGGAGCGGCCTGCCCGTAAGCGCCGCCACGTAGAACGCGCCACAGAGGATGGCGAGGAGG
  ATGAAGAGATGATCGAGAGTATTGAGAATCTGGAGGACCTCAAGGGCCACTCGGTGCGCGAGTGGGTGAG
  CATGGCAGGGCCCAGGCTGGAGATCCACCACCGCTTCAAGAACTTCCTGCGCACCCACGTGGACAGCCAT
  GGCCACAACGTCTTCAAGGAGCGCATCAGTGATATGTGCAAAGAGAACCGTGAGAGTTTGGTGGTAAATT
  ATGAAGACCTGGCAGCCCGGGAGCACGTGTTGGCATACTTCCTGCCGGAAGCACCGGCTGAGTTGCTGCA
  GATCTTTGACGAGGCTGCCCTGGAGGTCGTGTTGGCCATGTACCCTAAATATGACCGTATCACCAACCAC
  ATCCATGTGCGCATCTCCCACCTGCCTCTGGTGGAGGAGCTGCGTTCACTGAGGCAGTTGCACCTGAACC
  AGCTGATCCGTACCAGTGGCGTGGTGACCAGCTGCACCGGAGTCCTGCCCCAGCTCAGCATGGTCAAGTA
  CAACTGTAGCAAGTGCAACTTTGTACTGGGGCCTTTCTGCCAGTCTCAGAATCAGGAGGTGAAGCCTGGC
  TCCTGCCCTGAGTGCCAGTCTGCTGGGCCCTTTGAGATCAACATGGAGGAGACCATCTATCAGAACTACC
  AACGTATCCGCATCCAGGAGAGTCCCGGCAAGGTGGCGGCTGGCCGACTGCCCCGTTCCAAGGATGCCAT
  TCTCCTCGCTGATCTGGTGGACAGCTGCAAGCCAGGGGACGAGATAGAGCTGACCGGCATTTACCATAAT
  AACTATGACGGCTCGCTTAACACCGCCAACGGCTTTCCAGTCTTTGCCACTATTATCTTGGCCAACCATG
  TTGCCAAGAAGGACAACAAAGTAGCTGTGGGGGAGCTCACCGATGAGGACGTGAAGATGATCACCGGTCT
  CTCCAAGGATCAGCAAATTGGAGAGAAGATCTTTGCCAGCATTGCACCCTCCATCTATGGGCATGAAGAC
  ATCAAGAGAGGCCTGGCTCTGGCCCTGTTTGGAGGGGAGCCCAAGAACCCAGGTGGAAAGCACAAGGTTC
  GAGGTGACATTAATGTGCTCTTGTGTGGGGACCCTGGCACAGCAAAGTCCCAATTCCTCAAATACATCGA
  GAAAGTGTCTAGCCGTGCCATCTTCACCACTGGCCAGGGTGCGTCAGCAGTGGGTCTCACCGCGTACGTT
  CAGCGGCATCCCGTCAGCAGAGAGTGGACCTTAGAGGCGGGAGCCCTGGTTCTGGCTGACCGGGGGGTGT
  GTCTCATTGACGAGTTTGACAAGATGAATGACCAGGACAGGACCAGCATCCACGAGGCCATGGAACAGCA
  AAGCATCTCCATCTCCAAGGCTGGCATCGTTACCTCGCTGCAAGCCCGCTGCACTGTCATAGCTGCTGCC
  AACCCCATAGGAGGCCGCTACGACCCTTCACTGACCTTCTCAGAGAATGTAGACCTCACAGAGCCCATCA
  TTTCCCGCTTTGATGTCCTGTGTGTGGTGAGGGACACTGTTGATCCAGTTCAGGATGAGATGCTGGCCCG
  CTTTGTGGTTGGCAGCCACGTCAGACACCACCCCAGTAACAAGAAGGATGAAGGGTTGACTAATGGTGGC
  ACCTTGGAGCCAGCCATGCCCAACACATATGGCGTGGAGCCCCTGCCTCAGGAGGTGCTGAAGAAGTATA
  TCATCTATGCCAAGGAGAGGGTCCGCCCGAAGCTCAACCAGATGGACCAGGATAAAGTGGCCAGGATGTA
  CAGTGACCTGAGGAAGGAGTCCATGGCAACGGGCAGCATTCCCATCACGGTGCGCCACATCGAGTCCATG
  ATCCGCATGGCCGAGGCCCATGCCCGCATGCACCTGCGGGACTACGTGATGGAAGACGATGTCAACATGG
  CCATCCGAGTGATGATGGAGAGCTTCATTGACACCCAGAAGTTCAGCGTCATGCGGAGTATGCGCAAGAC
  TTTTGCCCGGTATCTCTCCTTCCGGCGAGATAACAATGATCTGCTGCTCTTCATACTGAAGCAGTTGGTG
  GCTGAGCAGGTGACATATCAACGCAACCGCTTTGGGGCCCAGCAGGACACCATTGAAATACCTGAGAAGG
  ATCTGATGGACAAGGCCAGGCAGATCAATATTCACAACCTCTCTGCCTTCTACGACAGCGACCTCTTCAA
  ATTCAACAAGTTCAGCCGTGACCTGAAACGCAAACTGATCCTACAGCAGTTCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_008564
• Insert Size: 2715 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_008564.2, NP_032590.2
• RefSeq Size: 3381 bp
• RefSeq ORF: 2715 bp
• Locus ID: 17216
• UniProt ID: P97310
• Cytogenetics: 6 D1
• Gene Summary: Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for the entry in S phase and for cell division. Plays a role in terminally differentiated hair cells development of the cochlea and induces cells apoptosis.[UniProtKB/Swiss-Prot Function]