Usp5 (NM_013700) Mouse Untagged Clone

CAT#: MC222154

Usp5 (untagged) - Mouse ubiquitin specific peptidase 5 (isopeptidase T) (Usp5), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Usp5
• Synonyms: AA407472; ISOT; ISOT-1; Ucht
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC222154 representing NM_013700
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGAGCTGAGTGAAGAGGCGCTGCTGTCAGTGTTACCGACGATCCGTGTCCCCAAGGCGGGAGACC
  GGGTCCATAAAGACGAGTGCGCTTTCTCTTTCGACACGCCGGAGTCTGAGGGTGGCCTCTATATCTGCAT
  GAACACATTCCTGGGATTCGGGAAGCAGTATGTGGAGAGACACTTCAACAAGACAGGCCAGCGTGTCTAC
  CTGCACCTCCGGAGGACCCGGCGACCGAAAGAAGAGGACACCAGTGCAGGCACTGGAGACCCACCTCGGA
  AGAAGCCCACCCGGCTGGCCATTGGTGTTGAAGGAGGGTTTGACCTCACCGAGGACAAGTTTGAATTTGA
  CGAGGATGTGAAGATTGTCATTTTGCCCGATTACCTGGAGATCGCTCGGGATGGGTTGGGGGGGCTTCCT
  GACATTGTCAGAGATCGGGTGACCAGTGCTGTGGAAGCCCTACTTTCTGCTGACTCAGCCTCTCGAAAGC
  AGGAGGTCCAGGCTTGGGATGGGGAAGTCCGTCAGGTGTCCAAGCATGCCTTCAACCTCAAGCAGTTGGA
  CAACCCTGCTCGGATCCCTCCCTGTGGCTGGAAGTGCTCCAAGTGCGACATGCGCGAGAACCTGTGGCTC
  AACCTGACAGACGGCTCCATCCTCTGTGGACGGCGCTACTTCGATGGCAGTGGGGGCAACAACCATGCTG
  TGGAGCACTACAGGGAGACTGGCTACCCCTTAGCTGTTAAGCTGGGCACCATCACCCCGGATGGAGCTGA
  CGTGTATTCTTACGACGAGGATGACATGGTTCTGGACCCCAGCCTGGCAGAGCACTTGTCTCACTTTGGC
  ATCGACATGCTGAAGATGCAGAAGACGGACAAGACGATGACTGAGCTGGAGATAGACATGAACCAGCGGA
  TTGGTGAATGGGAGTTGATCCAGGAGTCAGGCGTGCCACTCAAGCCTCTGTTTGGGCCTGGTTACACGGG
  CATCCGGAACCTGGGTAACAGCTGCTATCTCAACTCCGTGGTCCAGGTGCTCTTCAGCATCCCTGACTTT
  CAGAGGAAGTATGTGGATAAATTGGAGAAGATCTTCCAGAATGCCCCAACGGACCCCACCCAAGACTTCA
  GCACCCAGGTGGCCAAGCTGGGGCATGGCCTTCTCTCTGGAGAATACTCTAAGCCAGCACTGGAGTCAGG
  TGATGGGGAGCAGGTACCAGAACAAAAGGAAGTTCAAGATGGCATTGCCCCTCGAATGTTCAAGGCCCTC
  ATTGGCAAGGGCCACCCTGAGTTCTCCACTAATCGGCAGCAGGATGCCCAGGAATTCTTCCTACATCTTA
  TCAACATGGTGGAGAGGAATTGCCGGAGTTCTGAAAATCCAAATGAAGTGTTCCGCTTTTTGGTGGAAGA
  AAAGATCAAATGCCTGGCCACAGAGAAGGTTAAGTACACTCAGCGAGTTGACTACATCATGCAGTTGCCT
  GTACCCATGGATGCAGCCCTTAACAAAGAGGAGCTTTTAGAGTATGAAGAGAAGAAGCGGCAAGCTGAAG
  AGGAGAAAGTGCCACTGCCAGAACTGGTTCGGGCCCAGGTGCCCTTCAGCTCCTGCCTGGAAGCCTACGG
  GGCCCCTGAGCAGGTGGACGACTTCTGGAGTACAGCTCTACAGGCGAAGTCGGTCGCTGTCAAGACCACG
  CGCTTTGCCTCCTTCCCCGACTACCTGGTCATCCAGATCAAGAAGTTCACCTTTGGCTTAGACTGGGTGC
  CCAAGAAGCTGGATGTGTCCATTGAGATGCCAGAGGAGCTCGATATCTCCCAGCTGAGGGGCACAGGGCT
  ACAGCCGGGAGAGGAGGAGCTGCCTGACATTGCCCCACCCCTGGTCACTCCGGATGAGCCCAAAGGTAGC
  CTTGGTTTCTATGGCAACGAAGACGAAGACTCCTTCTGCTCCCCTCACTTCTCCTCTCCGACATCACCCA
  TGCTGGACGAATCCGTCATCATACAGTTGGTGGAAATGGGCTTCCCTATGGATGCCTGCCGGAAAGCTGT
  GTACTATACGGGAAACAGCGGGGCCGAAGCAGCCATGAACTGGGTCATGTCACACATGGATGATCCAGAT
  TTTGCAAACCCCCTCATCCTGCCTGGCTCCAGTGGGCCTGGCTCCACAAGTGCAGCAGCTGACCCCCCGC
  CGGAGGACTGTGTGACCACCATAGTCTCCATGGGCTTCTCCAGGGACCAGGCCCTGAAAGCTCTGCGGGC
  CACGAACAATAGCTTAGAGCGGGCTGTAGACTGGATCTTCAGCCACATTGATGACCTGGACGCAGAAGCT
  GCTATGGACATCTCAGAGGGACGCTCAGCGGCTGAGTCCATCTCCGAGTCTGTGCCAGTGGGACCTAAAG
  TCCGGGATGGTCCTGGAAAGTATCAGCTCTTTGCCTTCATTAGTCACATGGGCACATCTACTATGTGTGG
  TCACTATGTCTGCCATATCAAGAAGGAAGGCAGGTGGGTGATCTACAATGACCAGAAAGTGTGTGCCTCT
  GAAAAGCCACCCAAGGACCTGGGCTACATCTACTTCTACCAGAGAGTGGTCAGCTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_013700
• Insert Size: 2577 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_013700.2, NP_038728.1
• RefSeq Size: 3233 bp
• RefSeq ORF: 2577 bp
• Locus ID: 22225
• UniProt ID: P56399
• Cytogenetics: 6 59.17 cM
• Gene Summary: Cleaves linear and branched multiubiquitin polymers with a marked preference for branched polymers. Involved in unanchored 'Lys-48'-linked polyubiquitin disassembly. Binds linear and 'Lys-63'-linked polyubiquitin with a lower affinity (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).