Ddx21 (NM_019553) Mouse Untagged Clone

CAT#: MC222055

Ddx21 (untagged) - Mouse DEAD (Asp-Glu-Ala-Asp) box polypeptide 21 (Ddx21), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Ddx21
• Synonyms: AI255159; AL022742; D10Ertd645e; D10Wsu42e
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC222055 representing NM_019553
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCGGGAAAACTCCGCAGTGGGGCCAAGTTGGGCTCAGACGGGGCCGAGGAGAGCATGGAGACGCTGC
  CGAAGCCAAGCGAGAAGAAAACTAGAAAAGAGAAGACAAAATCAAAGACCGAAGAAGCAACAGAAGGAAT
  GGAAGAGGCCGTTTCCTCCAAAGCAAAGAAAACAAACAAGAAAGGGCCTTCTGAGGATGATGTGGATCCC
  CCTAAATCCAGGAAGGCCAAGAAGCAGGAGGAGGAGCCTCAAGACGACACTGCTTCTACAAGCAAAACCT
  CCAAGAAGAAAAAGGAGCCCCTTGAGAAGCAAGCAGACTCTGAAACCAAAGAGATAATAACAGAGGAGCC
  TTCCGAAGAAGAGGCAGACATGCCTAAGCCCAAGAAGATGAAGAAAGGGAAAGAAGCAAACGGAGATGCC
  GGAGAGAAAAGCCCCAAGCTGAAGAATGGACTCTCCCAACCTTCCGAAGAAGAGGCAGACATTCCTAAGC
  CCAAGAAGATGAAGAAAGGGAAAGAAGCAAACGGAGATGCTGGAGAGAAAAGCCCCAAGCTGAAGAATGG
  ACTCTCCCAACCTTCCGAAGAAGAGGTAGACATTCCTAAGCCCAAGAAGATGAAGAAAGGGAAAGAAGCA
  AGCGGAGATGCGGGAGAGAAAAGCCCCAGGCTGAAGGATGGACTCTCCCAACCTTCTGAACCGAAATCCA
  ACTCCAGTGATGCTCCTGGTGAGGAAAGTAGTAGCGAGACAGAAAAGGAAATACCTGTGGAGCAGAAAGA
  GGGAGCTTTCTCTAATTTTCCCATTTCTGAAGAGACTGTCAAGCTTCTCAAAGCTCGTGGGGTGAACTTC
  CTGTTTCCTATACAAGCCAAGACCTTTCATCATGTGTACAGTGGGAAAGACTTAATTGCCCAGGCACGGA
  CAGGAACCGGAAAGACATTCTCTTTTGCCATCCCTTTAATTGAGAAACTTCAAGGTGGGCTACAAGAGAG
  GAAGAGAGGCCGAGCCCCTCAGGTGCTAGTCCTTGCACCCACAAGAGAATTAGCAAATCAAGTGAGCAAA
  GACTTCAGCGACATCACCAAAAAGCTCTCTGTAGCTTGTTTTTATGGTGGAACACCCTACGGTGGTCAGA
  TTGAACGAATGCGGAGTGGGATTGATATCCTGGTTGGGACCCCAGGTCGTATTAAAGACCACTTGCAGAA
  TGGCAAGCTAGATCTCACCAAACTTAAACATGTTGTCCTGGATGAAGTCGACCAGATGTTGGATATGGGT
  TTTGCTGATCAAGTGGAAGAAATTTTATGTGTTGCATACAAGAAAGATTCTGAAGACAACCCCCAAACAT
  TGCTTTTCTCTGCAACTTGCCCTCATTGGGTGTTTAATGTTGCTAAGAAATACATGAAATCTACATACGA
  ACAGGTGGACCTGATTGGTAAAAAGACTCAGAAAGCAGCTATAACTGTGGAGCACCTGGCGATTAAGTGT
  CACTGGACAGAAAGGGCAGCGGTGATTGGGGATGTGATCCGAGTGTACAGTGGTCATCAAGGGCGCACGA
  TCATCTTCTGTGAAACCAAGAAGGATGCCCAGGAGCTATCTCAGAACACGTGCATAAAGCAGGATGCCCA
  GTCCTTACATGGCGACATTCCACAGAAGCAAAGGGAAATCACCCTGAAAGGTTTCCGAAATGGCAATTTT
  GGAGTTTTGGTGGCAACCAATGTTGCTGCCCGTGGGTTAGACATCCCTGAGGTTGACTTGGTTGTTCAGA
  GCTGCCCACCAAAGGATGTGGAGTCTTACATTCATCGTTCGGGGCGGACAGGCAGAGCTGGAAGGACAGG
  AGTTTGCATCTGCTTTTACCAGAACAAAGAAGAGTACCAGTTAGCACAAGTGGAGCAGAAAGCGGGAATT
  AAATTTAAACGGATAGGTGTTCCTTCTGCAACGGAAATAATAAAAGCCTCCAGCAAAGACGCCATCAGGC
