Sema4g (NM_011976) Mouse Untagged Clone

CAT#: MC222003

Sema4g (untagged) - Mouse sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4G (Sema4g), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Sema4g
• Synonyms: AI507908; AW554132
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC222003 representing NM_011976
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTGGGGGAGGCTCTGGCCCCTCCTCTTCAGCTTTCTCACAGTAACAGCAGTGCCTGGACCCTCACTGC
  GGAGACCATCCCGAGAGCTAGATGCCACCCCACGGCTGACCATCTCCTATGAAGAGCTCTCCCAGATTCG
  GCACTTCAAGGGCCAAACCCAGAACTATTCAACACTGCTGCTGGAGGAGGCTTCTGAGAGGCTGCTGGTG
  GGGGCTCGAGGCGCCCTGTTCTCTCTCAGTGCCCGTGACATAAGAGATAGGACTCACAAAGAGATCCACT
  GGGAGGCCTCCCCAGAGATGCAAAGCAAGTGTCATCAGAAAGGGAAAAACAACCAGACCGAGTGTTTCAA
  CCACGTGCGATTTCTACAGCGGCTCAATGCCACCCACTTCTATGCATGTGGGACCCACGCCTTCCAGCCC
  CTCTGTGCAGCTATTGATGCTGAAACCTTCATCTTGCCTACTAGTTTCGAGGAGGGAAAGGAGAAATGTC
  CTTATGACCCGGCCCGGGGCTTCACAGGCCTTATCATTGATGGAGGCCTCTACACAGCCACCCGGTATGA
  ATTCCGGAGCATTCCTGATATCCGCCGGAGCCGCCACCCACACTCTCTGAGAACTGAGGAGGCACCCATG
  CACTGGCTCAATGATGCTGAGTTTGTGTTCTCTGTGCTGGTGCGGGAGAGCAAGACAAGCGCAGTGGGTG
  ATGATGACAAGATTTATTTCTTCTTCATGGAGCGTGAGGAAGGCTCCAGCAGCTTCACACAGAGTCGCAG
  CAGCCACCGTGTGGCTAGAGTAGCCCGTGTGTGCAAGGGAGATCTGGGAGGGAAGAAGATCCTACAGAAG
  AAGTGGACATCCTTCCTGAAGGCTCGTCTCATCTGCCACATCCCACAGTATGAGACACTACGTGGGGTCT
  GCAGCCTGAATGCTGATACATCATCCCACACACATTTCTATGCCGTCTTCACACTGACAACACAGTGGAA
  GACGCTGGAGGCTTCAGCCATCTGCCGCTATGACCTGGCTGAGATTCAGGCTGTCTTCACAGGCCCTTTT
  ATGGAGTACCAGGATGGTGCCCGGCGCTGGGGCCGCTATGAGGGTGGGGTGCCTGAACCCCGGCCTGGCT
  CGTGCATCACAGATTCATTGCGCAGCCGAGGCTACAACTCATCCCAAGACTTGCCATCCCTGGTTCTGGA
  CTTTGTGAAGTTGCATCCCCTGATGGCTCGGCCTGTGGTGCCCACACGTGGGCGGCCCTTGCTGCTGAAG
  CGCAATGTACGCTACACACATCTCACAGGAACACACGTCAGCACACCTGCTGGACCCACCTATGACCTGC
  TCTTTCTGGGCACAGCTGATGGCTGGATCCACAAGGCCGTAGTCCTGGGCTCTGGGATGCACATAATTGA
  AGAGATACAAGTGTTCAGGGAGCCCCAGTCTGTGGACAATCTAGTTATCTCACCAATGCAGCACAGCCTC
  TACGTGGGGGCTGCAAGTGGAGTCCTCCAGTTTCCTCTGTCCAGCTGCTCACGCTACCAGTCCTGCTATG
  ACTGCATCCTGGCACGGGACCCCTACTGTGGCTGGGACTCCAGCATCCATGCCTGCATGGTGGCCACCAC
  CGTAGCCAACAGGACAGAATTGATACAGGACATAGAGAGAGGAAATCGAGGCTGTGAGGGCAGCAGGGAT
  GCAGGACCACCACCACCACTCAAGACCCGATCTGTGCTCCGGGGTGATGATGTCCTCCTGCCCTGTGACC
  AGCCGTCTAACCTGGCCCGGGCTTTGTGGCTACTCAATGGGAGCAAGAGCCTGAGCGATGGGCAGGATGG
  CTACCGCGTGGGAGTGGATGGGCTGCTGGTAACTGACACACAACTGGAGCACAGTGGCAACTATGGCTGC
  TATGCCGAGGAGAATGGCCTCCGTATGCTGCTGGCGTCCTACAGCCTCACAGTCCGGCCAGCCACCCCTG
  CTCCAGCTCCACAAGCCCCTGCCACACCTGGGGCACAGCTGGCACATGACATGAGGATGTTCTATGTGGT
  AGCCATTGCTATACTTGGTGGCCTCTGCCTCATCTTGGCATCTTCCCTCCTCTATGTGGCTTGTCTGAAG
  GGAGGCAGACGAGGTCGAAGACGAAAATACTCATTGGGTCGGGCTGGCCGGGCAGGGGGCTCGGCCGTGC
  AGCTGCAGACTGTCTCAGGCCAGTGTCCTGGAGAGGAAGATGAAGGTGATGATGGAGAAGGAACTGGGGG
  TCTGGAGAGTGGCTGCCTCCAGATCATTCCCGGGGAAGGAGCCCCAGCCCCACCACCCCCACCACCCCCA
  CCCCCACCCGCTGAGCTGACCAATGGGCTGGTGGCACTGCCCAGCCGGCTGCGAAGGATGAATGGCAACA
  GTTATGTGCTCCTGAGGCAGAGCAACAATGGAGTGCCAGCAGGACCCTGCTCCTTTGCAGAGGAGCTCAG
  CCGCATCCTGGAGAAGAGGAAGCACACACAGCTTGTAGAGCAACTAGACGAGAGCTCTGTCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_011976
• Insert Size: 2514 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_011976.1, NP_036106.1
• RefSeq Size: 4310 bp
• RefSeq ORF: 2514 bp
• Locus ID: 26456
• UniProt ID: Q9WUH7
• Cytogenetics: 19 C3
• Gene Summary: Cell surface receptor for PLXNB2. May play a role in axon guidance.[UniProtKB/Swiss-Prot Function]