Tlr5 (NM_016928) Mouse Untagged Clone

CAT#: MC222323

Tlr5 (untagged) - Mouse toll-like receptor 5 (Tlr5), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Tlr5
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC222323 representing NM_016928
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATGCTGAGTTCCCCCACGCACCACACTTCAGCAGGATCATGGCATGTCAACTTGACTTGCTCATAG
  GTGTGATCTTCATGGCCAGCCCCGTGTTGGTAATATCTCCCTGTTCTTCAGACGGCAGGATAGCCTTTTT
  CCGAGGCTGTAACCTCACCCAGATTCCCTGGATCCTCAATACTACCACTGAGAGGCTCCTGCTCAGCTTC
  AACTATATCAGTATGGTGGTTGCCACATCATTTCCACTCCTGGAGCGGCTCCAGTTGCTGGAGCTGGGGA
  CCCAGTATGCTAACTTGACCATTGGTCCAGGGGCTTTCAGAAACCTGCCCAATCTTAGGATCTTGGACTT
  GGGCCAAAGCCAGATCGAAGTCTTGAATCGAGATGCCTTTCAAGGTCTGCCCCATCTCTTGGAACTTCGG
  CTGTTTTCCTGTGGACTCTCCAGTGCTGTGTTAAGTGACGGTTACTTCAGAAATCTATATTCATTAGCTC
  GCTTAGACCTATCTGGCAACCAGATTCACAGCCTCCGCCTCCATTCTTCATTCCGGGAACTGAATTCCTT
  AAGCGACGTAAATTTTGCTTTCAACCAAATATTCACTATATGTGAAGATGAACTCGAGCCTCTGCAGGGC
  AAAACACTGTCTTTCTTTGGCCTCAAATTAACTAAGCTGTTCAGCAGAGTCTCTGTGGGCTGGGAGACAT
  GCAGGAACCCCTTCAGAGGCGTGAGGCTAGAAACTCTAGATCTTTCTGAAAATGGCTGGACGGTGGACAT
  CACAAGGAACTTCAGCAACATCATCCAGGGAAGCCAGATTTCCTCTTTGATTCTTAAACACCACATCATG
  GGTCCTGGCTTTGGCTTCCAGAACATCAGAGATCCTGACCAGAGCACATTTGCCAGCCTGGCCAGAAGTT
  CGGTGCTGCAACTGGACCTTTCGCACGGCTTTATCTTCTCCTTGAATCCTCGACTGTTTGGGACACTGAA
  GGATTTGAAGATGCTGAACCTTGCCTTCAACAAGATAAACAAGATTGGAGAGAATGCCTTTTATGGGCTT
  GACAGCCTCCAGGTTCTCAATCTATCCTATAATCTTTTGGGGGAACTCTATAATTCCAACTTCTATGGGC
  TTCCTAGAGTAGCCTACGTTGACCTTCAAAGGAACCACATTGGGATCATTCAAGACCAAACATTCAGATT
  ATTAAAAACGTTACAAACCTTAGATCTCCGTGACAATGCTCTTAAGGCCATTGGTTTTATTCCAAGCATA
  CAGATGGTCCTCCTGGGAGGCAATAAGCTGGTCCATTTGCCACACATCCACTTTACTGCCAACTTCCTAG
  AGTTATCTGAAAACAGGCTAGAAAACCTGTCCGACCTCTACTTCCTCCTGCGAGTCCCCCAGCTCCAGTT
  TCTCATCTTGAATCAGAATCGCCTTTCGTCATGCAAGGCAGCCCACACTCCCTCGGAGAACCCAAGCTTA
  GAACAGCTTTTCCTTACAGAGAATATGCTGCAGCTGGCCTGGGAGACCGGCCTCTGTTGGGATGTTTTTC
  AAGGCCTTTCCCGCCTCCAGATTCTTTACCTGAGTAATAACTACCTTAATTTCCTTCCACCTGGGATATT
  TAACGACCTGGTTGCATTACGGATGCTTAGTCTTAGTGCTAACAAGCTGACCGTGCTCTCTCCGGGCAGT
  TTACCTGCTAATTTAGAGATTCTCGACATATCTAGAAATCAGCTTTTCTCTCCTGACCCTGCTTTGTTTT
  CTTCGCTTCGTGTTTTGGACATAACTCATAACGAGTTCGTCTGCAACTGTGAACTTAGCACTTTTATCTC
  CTGGCTCAACCAAACCAACGTCACCCTGTTCGGCTCTCCTGCAGACGTGTATTGCATGTACCCTAACTCA
  CTGCTAGGGGGCTCCCTCTACAACATATCCACCGAAGACTGCGATGAAGAGGAAGCCATGCGGTCCCTAA
  AGTTTTCCCTTTTCATCCTGTGCACGGTCACTTTGACTCTATTCCTCGTCATCACCCTTGTAGTCATAAA
  GTTCCGGGGAATCTGTTTCCTGTGCTATAAGACCATCCAGAAGCTGGTGTTCAAGGACAAGGTCTGGAGT
  TTGGAACCTGGTGCATATAGATATGATGCCTACTTCTGCTTCAGCAGCAAAGACTTTGAATGGGCACAGA
  ATGCTTTGCTCAAACACCTGGATGCTCACTACAGTTCCCGAAACAGGCTCAGGCTATGCTTTGAAGAAAG
  AGACTTCATTCCAGGGGAAAACCATATCTCCAACATCCAGGCGGCTGTCTGGGGCAGCAGGAAGACGGTG
  TGTCTAGTGAGCAGACACTTCCTGAAGGATGGTTGGTGCCTGGAGGCCTTCAGGTATGCCCAGAGCCGGA
  GTCTGTCTGATCTCAAGAGCATTCTCATCGTGGTGGTGGTGGGATCGCTGTCCCAGTATCAGCTGATGAG
  ACATGAGACCATCAGAGGGTTTCTGCAAAAGCAACAGTACTTGAGGTGGCCTGAAGACCTCCAGGATGTT
  GGCTGGTTTCTCGATAAACTCTCCGGATGCATTCTAAAGGAAGAAAAAGGAAAGAAAAGAAGCAGTTCCA
  TCCAGTTGCGAACCATAGCAACCATTTCCTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_016928
• Insert Size: 2622 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_016928.2, NP_058624.2
• RefSeq Size: 3211 bp
• RefSeq ORF: 2622 bp
• Locus ID: 53791
• UniProt ID: Q9JLF7
• Cytogenetics: 1 86.12 cM
• Gene Summary: Pattern recognition receptor (PRR) located on the cell surface that participates in the activation of innate immunity and inflammatory response. Recognizes small molecular motifs named pathogen-associated molecular pattern (PAMPs) expressed by pathogens and microbe-associated molecular patterns (MAMPs) usually expressed by resident microbiota. Upon ligand binding such as bacterial flagellins, recruits intracellular adapter proteins MYD88 and TRIF leading to NF-kappa-B activation, cytokine secretion and induction of the inflammatory response. Plays thereby an important role in the relationship between the intestinal epithelium and enteric microbes and contributes to the gut microbiota composition throughout life.[UniProtKB/Swiss-Prot Function]