  TCTTGGATTCTGTGCCTCCCACTGCCATTAGCCATTTCAAGCAGTCAGCTGAGAAGCTGATTGAGGAGAA
  GGGAGCTGTAGAGGCCTTGGCAGCTGCTCTGGCCCACATCTCAGGAGCCACATCAGTCGATCAGCGCTCC
  CTGATCAACTCGCAAGCAGGCTTTGTGACCATGATCCTGCGGTGCTCCATTGAGATGCCCAACATCAGTT
  ACGCTTGGAAAGAACTTAAGGAGCAGCTGGGCGAGAGCATCGATGCCAAAGTGAAGGGAATGGTCTTCCT
  CAAAGGAAAACTGGGGGTTTGTTTTGATGTCCGAACTGAAGCAGTCACAGAAATACAGGAGAAGTGGCAT
  GATTCGAGACGCTGGCAGCTCACCGTGGCCACCGAGCAGCCAGAGCTGGAGGGACCTCCAGACGGATATC
  GAGGCCGCATGGGTCAGCGTGATGGCAGCCGTGGTGCTTTCAGAGGACAGCGGGGTGGAAGCAGGAACTT
  CCGGGGACAGGGACAGCGAGGAGGAAGTAGAAACTTCCGAGGACAGCGACCAGGAGGTGGCAACAGAGGC
  CAGAAGCGGAGTTTTAGTAAAGCGTTTGGTCAGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_019553
• Insert Size: 2556 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_019553.2, NP_062426.2
• RefSeq Size: 4763 bp
• RefSeq ORF: 2556 bp
• Locus ID: 56200
• UniProt ID: Q9JIK5
• Cytogenetics: 10 32.43 cM
• Gene Summary: RNA helicase that acts as a sensor of the transcriptional status of both RNA polymerase (Pol) I and II: promotes ribosomal RNA (rRNA) processing and transcription from polymerase II (Pol II). Binds various RNAs, such as rRNAs, snoRNAs, 7SK and, at lower extent, mRNAs. In the nucleolus, localizes to rDNA locus, where it directly binds rRNAs and snoRNAs, and promotes rRNA transcription, processing and modification. Required for rRNA 2'-O-methylation, possibly by promoting the recruitment of late-acting snoRNAs SNORD56 and SNORD58 with pre-ribosomal complexes. In the nucleoplasm, binds 7SK RNA and is recruited to the promoters of Pol II-transcribed genes: acts by facilitating the release of P-TEFb from inhibitory 7SK snRNP in a manner that is dependent on its helicase activity, thereby promoting transcription of its target genes. Functions as cofactor for JUN-activated transcription: required for phosphorylation of JUN at 'Ser-77'. Can unwind double-stranded RNA (helicase) and can fold or introduce a secondary structure to a single-stranded RNA (foldase). Involved in rRNA processing. May bind to specific miRNA hairpins (By similarity). Component of a multi-helicase-TICAM1 complex that acts as a cytoplasmic sensor of viral double-stranded RNA (dsRNA) and plays a role in the activation of a cascade of antiviral responses including the induction of proinflammatory cytokines via the adapter molecule TICAM1 (PubMed:21703541).[UniProtKB/Swiss-Prot Function]