Zc3hav1 (NM_028864) Mouse Untagged Clone

CAT#: MC221645

Zc3hav1 (untagged) - Mouse zinc finger CCCH type, antiviral 1 (Zc3hav1), transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Zc3hav1
• Synonyms: 1200014N16Rik; 2900058M19Rik; 9130009D18Rik; 9830115L13Rik; ARTD13; D6Bwg1452e; ZAP
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC221645 representing NM_028864
  Red=Cloning site Blue=ORF
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACGGATCCCGAGGTATTCTGTTTCATCACCAAGATCCTGTGCGCTCACGGGGGCCGCATGACCCTGG
  AGGAACTGCTGGGTGAGATCAGCCTCCCCGAAGCGCAACTCTACGAGCTGCTGAAGGCAGCAGGGCCCGA
  TCGCTTTGTGCTATTGGAGACTGGAGACCAGGCCGGGATCACTCGGTCGGTGGTGGCTACTACTCGAGCC
  CGCGTCTGCCGTCGCAAGTACTGCCAGAGACCCTGCGACAGCCTGCACCTTTGCAAGCTTAATCTGCTCG
  GCCGGTGCCACTATGCACAGTCCCAGCGGAACCTCTGCAAATATTCTCACGATGTTCTCTCGGAACAGAA
  CTTCCAGGTCCTGAAGAATCATGAGCTCTCCGGGCTTAACCAAGAGGAGCTGGCGGTCCTCCTGGTCCAA
  AGCGACCCTTTCTTCATGCCTGAGATATGCAAGAGTTACAAAGGAGAGGGCCGCAAACAGATCTGCGGGC
  AGCCGCAGCCCTGCGAGAGACTCCACATCTGTGAGCACTTCACCCGGGGCAACTGCAGTTACCTCAACTG
  TCTCAGGTCTCATAACCTGATGGACAGGAAGGTGTTGGCCATCATGAGGGAGCATGGGCTGAGTTCTGAT
  GTGGTCCAGAACATCCAGGATATCTGCAACAACAAACACACTCGGAGGAACCCCCCTAGCATGAGAGCTC
  CCCACCCACATCGCAGAGGCGGGGCACACAGGGACAGAAGCAAAAGCAGAGACCGCTTCCATCACAACAG
  TCTAGAGGTTCTCTCAACGGTCTCACCTCTGGGATCTGGTCCCCCTAGCCCAGATGTCACCGGCTGTAAG
  GATCCCCTGGAGGATGTGTCTGCAGATGTCACCCAGAAGTTCAAGTACCTGGGGACTCAGGACCGTGCAC
  AGCTTTCCTCCGTCTCATCTAAGGCCGCTGGTGTCCGAGGACCCAGTCAAATGAGAGCAAGCCAGGAGTT
  TTTGGAGGATGGGGATCCAGATGGCTTGTTTTCTAGGAATCGTTCTGATTCGTCCACAAGTCGAACCTCT
  GCTGCTGGCTTTCCTCTCGTTGCGGCACAAAGAAATGAAGCTGGGGCCATGAAAATGGGCATGCCTTCAG
  GACACCACGTCGAGGTCAAGGGCAAGAACGAGGACATTGATCGCGTCCCGTTTTTAAATAGTTATATTGA
  TGGGGTAACAATGGAAGAAGCAACAGTCTCAGGAATTCTAGGTAAAAGGGCCACAGACAACGGTCTGGAA
  GAAATGATACTATCTAGCAACCATCAGAAGAGTGTGGCTAAGACCCAGGATCCCCAGACCGCTGGCAGAA
  TCACTGACAGTGGCCAAGACACGGCATTCCTGCATAGTAAATATGAAGAAAACCCAGCGTGGCCAGGTAC
  ATCTACCCATAACGGCCCAAATGGCTTTAGTCAAATTATGGATGAAACGCCTAATGTCTCTAAAAGTAGT
  CCCACTGGTTTTGGCATAAAATCAGCAGTCACTGGAGGAAAAGAAGCAGTCTATTCTGGAGTTCAGAGTC
  TGAGAAGCCATGTCCTGGCTATGCCTGGGGAGACCACTACTCCTGTACAGGGCAGCAATAGGCTGCCTCC
  GTCACCTCTGTCTTCTTCCACAAGCCACAGAGTTGCAGCCTCTGGGAGCCCTGGCAAGAGCTCCACCCAT
  GCCTCTGTGAGCCCAGCCAGTGAGCCCTCGAGGATGATGATGATGATGTCAGACCCTGCTGAGTATTCCC
  TATGCTACATCGTAAATCCTGTATCTCCTAGGATGGATGATCATGGCCTGAAGGAAATCTGTCTGGATCA
