E2f8 (NM_001013368) Mouse Untagged Clone

CAT#: MC222175

E2f8 (untagged) - Mouse E2F transcription factor 8 (E2f8), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: E2f8
• Synonyms: 4432406C08Rik; AA410048
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC222175 representing NM_001013368
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGAACCAAAAGGAAAACCTCTTTTCTGAGCCACATAAAAGGGGACTGATGAAAAGCCCCCTGCATC
  CGTCCTCGAAGGCCAACATGGTGTTGGCTGAGATCCAGCCTGACTTGGGCCCTCTAACCACACCAACCAA
  GCCCAAGGAAGTCTCCCAAGGAGAGCCATGGACACCCACAGCCAACCTGAAAATGCTCATCAGCGCCGTG
  AGCCCCGAGATCCGAAGTCGAGATCAGAAAAGGGGCCTGTCTGACAACCGAAGTGCATTACCTGAAGCTA
  GAGACTGTTTGCACGAACACTTATCAGGAGACGAATTTGAGAAATCCCAGCCGAGTCGGAAGGAGAAGAG
  CTTGGGGTTGCTATGCCACAAATTCCTAGCGCGGTACCCCAAGTACCCCAACCCTGCTGTGAATAACGAC
  ATCTGCCTGGACGAGGTGGCCGAGGAGCTCAATGTTGAACGTCGGCGGATTTATGACATTGTGAATGTCC
  TAGAGAGCCTGCACATGGTGAGCCGCCTTGCCAAAAACAGGTACACTTGGCATGGCCGGCACAACCTCAC
  CAAAACCCTCGGGACGCTGAAGAGTGTCGGGGAAGAGAACAAGTACGCTGAGCAGATCATGATGATCAAA
  AGGAAAGAATATGAGCAAGAGTTTGATTTTATCAAGAGCTGTGGCATAGAGGACCACGTGATCAAGTCAC
  ACACTGGCCAGAATGGGCATTCAGACATGTGCTTCGTAGAACTCCCTGGAGTAGAATTCCGGGCAGCTTC
  TGTAAACAGCCGCAAAGACAAGTCCTTACGCGTGATGAGCCAGAAGTTTGTGATGCTGTTCTTGGTGTCG
  ACGCCTCAGATAGTGAGCCTGGAAATTGCTGCCAAGATTTTAATTGGGGAAGACCACGTGGAAGATCTGG
  ATAAAAGCAAGTATAAAACAAAAATTAGGAGGCTGTATGACATTGCTAATGTCCTGAGTAGCTTGGATCT
  TATCAAGAAAGTCCATGTTACAGAAGAAAGAGGTCGAAAACCAGCTTTTAAATGGACGGGCCCAGAAATC
  AGCCCAAACAACAGTGGTTCCAGCCCCATCATGCCTCTTCCTGCCTCCTTAGAGGCTGAGCAGTCTGCAA
  AAGAGAACTGTGCCAAAAACCTCTTCTCTACGCGGGGGAAACCCAGCTTCACTCGACACCCGTCCCTCAT
  CAAGTTGGTAAAGAGCATAGAAAATGATCGGAGGAAGATCAGTTCCGCTCCCAGCAGCCCTGTCAAGAGC
  AACAAAGCTGAGAGTTCTCAAAATTCTCCACCCGTCCCAAACAAAATGGCTCAGCTCGCCGCTATTTGCA
  AGATGCAGTTGGAAGAGCAGTCAAGTGAACCCAGGAAGAAAGTGAAAGTAAACCTAGCAAGATCTGGGCA
  CTACAAACCACTGGCTCCCCTGGACCCTACAGTGAACACTGAGCTAGAACTGCTTACACCGTCCCTTATC
  CAGCCCCTGGGCGTGGTCCCCCTGATCCCTAGCCCATTGTCATCTGCAGTGCCTGTGATCCTACCTCAGG
  CCCCTTCGGGCCCATCCTATGCCATCTACTTACAACCTGCCCAAGCCCAAATGTTGACACCACCCCCTGG
  CCTGAGCCCAACCGTCTGCCCCACCCAGCCCTCTAATGCTACTGGATCCAAAGACCCTACAGACGCCCCT
  GCTGAGAAGACGGCCACAGACGCCGCCACAACCGGAAGCTTGCAGCCAGCACCAGAAAGGCATGGTGCTA
  AGCATCGAAGCAAGGAGACGACCGGAGATCGGGGTACAAAGAGGATGATCACCGCGGAGGACAGTGGTCC
  CAGTTCTGTAAAGAAACCTAAAGAGGACCTGAAAGCGCTTGAGAACGTCCCCACCCCCACGCCCCTGTTC
  CCATCAGGATACCTAATCCCTCTTACCCAGTGCTCATCCCTAGGGCCAGACTCTGTGTTGTCTAACACTG
  AAAACTCAGGTACACCCTCTCCAAACCACCGGATCTATGGCTCCCCCATTGCAGGTGTGATCCCAGTGGC
  ATCATCGGAACTCACTGCTGTGAATTTTCCCCCATTCCATGTGACACCTCTGAAACTTATGGTCTCCCCA
  ACATCTATGGCGGCCGTACCTGTTGGGAACAGCCCAGCCCTCAACTCCGGCCACCCTGCTCCCGCCCAGA
  ACCCAAGCTCGGCTATCGTAAACTTTACCCTGCAGCACTTGGGACTCATCTCCCCGGGCGTGCAGATGTC
  TGCCAGCCCGGGGCCTGGAGCTGGCACAGTCCCGGTATCCCCACGTGTAGAAGCAGATAACTTGAGCTCT
  CGGCAAAGGAGGGCCACCAACCATGACTCGCCAGTCCTGGGCCAGAGCCAACTAAACGGACAACCAGTCG
  CGGGGACAGGGGCACAGCAGCCTGTTCCTGTGACACCCAAAGGCTCCCAGTTGGTGGCTGAAAATTTCTT
  CCGTACTCCGGGTGGACCAACTAAGCCTACCAGCTCACCCTACACAGATTTCGATGGTGCTAACAAAACC
  TCCTTCGGAACCCTCTTTGTCCCACAGCGCAAACTGGAAGTCTCAACTGAGGATATCCACTGAAGCGGAC
  CG
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-RsrII
• ACCN: NM_001013368
• Insert Size: 2592 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001013368.5, NP_001013386.2
• RefSeq Size: 3705 bp
• RefSeq ORF: 2583 bp
• Locus ID: 108961
• UniProt ID: Q58FA4
• Cytogenetics: 7 B4
• Gene Summary: Atypical E2F transcription factor that participates in various processes such as angiogenesis and polyploidization of specialized cells. Mainly acts as a transcription repressor that binds DNA independently of DP proteins and specifically recognizes the E2 recognition site 5'-TTTC[CG]CGC-3'. Directly represses transcription of classical E2F transcription factors such as E2F1: component of a feedback loop in S phase by repressing the expression of E2F1, thereby preventing p53/TP53-dependent apoptosis. Plays a key role in polyploidization of cells in placenta and liver by regulating the endocycle, probably by repressing genes promoting cytokinesis and antagonizing action of classical E2F proteins (E2F1, E2F2 and/or E2F3). Required for placental development by promoting polyploidization of trophoblast giant cells. Acts as a promoter of sprouting angiogenesis, possibly by acting as a transcription activator: associates with HIF1A, recognizes and binds the VEGFA promoter, which is different from canonical E2 recognition site, and activates expression of the VEGFA gene.[UniProtKB/Swiss-Prot Function]