Pde5a (NM_153422) Mouse Untagged Clone

CAT#: MC222287

Pde5a (untagged) - Mouse phosphodiesterase 5A, cGMP-specific (Pde5a), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Pde5a
• Synonyms: Cgbpde; Cn5n; Pde5; Pde5a1; PDE5a2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC222287 representing NM_153422
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAACGAGCGGGCCCCAACTCCGTGCGGTCGCAGCAGCAGCGGGACCCGGACTGGGTGGAAGCGTGGC
  TGGATGATCACCGGGACTTTACCTTCTCTTACTTTATTAGAAAGGCCACCAGAGACATGGTCAACGCATG
  GTTTTCAGAGAGAGTTCACAACATCCCTGTGTGCAAGGAAGGCATCCGAGCCCATACCGAGTCCTGCTCT
  TGCTCTCTGCAGCAAAGTCCTCATGCAGATAATACCACCCCTGGAGCACCAGCCAGAAAAATATCTGCCT
  CTGAATTTGACCGACCTCTTAGACCCATTGTTGTCAAGGATTCCGAGGGAACAGTGAGCTTTCTCTCTGA
  CTCAGGAAAAAAGGAACAAATGCCACTAACCCCCCCAAGATTTGATAGTGATGAAGGGGACCAGTGCTCA
  AGGCTCTTGGAGCTAGTAAAAGATATTTCCAGTCATTTGGATGTCACAGCATTGTGTCACAAAATTTTCT
  TGCATATCCATGGACTCATCTCTGCGGATCGCTATTCCCTGTTCCTTGTCTGTGAGGACAGCTCTAAAGA
  CAAATTCCTCATCAGCCGCCTCTTTGATGTTGCTGAAGGTTCAACATTGGAGGAAGCTTCAAATAACTGT
  ATCCGTTTAGAGTGGAACAAAGGCATTGTGGGACATGTGGCAGCTTTTGGCGAGCCCTTGAACATCAAAG
  ATGCCTATGAGGATCCCCGGTTCAATGCAGAAGTTGACCAAATTACAGGCTACAAGACACAAAGTATCCT
  TTGTATGCCAATTAAGAACCACAGGGAAGAGGTTGTTGGTGTAGCTCAGGCCATCAACAAGAAATCAGGA
  AATGGTGGGACCTTCACTGAAAAAGATGAAAAGGACTTTGCGGCCTACCTGGCATTCTGTGGTATTGTTC
  TTCACAATGCTCAGCTCTATGAAACGTCATTGCTGGAGAACAAAAGAAATCAGGTGTTACTTGACCTTGC
  CAGCTTAATCTTTGAAGAGCAGCAGTCACTGGAAGTCATTCTGAAGAAGATAGCGGCCACTATCATCTCC
  TTCATGCAGGTGCAGAAGTGCACCATCTTCATTGTGGATGAAGACTGTCCTGACTCTTTCTCTCGTGTGT
  TTCACATGGAGTGTGAGGAAGTAGGAAAACCCTCTGATCCTTTAACGAGGGAGCAAGATGCAAACAAAAT
  CAACTACATGTATGCTCAGTATGTCAAAAACACGATGGAGCCACTTAATATCCCAGATGTCACCAAGGAC
  AAAAGATTTCCCTGGACAAATGAAAACATGGGACACGTGAACACGCCCTGCATTGGAAGTTTGCTTTGCA
  CCCCTATAAAAAATGGGAAGAAGAACAAAGTCATAGGCGTCTGCCAACTTGTTAATAAGATGGAGGAGAA
  TACTGGCAAGATTAAAGCTTTCAACCAAAATGATGAGCAGTTCCTGGAAGCCTTTGTCATCTTCTGTGGC
  TTGGGGATCCAGAACACACAGATGTATGAAGCGGTGGAGAGAGCCATGGCCAAGCAGATGGTGACATTAG
  AGGTCCTGTCTTACCATGCATCAGCGGCGGAGGAAGAAACAAGAGAGCTCCAGGCTTTATCGGCTGCTGT
  GGTACCATCGGCCCAAACCCTTAAAATTACCGACTTCAGCTTCAGTGACTTTGAGCTGTCTGATCTGGAA
  ACAGCGCTGTGTACAATTCGGATGTTCACTGACCTCAACCTTGTGCAGAACTTCCAGATGAAACATGAGG
  TTCTTTGCAGGTGGATTTTAAGTGTGAAGAAGAATTATCGGAAGAACGTTGCCTACCACAATTGGAGGCA
  TGCCTTTAACACGGCACAGTGCATGTTTGCTGCTCTGAAAGCCGGCAAGATCCAGAACAAGTTGACGGAT
  CTGGAGACCCTTGCGTTGCTCATTGCTGCTTTAAGCCATGACTTGGATCATCGTGGCGTGAACAACTCAT
  ACATACAGCGGAGCGAGCACCCGCTGGCTCAGCTGTACTGTCACTCCATCATGGAACACCATCATTTTGA
  CCAGTGCTTGATGATTCTAAACAGCCCAGGCAACCAGATTCTCAGTGGCCTCTCCATTGACGAATATAAG
  ACCACACTGAAAATAATCAAGCAAGCAATTTTAGCCACAGACCTAGCACTGTACATTAAGAGACGAGGAG
  AATTTTTTGAACTTATACGAAAAAATCAATTCAGTTTTGAAGATCCTCTTCAAAAGGAGTTGTTTCTAGC
  AATGCTGATGACAGCCTGCGACCTCTCTGCGATTACTAAACCCTGGCCTATTCAACAACGGATAGCAGAA
  CTCGTGGCAGCTGAATTCTTTGATCAAGGAGACAGAGAGAGAAAAGAACTTAACATGGAGCCGGCTGATC
  TAATGAACAGAGAGAAGAAAAATAAAATCCCAAGTATGCAAGTTGGGTTCATAGATGCCATCTGCTTGCA
  GCTGTATGAGGCCCTGACCCACGTATCCGAAGACTGTTTGCCCTTGCTGGATGGCTGCAGGAAGAACAGA
  CAGAAATGGCAGGCCCTCGCGGAGCAGCAGGAGAAGATGCTCCTTAACGGGGAGAGCAGCCAGGGCAAGC
  GGGACTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_153422
• Insert Size: 2598 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_153422.2, NP_700471.2
• RefSeq Size: 6728 bp
• RefSeq ORF: 2598 bp
• Locus ID: 242202
• UniProt ID: Q8CG03
• Cytogenetics: 3 G1
• Gene Summary: Plays a role in signal transduction by regulating the intracellular concentration of cyclic nucleotides. This phosphodiesterase catalyzes the specific hydrolysis of cGMP to 5'-GMP. Specifically regulates nitric-oxide-generated cGMP.[UniProtKB/Swiss-Prot Function]