  TCTGTACAGGGGCTGTCAGCAGGTCAACTGCAACAAGAACCACTTCCATCTGCCCTACCGGTGGCAGCTG
  TTCATATTGCCCACTTGGATGGACTTTCAGGACATGGAGTATATCGAGCGGGCCTATTGTGATCCCCAAA
  TTGAAATCATTGTGATAGAAAAACATCGGATCAATTTCAAGAAAATGACTTGTGATTCCTACCCCATCCG
  TCGCCTCTCCACTCCTTCATTTGTCGAAAAAACACTTAATTCTGTCTTCACCACCAAGTGGCTTTGGTAT
  TGGAGGAATGAATTGAATGAATATACTCAGTATGGGCATGAGAGCCCAAGCCATACCAGCTCCGAAATTA
  ATTCTGCATACCTGGAGTCTTTCTTCCACTCCTGTCCCAGGGGAGTTTTGCAGTTCCACGCTGGTTCACA
  GAATTACGAGTTAAGCTTTCAAGGGATGATTCAGACGAATATAGCTTCCAAGACTCAAAGGCATGTTGTG
  AGAAGGCCAGTTTTTGTTTCTTCGAAGGATGTGGAGCAGAAGAGAAGAGGTCCAGAGTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_028864
• Insert Size: 2370 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_028864.2, NP_083140.1
• RefSeq Size: 3396 bp
• RefSeq ORF: 2370 bp
• Locus ID: 78781
• UniProt ID: Q3UPF5
• Cytogenetics: 6 17.72 cM
• Gene Summary: Antiviral protein which inhibits the replication of viruses by recruiting the cellular RNA degradation machineries to degrade the viral mRNAs. Binds to a ZAP-responsive element (ZRE) present in the target viral mRNA, recruits cellular poly(A)-specific ribonuclease PARN to remove the poly(A) tail, and the 3'-5' exoribonuclease complex exosome to degrade the RNA body from the 3'-end. It also recruits the decapping complex DCP1-DCP2 through RNA helicase p72 (DDX17) to remove the cap structure of the viral mRNA to initiate its degradation from the 5'-end. Its target viruses belong to families which include retroviridae: human immunodeficiency virus type 1 (HIV-1) and moloney and murine leukemia virus (MoMLV), filoviridae: ebola virus (EBOV) and marburg virus (MARV), togaviridae: sindbis virus (SINV) and Ross river virus (RRV). Specifically targets the multiply spliced but not unspliced or singly spliced HIV-1 mRNAs for degradation. Isoform 1 is a more potent viral inhibitor than isoform 2. Isoform 2 acts as a positive regulator of DDX58/RIG-I signaling resulting in activation of the downstream effector IRF3 leading to the expression of type I IFNs and IFN stimulated genes (ISGs).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) lacks several exons and its 3' terminal exon extends past a splice site that is used in variant 3. This results in a novel 3' coding region and 3' UTR, compared to variant 3. It encodes isoform 2 which is shorter and has a distinct C-terminus, compared to isoform 3.