Aak1 (NM_177762) Mouse Untagged Clone

CAT#: MC222351

Aak1 (untagged) - Mouse AP2 associated kinase 1 (Aak1), transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Aak1
• Synonyms: 5530400K14Rik; 9630042K20; AU067724; AU067726; BC028270; C79663; D6Ertd245e; mKIAA1048; R75501
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC222351 representing NM_177762
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGAAGTTTTTCGACTCCAGGCGGGAGCAGGGGAGCTCTGGCCTGGGTTCAGGCTCCAGCGGAGGAG
  GGGGCAGCAGCTCAGGCCTGGGCAGTGGTTACATCGGAAGGGTCTTCGGCATCGGGAGGCAGCAGGTCAC
  CGTGGACGAGGTGTTGGCGGAAGGTGGATTCGCTCTTGTCTTTCTGGTGAGAACAAGCAATGGGGTGAAA
  TGTGCCTTGAAACGTATGTTTGTCAACAATGAACATGACCTCCAGGTGTGCAAGAGGGAGATCCAGATCA
  TGAGAGACCTATCAGGGCACAAGAACATTGTGGGCTACATTGATTCTAGTATCAACAATGTGAGCAGCGG
  CGACGTCTGGGAAGTTCTCATTCTCATGGACTTCTGCAGAGGAGGCCAGGTGGTAAACCTGATGAACCAG
  CGTCTCCAGACAGGCTTCACAGAGAATGAAGTGCTGCAGATATTCTGTGACACCTGTGAAGCTGTGGCCC
  GCCTGCATCAGTGCAAAACTCCCATTATCCACCGGGACCTGAAGGTTGAAAATATCCTCTTGCATGACCG
  AGGCCACTATGTCTTGTGTGACTTTGGAAGTGCCACCAACAAATTCCAGAATCCACAGGCCGAGGGAGTC
  AATGCAGTAGAAGATGAGATTAAGAAATACACAACGCTCTCCTATCGAGCCCCAGAAATGGTCAACTTGT
  ACAGTGGCAAAATCATCACTACGAAGGCAGATATTTGGGCTCTAGGCTGTTTGTTGTATAAATTATGCTA
  CTTCACTTTGCCGTTTGGGGAGAGCCAGGTGGCGATTTGTGACGGAAGCTTCACAATTCCTGATAACTCT
  CGTTATTCTCAAGATATGCACTGCCTTATTAGGTATATGTTGGAACCAGACCCTGACAAAAGGCCGGATA
  TTTACCAGGTTTCATACTTCTCATTTAAACTACTCAAGAAAGAATGCCCCGTTCCAAATGTACAGAACTC
  TCCCATTCCTGCAAAACTTCCTGAACCAGTGAAAGCCAGTGAAGCAGCTGTGAAAAAGACCCAGCCAAAG
  GCCAGACTGACCGATCCCATTCCCACCACAGAGACTTCAATCGCACCCCGCCAGAGGCCTAAAGCTGGGC
  AGACTCAGCCAAACCCAGGCATCCTTCCCATCCAGCCAGCCCTGACTCCTCGGAAGAGGGCCACTGTTCA
  GCCCCTACCTCAGGCTGCAGGACCCAGCAATCAGCCTGGACTTCTACCCAGTGTTTCCCAACCTAAAGCC
  CAAGCCACACCCAGTCAACCTCTTCAGTCATCTCAACCCAAGCAACCACAAGCTCCACCCACCCCACAGC
  AGACACCTGCTACCCAGACACAAGGTTTGCCCACCCAGGCCCAGGCCACTCCCCAGCACCAGCAGCAACA
  CCTCCTCAAGCAACAGCAGCAACAGCAGCAACAGCCTCAGCAGCCTACAGCACCACCACAGCCTGCAGGC
  ACCTTTTACCAGCAGCAGCAGCAACAGCAGCAGCAGCAGGCTCAGACTCAGCAGCAGATTCAAGCCCCAG
  TGAGACAACAGCCAAAAGTTCAGACCACTCCACCTCCAACCATCCAGGGACAGAAAGTTGGATCTCTCAC
  TCCTCCATCATCACCCAAAACCCAACGTGCTGGGCACAGACGGATTCTCAGTGATGTAACCCACAGTGCA
  GTCTTTGGGGTTCCTGCCAGCAAATCAACCCAGCTGCTCCAGGCAGCTGCAGCTGAGGCCAGTCTCAATA
  AATCTAAGTCTGCAACCACCACTCCCTCAGGCTCTCCACGGACTTCACAGCAGAATGTCTCCAATGCTTC
  AGAAGGCTCCACATGGAATCCTTTTGATGACGACAACTTCTCCAAACTCACAGCTGAAGAGCTGCTAAAC
  AAGGACTTTGCCAAGCTTGGGGAAGGAAAGCTGCCTGAGAAGCTTGGTGGCTCTGCAGAGAGTTTGATCC
  CAGGCTTCCAGCCAACACAAGGAGATGCCTTCACCACTCCTTCTTTTTCTGCTGGAACTGCTGAAAAAAG
  GAAGGGGGGCCAGGCTGTGGACTCTGGCATCCCACTTCTAAGTGTGTCTGATCCCTTCATTCCTCTTCAA
  GTACCTGATGCTCCAGAAAAACTAATCGAGGGACTCAAATCTCCTGACACTTCTCTTCTGCTCCCTGACC
  TCTTGCCTATGACAGATCCTTTTGGCAGCACTTCTGATGCTGTTATTGACAAAGCTGATGTTGCTGTTGA
  GAGTCTCATACCTGGACTGGAGCCACCTGTTGCTCAGCGCCTCCCATCTCAAACGGAATCTGTGACCTCG
  AACCGCACAGACTCTCTCACGGGGGAAGACTCTCTGCTTGACTGCTCTCTGCTTTCTAACCCTACTGCTG
  GCCTTCTGGAGGAGTTTGCCCCCATAGCACTCTCTGCTCCCACTCATAAAGCTGCAGAAGATAGCAATCT
  CATCTCAGGTTTTGGTGTTGCTGAGGGCTCCGAAAAGGTGGCAGAAGATGAGTTTGACCCTATTCCTGTA
  CTAATTACCAAAAACACACAAGGTGGGCACTCTAGGAACAGCAGTGGGAGCTCTGAGTCCAGTCTTCCCA
  ACCTAGCCAGGTCTTTGCTGCTGGTGGATCAGCTCATAGACCTGTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_177762
• Insert Size: 2637 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_177762.6, NP_808430.2
• RefSeq Size: 19120 bp
• RefSeq ORF: 2637 bp
• Locus ID: 269774
• UniProt ID: Q3UHJ0
• Cytogenetics: 6 37.75 cM
• Gene Summary: Regulates clathrin-mediated endocytosis by phosphorylating the AP2M1/mu2 subunit of the adaptor protein complex 2 (AP-2) which ensures high affinity binding of AP-2 to cargo membrane proteins during the initial stages of endocytosis. Isoform 1 and isoform 2 display similar levels of kinase activity towards AP2M1. Regulates phosphorylation of other AP-2 subunits as well as AP-2 localization and AP-2-mediated internalization of ligand complexes. Phosphorylates NUMB and regulates its cellular localization, promoting NUMB localization to endosomes. Binds to and stabilizes the activated form of NOTCH1, increases its localization in endosomes and regulates its transcriptional activity (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) lacks an alternate exon in the 3' CDS, compared to variant 1. The resulting protein (isoform 2) is shorter when compared to isoform 1.