Ptgfrn (NM_011197) Mouse Untagged Clone

CAT#: MC222374

Ptgfrn (untagged) - Mouse prostaglandin F2 receptor negative regulator (Ptgfrn), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Ptgfrn
• Synonyms: 4833445A08Rik; AU042434; CD9P-1; FPRP; Trim45
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC222374 representing NM_011197
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGCGCCCGGCGCCGAGGCCGCTGCTGCTGGCGCTCCTGTCGCTGGCTGTCTGCCGTGGGCGTGTGG
  TGAGAGTCCCTGCGGGCACCCTGGTTCGCGTGGTGGGCACTGAGCTGGTGATCCCCTGCAATGTCAGCGA
  CTATGACGGCCCCAGCGAGCAGAACTTCGACTGGAGCTTCTCGTCTTCCGGGAGCAGTTTCGTGGAGCTT
  GCTAGCACCTGGGAGGTGGGCTTCCCGGCACAGCTGTACCGGGAGCGGCTGCAGCGGGGTGATATCCTGT
  TGAGGAGAACTGCCAACGATGCTGTGGAACTGCACATAAAGAATGTCCAGCCCTCAGACCAAGGCCACTA
  CAAGTGTTCCACCCCGAGCACGGATGCCACTGTCCAGGGCAACTACGAGGACACAGTGCAGGTTAAAGTG
  CTGGCAGATGCCCTGGTGGTGGGTCCCAGCTCCCGGCCTCCTCCAGGCCTGAGCCTGCGTGAGGGGGAGC
  CCTTTGAGCTGCGATGTATCGCGTCTACCACGTCACCGTTGCACACACACCTGGCACTTCGGTGGGAACT
  GCACCGTGGCCCGGTGCACCGAAGCATCCTAGCCCTAAGCCACGAGGGCAGGTTTCACCCAGGCCCTGGC
  TATGAGCAGCGCTACCACAGTGGGGACGTACGCCTGGACACAGTAGGCAGCGATGCCTACCGCCTCTCCG
  TAGCCCGGGCGCTCTCTGCGGACCAGGGTTCCTATAGATGTGTGGTCAGCGAGTGGATCACAGAGCAGGG
  CAGCTGGCAAGAAATCCAAGAAAAAGCCGTGGAAGTGGCCACTGTGGTGATCCAGCCGACAGCTCTGCAA
  CTGGCCGTGCCCAGGACAGTGTCTGTGACTGAAGGAAAGGACCTGGATCTTTCTTGCAACATCACAACAG
  ACCGTGTGGATGATGTCCGGCCTGAGGTGACATGGTACTTCAAAAAGACACCCGATACCTCTTTACTTGC
  CTCCCACATGCTGGCGCGGCTTGACCGTGATTCCCTGGTCCACAGCTCACCACACGTTGCTCTCAGTCAC
  GTGGATACCCGCTCTTACCATCTACTGGTGCGAGATGTTAGCAAAGAAAACTCGGGCTACTATCTTTGCC
  TTGTGGCCCTCTGGGCTCCTGGACACAACAGGAGCTGGCACAAGGTGGCAGAGGCCATGTCTGCCCCGTC
  TGGTGTAAGCGTGACCTGGCTAGAACCAGAATACCAGGTCTACCTGAACGCTTCTAAGGTCCCCGGGTTT
  TCAGACGACCCCACGGAACTGCAGTGCCGGGTGATAGACACGAAGCGTCTGGAGGCCGGTGTCCGACTTA
  CCGTGTCGTGGTACTATAGAATGACCCGTCGCAATGACGACGTGGTGGCCAGTGAGCTTCTCGCAGTCAT
  GGATGGGGACTGGACTCTGAGATATGGAGAGAGGAGCAAGCAGCGGGCCCAGGACGGAGAGTTTATTTTT
  TCTAAGGAGCACACAGACACATTCAATTTCCGGATCCAAAGGACTACGGAGGAAGACAGGGGCAATTATT
  ACTGTGTTGTGTCTGCCTGGACCAGGCAGAGGAACAACAGCTGGGTGAAGAGCAAAGATGTCTTCTCCAA
  GCCTGTCAACATATTCTGGGCCTCAGAAGATTCTGTGCTCGTGGTGAAGGCACGGCAGCCAAAGCCTTTC
  TTTGCTGCAGGGAATACATTTGAGATGACTTGCAAAGTGTCTTCCAAGAATATTAAGTCGCCACGATACT
  CAGTTCTCATCACGGCTGAGAAGCCCGTCGGGGACCTCTCCAGTCCCAACGAGACCAAGTACATTATCTC
  CCTGGACCAGGACTCCGTGGTGAAGCTGGAGAACTGGACCGACGCGTCTCGGGTGGATGGCGTGGTGTTG
  GAGAAAGTTCAGGAGGATGAGTTCCGATACCGAATGTACCAGACTCAGGTCTCCGATGCGGGCCTGTACC
  GCTGCATGGTGACAGCCTGGTCTCCTATTGGGGGCAGCTTGTGGCGAGAGGCAGCGACCAGTCTCTCCAA
  TCCTATTGAGATCGACTTCCAAACCTCAGGTCCCACATTTAACGCCTCTGTGCATTCAGACACACCATCC
  GTCACCCGGGGAGATCTCATCAAATTGTTCTGTATCGTCACTGTGGAAGGAGCTGTGCTGGACCCAGATG
  ACATGGCCTTCGATGTATCCTGGTTTGCGGTACACTCTTTTGGCTTGGACAAGGCTCCCGTCCTCCTCTC
  CTCCTTGGACCGGAAGGGAGTCGTGACGACAGGCCAGAGGGACTGGAAGAGTACTGTCAGCCTGGAGCGA
  GTGAGCGTGCTGGAATTCTTGCTGCAAGTGCATGGCTCTGAGGACCAGGACTTTGGCAACTACTACTGTT
  CTGTGACTCCCTGGGTGAGGTCACCGACAGGTTCCTGGCAGAGGGAAGCCGAGATCCACTCCAGGCCCAT
  CTTTATAACTGTGAAGATGGACGTGCTGAACGCCTTCAAGTACCCGCTGCTGATCGGCGTGGGCCTGTCC
  ACAGTCATCGGGCTCCTGTCCTGCCTCATCGGATACTGCAGTTCCCATTGGTGCTGTAAGAAGGAGGTTC
  GGGAGACGCGTCGGGAGCGCCGCAGGCTCATGTCCATGGAGATGGATTAAAGCGGACCG
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-RsrII
• ACCN: NM_011197
• Insert Size: 2649 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_011197.3, NP_035327.2
• RefSeq Size: 5786 bp
• RefSeq ORF: 2640 bp
• Locus ID: 19221
• UniProt ID: Q9WV91
• Cytogenetics: 3 F2.2
• Gene Summary: Inhibits the binding of prostaglandin F2-alpha (PGF2-alpha) to its specific FP receptor, by decreasing the receptor number rather than the affinity constant. Functional coupling with the prostaglandin F2-alpha receptor seems to occur (By similarity). In myoblasts, associates with tetraspanins CD9 and CD81 to prevent myotube fusion during muscle regeneration.[UniProtKB/Swiss-Prot Function]