Eef1akmt4-ece2 (NM_177940) Mouse Untagged Clone

CAT#: MC222391

Ece2 (untagged) - Mouse endothelin converting enzyme 2 (Ece2), transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Eef1akmt4-ece2
• Synonyms: 1810009K13Rik; 6330509A19Rik; 9630025D12Rik; BB127715; Ece-2; Eef1akmt4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC222391 representing NM_177940
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCTCTCCCCGGACTCCGGTGTCCCCTCCGGAGTTACCGGAGAAGAACTTCCAGTACCGCCAAGTCC
  AGTACTGGGACCAGCGCTACAAAGACGCGGCCGACTCTGGACCCTACGAGTGGTTCGGAGACTTCGCTTC
  CTTCCGTGCCCTCCTAGAGCCGGAGCTGTGTCCTGAGGACCGTATCCTCGTGCTAGGTTGCGGGAACAGT
  GCCTTGAGCTATGAGCTGTTCCTTGGAGGCTTCCCTAATGTGACCAGTGTGGATTACTCTCCAGTAGTGG
  TGGCAGCCATGCAAGTTCGCTATGCCCATGTACCCAGTCTACGCTGGGAGACCATGGATGTTCGAGCACT
  GGACTTCCCTAGTGGGTCTTTTGATGTTGTCCTCGAGAAGGGTACTCTGGATGCCATGCTGGCTGGGGAG
  CCAGATCCCTGGAATGTATCTTCTGAAGGTGTCCACACTGTGGACCAGGTGCTGAGTGAGGTGGGATTCC
  AGAAGAGGACAAGACAACTGTTTGGTTCACACACACAGTTGGAGCTGGTCTTGGCAGGCCTCATTCTAGT
  GTTGGCTGCCCTTCTTTTGGGCTGCCTCGTGGCTCTGTGGGTCCACAGAGACCCAGCCCATAGCACCTGC
  GTCACAGAAGCCTGCATTCGAGTAGCTGGAAAAATCCTGGAGTCTCTAGACCGTGGGGTGAGCCCCTGTC
  AGGACTTTTACCAGTTCTCCTGTGGAGGCTGGATTCGAAGAAACCCTCTACCCAATGGACGTTCTCGCTG
  GAACACCTTCAACAGCCTCTGGGACCAGAACCAGGCCATACTGAAGCACCTACTTGAGAACACCACTTTC
  AATTCCAGCAGTGAAGCTGAGAGGAAGACTCGGAGTTTCTACCTGTCCTGCCTACAGTCGGAGCGCATTG
  AGAAGCTAGGAGCCAAGCCACTTAGAGACCTCATTGACAAGATCGGTGGTTGGAACATAACGGGGCCTTG
  GGACGAGGACAGCTTCATGGATGTGCTCAAGGCAGTCGCAGGGACCTACAGAGCCACCCCCTTCTTCACC
  GTCTACGTCAGTGCTGATTCTAAGAGTTCTAACAGCAATATCATCCAGGTGGACCAGTCTGGGCTTTTTC
  TGCCCTCTCGAGATTACTACCTAAATAGAACTGCCAATGAGAAAGTTCTCACTGCCTACCTGGACTACAT
  GGTGGAGCTGGGAGTGCTGCTGGGTGGACAGCCGACCTCCACTCGGGAGCAGATGCAGCAGGTGCTGGAG
  CTGGAGATACAGCTGGCTAACATCACTGTGCCCCAGGACCAGCGGCGTGATGAGGAGAAGATCTATCACA
  AGATGAGCATCTCAGAGCTGCAGGCTCTCGCGCCCGCCGTGGACTGGCTGGAGTTCCTTTCTTTCTTGTT
  ATCGCCACTTGAGTTGGGTGATTCTGAGCCTGTGGTGGTGTATGGGACTGAGTATTTACAGCAGGTGTCG
  GAGCTCATCAACCGTACTGAACCAAGCATCCTGAACAATTACCTAATTTGGAACCTGGTACAGAAGACGA
  CCTCAAGCCTTGACCAGCGCTTTGAGACTGCACAGGAGAAACTGCTGGAGACCCTCTACGGTACCAAGAA
  GTCCTGCACTCCGAGGTGGCAGACCTGCATCTCCAATACAGATGATGCCCTTGGCTTTGCTCTGGGTTCA
  CTCTTTGTGAAAGCCACATTTGACCGACAAAGCAAGGAAATCGCCGAGGGGATGATCAATGAAATCCGCT
  CTGCTTTTGAGGAGACCCTGGGAGACTTGGTTTGGATGGATGAGAAGACCCGGCTGGCAGCCAAGGAGAA
  AGCAGATGCCATCTATGATATGATTGGTTTCCCTGATTTCATCCTGGAGCCCAAAGAGCTGGATGATGTT
  TATGATGGGTATGAAGTCTCTGAAGATTCTTTTTTCCAAAACATGTTGAATCTGTACAACTTCTCAGCTA
  AGGTGATGGCTGACCAGCTCCGCAAACCTCCCAGCCGAGACCAGTGGAGCATGACACCTCAGACCGTGAA
  CGCTTACTACCTTCCAACCAAGAATGAAATCGTCTTCCCTGCTGGCATCTTGCAGGCCCCCTTCTATGCT
  CACAACCATCCAAAGGCCTTGAACTTTGGTGGCATCGGCGTAGTGATGGGCCATGAGTTGACACATGCCT
  TTGATGACCAAGGGCGTGAGTATGACAAAGAAGGGAATCTGCGTCCTTGGTGGCAGAATGAGTCACTGAC
  GGCTTTCCAGAACCATACAGCCTGCATGGAAGAACAGTACAGCCAGTACCAGGTCAATGGAGAGAGGCTC
  AATGGACTCCAGACCCTGGGGGAAAACATCGCCGATAATGGGGGCCTTAAGGCTGCTTACAATGCTTACA
  AAGCATGGCTGAGAAAGCATGGGGAGGAGCAGCCGCTGCCTGCTGTGGGGCTCACCAATCACCAGCTTTT
  CTTCGTGGGATTTGCTCAGGTGTGGTGCTCGGTCCGCACACCAGAGAGCTCTCACGAGGGGCTGGTGACC
  GACCCCCACAGCCCTGCCCGTTTCCGAGTGCTGGGCACTCTCTCCAACTCCCGAGACTTCCTTCGGCACT
  TCGGCTGCCCTGTCGGCTCCCCCATGAACCCAGGGCAGCTATGTGAGGTGTGGTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_177940
• Insert Size: 2646 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_177940.1, NP_808809.1
• RefSeq Size: 3349 bp
• RefSeq ORF: 2646 bp
• Locus ID: 110599584
• UniProt ID: P0DPE0
• Cytogenetics: 16 B1
• Gene Summary: This locus represents naturally occurring readthrough transcription between the adjacent genes eukaryotic translation elongation factor 1 alpha lysine specific methyltransferase 4 (GeneID:110599566) and endothelin converting enzyme 2 (GeneID:107522). The readthrough transcript representing this gene encodes a fusion protein that shares sequence identity with each individual gene product. [provided by RefSeq, Jul 2017]
  Transcript Variant: This variant (2, also known as 2a-1) lacks an alternate in-frame exon compared to variant 1. The resulting isoform (b, also known as 2a-1) has the same N- and C-termini but is shorter compared to isoform a.