St14 (NM_011176) Mouse Untagged Clone

CAT#: MC222151

St14 (untagged) - Mouse suppression of tumorigenicity 14 (colon carcinoma) (St14), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: St14
• Synonyms: Epithin; matriptase; mCAP3; MT-SP1; Prss14; Tmprss14
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC222151 representing NM_011176
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGTAGCAATCGGGGCCGCAAGGCCGGAGGGGGCTCTCAGGACTTCGGCGCGGGACTCAAGTACAACT
  CCCGGCTAGAGAACATGAATGGCTTTGAGGAGGGTGTGGAGTTCCTGCCTGCGAACAATGCCAAGAAAGT
  GGAGAAGCGAGGCCCCAGGCGCTGGGTGGTGCTGGTGGCAGTGCTGTTCAGCTTCCTCTTGCTCTCCCTC
  ATGGCTGGCTTGCTGGTGTGGCACTTCCATTATCGGAATGTGCGGGTTCAAAAAGTCTTCAATGGCCATC
  TGAGGATCACAAATGAGATCTTTCTGGATGCGTATGAGAACTCCACCTCCACAGAGTTTATCAGCCTGGC
  CAGCCAGGTGAAGGAGGCGCTGAAGCTGCTGTACAATGAAGTCCCTGTCCTGGGTCCCTACCACAAGAAG
  TCGGCTGTAACTGCCTTCAGTGAGGGCAGTGTCATCGCCTACTACTGGTCAGAGTTCAGCATCCCCCCAC
  ACCTGGCAGAAGAGGTTGATCGCGCCATGGCTGTGGAGCGAGTTGTAACATTGCCACCCCGAGCACGGGC
  ACTGAAATCCTTCGTGCTAACATCTGTGGTGGCCTTCCCCATTGACCCCAGAATGCTGCAGAGGACTCAG
  GACAACAGCTGCAGTTTTGCCCTGCATGCCCATGGTGCAGCAGTGACACGCTTCACTACCCCTGGCTTCC
  CCAACAGTCCCTACCCGGCGCATGCCCGCTGCCAGTGGGTCCTGCGGGGGGACGCCGACTCTGTGCTGAG
  CCTCACCTTCCGAAGCTTTGATGTCGCTCCCTGTGATGAGCATGGCAGTGACCTGGTCACCGTGTATGAT
  AGCCTGAGCCCCATGGAACCCCACGCTGTGGTGCGGCTGTGTGGCACCTTCTCACCCTCCTACAACCTGA
  CTTTCCTCTCCTCCCAGAACGTCTTCCTTGTCACGCTGATAACCAATACTGACCGGCGACATCCTGGCTT
  TGAGGCCACTTTCTTCCAGCTGCCCAAGATGAGCAGCTGTGGCGGCTTTTTGAGTGACACCCAAGGGACA
  TTTAGCAGCCCCTACTATCCAGGCCACTACCCGCCCAACATCAACTGCACATGGAATATCAAGGTGCCCA
  ACAACCGGAACGTGAAGGTGCGCTTCAAACTCTTCTATCTGGTGGACCCCAACGTACCAGTGGGCTCCTG
  CACCAAGGACTATGTGGAGATCAACGGGGAGAAGTACTGCGGTGAGAGGTCCCAGTTTGTGGTGAGCAGC
  AACAGCAGCAAGATTACAGTCCACTTCCATTCTGATCACTCGTACACGGACACCGGGTTCCTAGCTGAGT
  ACCTCTCCTACGACTCCAACGACCCGTGCCCAGGGATGTTCATGTGCAAGACTGGACGGTGCATCCGAAA
  GGAACTGCGCTGCGACGGCTGGGCAGACTGCCCGGATTATAGTGATGAGCGTTACTGCCGATGCAATGCC
  ACCCACCAGTTCACGTGCAAAAACCAGTTCTGCAAGCCCCTCTTCTGGGTCTGTGACAGTGTCAACGACT
  GTGGGGACGGAAGTGACGAGGAGGGCTGCAGCTGTCCTGCTGGGAGTTTCAAGTGTTCCAATGGGAAGTG
  TCTCCCTCAGAGCCAGAAGTGTAATGGGAAGGACAACTGTGGAGATGGGTCTGACGAGGCTTCATGTGAC
  AGCGTGAATGTCGTCTCTTGCACCAAATATACCTACCGCTGCCAAAATGGCCTCTGTCTGAGCAAGGGCA
  ACCCTGAGTGTGATGGGAAGACGGACTGTAGCGATGGCTCCGATGAGAAAAACTGTGACTGTGGGCTGCG
  ATCCTTTACCAAACAGGCTCGCGTGGTTGGTGGCACGAATGCGGACGAGGGCGAGTGGCCCTGGCAGGTG
  AGCCTCCACGCCCTGGGCCAGGGCCACTTGTGTGGGGCCTCGCTCATCTCTCCTGACTGGCTGGTCTCTG
  CAGCTCATTGCTTTCAGGATGACAAAAATTTCAAGTACTCAGACTACACGATGTGGACGGCCTTCCTGGG
  TCTGCTGGACCAGAGCAAGCGCAGTGCCTCTGGGGTGCAGGAGCTGAAGCTCAAACGTATCATCACCCAC
  CCTTCCTTCAATGATTTCACCTTCGACTATGACATCGCCTTGCTGGAGCTGGAGAAGTCGGTGGAGTACA
  GCACCGTCGTGCGCCCCATCTGCCTGCCTGATGCTACCCATGTCTTCCCTGCTGGCAAGGCCATCTGGGT
  CACAGGCTGGGGGCACACAAAAGAGGGAGGTACCGGAGCGCTGATCCTGCAGAAGGGTGAGATCCGTGTC
  ATCAACCAGACCACCTGTGAGGACCTCATGCCGCAGCAGATCACCCCACGAATGATGTGTGTGGGTTTCC
  TCAGTGGGGGTGTGGACTCCTGCCAGGGTGACTCTGGTGGCCCCTTGTCAAGCGCGGAGAAAGATGGGCG
  AATGTTCCAGGCTGGTGTGGTGAGCTGGGGTGAAGGCTGCGCTCAGAGGAACAAGCCAGGCGTGTACACA
  AGGCTCCCTGTAGTTCGGGACTGGATCAAAGAGCACACTGGGGTATAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_011176
• Insert Size: 2568 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_011176.4, NP_035306.2
• RefSeq Size: 4017 bp
• RefSeq ORF: 2568 bp
• Locus ID: 19143
• UniProt ID: P56677
• Cytogenetics: 9 16.61 cM
• Gene Summary: Degrades extracellular matrix. Exhibits trypsin-like activity as defined by cleavage of synthetic substrates with Arg or Lys as the P1 site (By similarity). Involved in the terminal differentiation of keratinocytes through prostasin (PRSS8) activation and filaggrin (FLG) processing (By similarity).[UniProtKB/Swiss-Prot Function]