Musk (NM_010944) Mouse Untagged Clone

CAT#: MC222258

Musk (untagged) - Mouse muscle, skeletal, receptor tyrosine kinase (Musk), transcript variant 4, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Musk
• Synonyms: Mdk4; Mlk; Ns; Nsk1; Nsk2; Nsk3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC222258 representing NM_010944
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGAGAGCTTGTCAACATTCCACTGTTACAGATGCTCACCCTGGTTGCCTTCAGCGGGACTGAGAAAC
  TTCCAAAAGCCCCTGTCATCACCACGCCTCTTGAAACTGTAGATGCCTTGGTTGAAGAAGTAGCGACTTT
  CATGTGTGCCGTGGAATCCTACCCTCAGCCCGAGATTTCTTGGACCAGAAATAAAATTCTCATTAAGCTG
  TTTGACACCCGCTACAGCATCCGGGAGAATGGTCAGCTCCTCACCATTCTGAGCGTGGAAGACAGTGATG
  ATGGCATCTACTGCTGCATAGCCAACAATGGAGTGGGAGGAGCCGTGGAGAGTTGTGGTGCCCTGCAAGT
  GAAGATGAAACCTAAAATAACTCGTCCTCCCATTAATGTAAAAATAATAGAGGGATTGAAGGCAGTTCTG
  CCGTGCACTACGATGGGTAACCCCAAACCATCTGTGTCCTGGATCAAGGGGGACAATGCTCTCAGGGAAA
  ATTCCAGAATCGCAGTTCTTGAATCTGGGAGCTTAAGGATCCATAATGTGCAAAAGGAAGATGCAGGACA
  GTACCGCTGTGTGGCCAAAAACAGCCTGGGCACAGCTTACTCCAAACTGGTGAAGCTGGAAGTGGAGGTT
  TTTGCAAGAATCCTGCGTGCTCCTGAATCCCACAATGTCACCTTTGGTTCCTTTGTAACCCTACGCTGCA
  CAGCAATAGGCATCCCTGTCCCCACCATCAGCTGGATTGAAAACGGAAATGCTGTTTCTTCAGGTTCCAT
  TCAAGAGAGTGTGAAAGACCGAGTGATTGACTCAAGACTCCAGCTCTTCATCACAAAGCCAGGACTCTAC
  ACATGCATAGCTACCAATAAGCACGGAGAAAAGTTCAGTACCGCAAAGGCTGCAGCCACTGTCAGCATAG
  CAGAATGGAGTAAGTCACAGAAAGACAGCCAAGGCTACTGTGCCCAGTACAGAGGGGAGGTGTGTGATGC
  AGTCCTGGCGAAAGATGCTCTTGTCTTCTTCAACACCTCCTACCGGGACCCCGAGGACGCCCAGGAGCTG
  CTGATCCACACTGCGTGGAATGAGCTGAAGGCTGTGAGTCCACTGTGCCGGCCAGCTGCTGAGGCTCTGC
  TGTGTAACCACCTCTTCCAAGAGTGCAGCCCTGGAGTGGTACCTACTCCCATGCCCATTTGCAGAGAGTA
  CTGCCTGGCGGTAAAGGAGCTCTTCTGTGCAAAGGAATGGCAGGCAATGGAAGGAAAGGCCCACCGGGGC
  CTCTACAGATCTGGGATGCATCTCCTTCCGGTACCAGAGTGCAGCAAGCTTCCCAGCATGCACCGGGACC
  CCACAGCCTGCACAAGACTGCCATATTTAGATTATAAAAAAGAAAACATAACAACATTCCCGTCAATAAC
  GTCCTCCAGGCCGAGCGCGGACATTCCAAACCTGCCTGCCTCCACCTCTTCCTTTGCCGTCTCGCCTGCG
  TACTCCATGACCGTCATCATCTCCATCGTGTCCAGCTTTGCCCTGTTTGCTCTTCTCACCATCGCTACTC
  TCTATTGCTGCCGAAGGAGGAAAGAATGGAAAAATAAGAAAAGAGAGTCGACCGCGGTGACCCTCACCAC
  GTTGCCTTCCGAGCTCCTGCTGGATAGGCTCCATCCCAACCCCATGTACCAGAGGATGCCACTCCTTCTG
  AATCCTAAGTTGCTCAGCCTGGAGTATCCGAGGAATAACATTGAGTATGTCCGAGACATCGGAGAGGGGG
  CGTTTGGAAGAGTCTTCCAAGCAAGGGCCCCTGGCTTGCTGCCTTATGAACCTTTCACTATGGTGGCCGT
  GAAGATGCTTAAGGAAGAGGCCTCTGCAGACATGCAAGCGGACTTTCAGAGGGAGGCGGCCCTCATGGCA
  GAGTTTGACAACCCCAACATTGTGAAACTCTTAGGTGTGTGTGCCGTTGGGAAGCCGATGTGTCTGCTCT
  TTGAATATATGGCCTATGGTGACCTCAATGAGTTCCTCCGAAGTATGTCCCCGCACACTGTTTGCAGCCT
  CAGCCACAGTGACCTGTCCACGAGGGCTCGGGTGTCTAGCCCTGGTCCTCCACCACTGTCCTGTGCAGAA
  CAGCTCTGCATTGCCAGGCAGGTGGCAGCTGGCATGGCCTACCTTTCAGAGCGCAAGTTTGTCCACCGGG
  ACTTAGCTACCAGGAACTGCCTGGTTGGGGAGACCATGGTGGTGAAAATTGCAGACTTTGGCCTCTCCAG
  GAACATCTATTCCGCAGACTACTACAAAGCTGATGGAAATGACGCCATCCCTATCCGCTGGATGCCGCCC
  GAGTCTATCTTCTACAACCGCTACACCACTGAGTCGGATGTATGGGCCTATGGTGTGGTCCTCTGGGAGA
  TCTTCTCCTATGGGCTGCAGCCCTACTATGGAATGGCCCACGAGGAGGTCATTTACTATGTGAGAGATGG
  CAACATCCTCGCCTGCCCTGAGAACTGCCCCTTGGAACTGTACAACCTCATGCGCCTGTGTTGGAGCAAG
  CTGCCTGCTGATAGACCCAGCTTCTGCAGTATCCACAGGATCCTGCAGCGCATGTGCGAGAGAGCAGAGG
  GAACGGTGGGTGTCTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_010944
• Insert Size: 2607 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_010944.2, NP_035074.2
• RefSeq Size: 3354 bp
• RefSeq ORF: 2607 bp
• Locus ID: 18198
• UniProt ID: Q61006
• Cytogenetics: 4 31.87 cM
• Gene Summary: This gene encodes a member of the protein tyrosine kinase family. The encoded protein is a type 1 receptor-like protein located in muscle membrane that is activated by the heparan sulfate proteoglycan agrin released by nerve cells. The encoded protein activates signaling cascades responsible for multiple aspects of motor neuron and muscle development, including organization of the postsynaptic membrane, synaptic gene transcription, patterning of skeletal muscle, anchoring of acetylcholinesterase, and guidance of motor axons. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (4) lacks two alternate, in-frame segments in the coding region, compared to variant 1. The encoded isoform (4) is shorter than isoform 1.