Ttll7 (NM_027594) Mouse Untagged Clone

CAT#: MC222427

Ttll7 (untagged) - Mouse tubulin tyrosine ligase-like family, member 7 (Ttll7), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Ttll7
• Synonyms: 1110049N09Rik; 4921517B04Rik; C630030B20; Ttll7s
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC222427 representing NM_027594
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCATCTCTGCCTCAAGATGGGGTTATTCAGGGATCATCTCCTGTGGATCTGGGTACAGAATTACCTT
  ACCAATGCACAATGAAAAGGAAAGTGAGAAAAAAGAAGAAGAAGGGAATTATTACAGCTAATGTTGCCGG
  AACAAAGTTTGAAATTGTTCGTTTAGTAATAGATGAGATGGGATTTATGAAAACTCCTGATGAAGATGAG
  ACAAGCAATCTTATCTGGTGTGATGCTGCTGTTCAGCAGGAGAAGATTACAGACCTGCAGAACTACCAGA
  GAATCAACCATTTTCCAGGAATGGGAGAGATCTGTAGGAAGGATTTCTTAGCAAGAAATATGACGAAAAT
  GATCAAGTCTCGGCCTATGGATTATACCTTTGTTCCGAGAACGTGGATTTTCCCTTCTGAATATACTCAG
  TTCCAAAACTATGTGAAAGAATTGAAGAAAAAACGGAAGCAGAAAACTTTTATAGTAAAACCAGCTAATG
  GTGCAATGGGTCATGGGATTTCATTGATAAGAAATGGGGATAAAGTCCCTTCTCAGGATCATTTGATAGT
  TCAAGAATACATTGAGAAACCTTTCCTAATGGAAGGCTACAAGTTTGACTTACGGATTTATATTTTGGTT
  ACATCCTGTGATCCATTAAAGATATTTCTCTACCATGATGGGCTTGTGCGCATGGGCACTGAGAAGTACA
  TCCCACCAAACGAGTCCAACTTGACCCAGTTATACATGCATCTGACCAACTACTCAGTGAACAAACACAA
  TGAACGGTTTGAGCGCAATGAAACTGAAGACAAAGGCAGCAAGCGGTCAATCAAGTGGTTTACGGAATTC
  CTACAGGCCAATCAACATGATGTCACTAAGTTCTGGAGTGATATTTCAGAGTTGGTGGTAAAGACTCTGA
  TTGTGGCAGAACCTCACGTCCTGCACGCCTACCGGATGTGCAGACCCGGTCAGCCCCCCGGGAGCGAAAG
  TGTCTGCTTCGAAGTCCTGGGGTTCGACATCTTGTTGGATAGAAAGCTCAAGCCATGGCTTCTGGAGATT
  AATCGAGCCCCAAGCTTTGGAACTGATCAGAAAATCGATTATGATGTTAAAAGGGGGGTTCTGCTAAACG
  CGCTGAAGCTGCTGAACATCAGGACCAGCGATAAAAGGAAAAACTTGGCCAAGCAAAAAGCGGAGGCTCA
  GAGGAGGCTGTACGGTCAAAACCCAGTAAGAAGGCTTTCGCCAGGCTCCTCCGACTGGGAACAGCAGAGG
  CACCAGCTGGAGAGGCGGAAAGAAGAGTTGAAAGAGAGACTCCTTCAAGTACGAAAGCAGGTTTCGCAGG
  AAGAACATGAAAACCGACATATGGGGAATTACAGGAGAATTTATCCTCCTGAAGACAAAGCACTGCTTGA
  GAAGTATGAAGGTTTGCTGGCCGTCGCCTTCCAGACCTTCCTCTCGGGAAGAGCAGCTTCATTCCAGCGA
  GAAATGAACAATCCTCTGAAAAAGATGCGGGAGGAAGATCTCCTGGATCTTCTGGAGCAGTGCGAAATCG
  ATGATGAAAAGCTGATGGGGAAAACCGGCCGGGTCCGAGGACCGAAGCCCCTGTGCTGCATGCCAGAGTG
  TGCAGAGGTCACGAAGAAGCAGAAGTATTACGGTAGCAGTGACAGCAGCTACGACAGCAGCAGCAGCAGC
  AGCAATTCAGAGCTGGATGAGAACGAAAAAGAGCTGTGTCAGAAGAGACTAGACCAAGTCCCATATAGCC
  TTAAGCACACCAGTCACTGCAAGATAATCCAGCAATCCAATTCCATGAGGCGTTCGGTCAGCTGCCCTCG
  GTCCATCTCTGCTCACTTACCTTCCAGAGGGGATGTGCGCCCCTTTTCTTCTCAACAAGTGATACCATTA
  GCAAGACCAACGTCAGCGTCCCGGTCACATTCCTTAAACCGCGCTTCATCCTACGCGAGACACCTGCCTC
  ACGGCAGTGACACCGGCTCTACCAACACTCTGGTAAGCTATCAGTGGAACGAGTCTTTGCGGCAATTGAA
  AACCAAAGAGCAGGAAGACGACCTCACCAGTCAGACCTTGTTTGTCCTCAAGGACATGAGAATCCGCTTT
  CCGGGGAAGTCAGACGCAGAGTCGGAGCTCCTGATAGAAGATATCATGGATAACTGGAAGCATTATAAAA
  CAAAAGTGGCTTCATATTGGCTCATAAAATTGGACTCTGTAAAACAACGGAAAGTTTTGGACATAGTAAA
  ATCAAGTATTCGTACAGTTCTTCCAAGAATCTGGAGGGTCCCTGATGCTGAAGAACTGAGTTTATACCGG
  ATTTTCAACAGGGTATTTAATCGCCTACTCTGGAGTCATGGCCAAGGACTGTGGAGCTGTTTCTGTGATT
  CAGGATCCTCTTGGGAGAGCATCTTCAGTAAGAGCCCTGAGGTGGTGACCCCTCTGCAGCTCCAGTGCTG
  CCAGCGCCTGGTGGAGCTTTGCAAGCAGTGCCTGCTGGTGGTTTACAAATACACAACAGAAACAAGAGGA
  CCCATCTCAGGCATCGGTCCTGACTGGGGGAACTCCAGGTATTTGCTACCAGGAAGTACCCAATTTCTTA
  TGAGATCACCACTGTACAACATGAAATATAATTCACCCGGAATGACTCGCTCCAATGTTTTGTTTACGTC
  CCGCTATGGCCGTTTGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_027594
• Insert Size: 2679 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_027594.1, NP_081870.1
• RefSeq Size: 6075 bp
• RefSeq ORF: 2679 bp
• Locus ID: 70892
• Cytogenetics: 3 H2
• Gene Summary: Polyglutamylase which preferentially modifies beta-tubulin (PubMed:16901895, PubMed:17499049, PubMed:19152315). Mediates both ATP-dependent initiation and elongation of polyglutamylation of microtubules (PubMed:16901895, PubMed:19152315). Required for neurite growth; responsible for the strong increase in tubulin polyglutamylation during postnatal neuronal maturation (PubMed:16901895).[UniProtKB/Swiss-Prot Function]