Smpd4 (NM_001164611) Mouse Untagged Clone

CAT#: MC221644

Smpd4 (untagged) - Mouse sphingomyelin phosphodiesterase 4 (Smpd4), transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Smpd4
• Synonyms: 4122402O22Rik; mKIAA1418
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC221644 representing NM_001164611
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGTTCCCTCACCTACAACAGCCTAGTTTCCTTCTGGCTAGCCTGAAAGCTGACTCTATAAATAAGC
  CCTTTGCACAACGTTGCCAAGATTTGGTGAAAGTCATTGAGGATTTTCCAGCCAAGGAATTGCATGCCGT
  CTTCCCATGGCTGGTGGAAAGCATCTTTGGCAGCCTGGATGGTGTCCTTGTTGGTTGGAATCTCCGATGC
  CTACAGGGACGTGTGAACCCTGTGGAGTACAGCACAGCGATGGAATTTCTAGACCCAAGTGGTCCAATGA
  TGAAGTTGGTTTATAAACTTCAAGCTGAAGACTATAACTTTGATTTTCCTGTCTCCTGTCTTCCTGGCCC
  TGTGAAGGCATCCATTCAAGAGAATGTCCTCCCTGACAGTCCTCTGTACCACAACAAAGTCCAGTTTCCC
  CCGACCGGAGGCCTTGGCCTGAACCTGGCCCTCAATCCATTTGAATACTATATGTTCTACTTTGCTTTGA
  GCCTCATCTCTCAAAAGCCAATGTCTATGACCCTCCATGTCCGTACTTCGGACTGTGCCTATTTCACCCT
  GGTGGATAGGTACCTATCATGGTTCCTACCCACTGAAGGCAGCGTACCTCCACCACTCTGCTCCAGTCCA
  GGAGGCTCCAGCCCCTCACCAGCTCCCAGAACACCAGCCATGCCCTTTGCCTCCTATGGCCTCCACACTA
  GCCTCTTGAAGCGACACATCTCTCATCAGACATCTGTGAATGCAGACCCTGCATCCCATGAGATCTGGCG
  GTCAGAAACTCTCCTCCAGGTTTTTGTGGAAATGTGGCTTCATCATTACTCCCTGGAGATGTACCAAAAA
  ATGCAGTCCCCTCATGCCAAGGAGTCCTTCACGCCTACTGAAGAGCACGTGCTGGTGGTACGCCTGCTGC
  TGAAGCACCTGCATGCCTTTGCCAACAGCCTGAAGCCAGATCAGGCTTCGCCATCTGCACACTCCCATGC
  CACCAGTCCGCTGGAGGAATTCAAACGGGCCGCAGTGCCTCGCTTTGTCCAGCAGAAGCTGTACGTCTTC
  CTGCAGCACTGTTTTGGCCACTGGCCTCTGGATGCAACCTTCAGAGCTGTCTTGGAGATGTGGCTGAGCT
  ACCTGCAGCCATGGAGGTATGCCCCTGAGAAACAGGCTCAGGGCAGCGATCCCCAGCCACGGTGTGTGTC
  AGAGAAGGCACCCTTCATCCAGGAAAACCTGCTGATGTACACCAAGCTGTTTGTGAGCTTTCTGAACCGT
  GCCCTACGCACAGACCTTGTCAGCCCCAAGAATGCACTCATGGTCTTCCGAGTGGCTAAGGTCTTTGCCC
  AGCCCAACCTGGCTGAGATGATCCAGAAAGGTGAGCAGCTGTTCCTGGAGCCGGAGCTCATCATCCCCCA
  CCGCCAACACCGGCTCTTCACAGTCACCACCAGCTTCCTGTCACCATGGCCCCCTGTTGTGACAGATGCC
  TCCTTCAAGGTGAAGAGCCATGTCTATAGCCTAGAGGGCCAAGACTGCAAATATACCCCAATGTTTGGTC
  CTGAAATCCGAACCCTGGTCTTGCGCCTTGCTCAGCTCATCACACAGGCCAAGCAGACTGCCAAGTCCAT
  CTCTGATCAGTATGTGGAAAGCCCAACTGGCCGCTCCTTCCTGTCATGGCTGACCTTTGGCCTCACAGAC
  ACAAATAGCTGCTACCCAGCCAATGACCTGGATGAAATAGGTCAGGACAGCATCCGCAAGACAGACGAGT
  ACTTAGAAAAGGCCCTGGAGTACCTGCGCCAGATATTCCGGCTCAGCGAAGCCCAGCTGGCCCAGCTCAC
  GCTTGCCCTGGGGAGTGCTCGGGACGAGAATGGGAAGCAGCAGCTCCCAGATTGCATTGTGGGGGAAGAA
  GGACTCATCCTTACACCCTTGGGCCGCTACCAGATCATTAATGGTCTGCGAAGGTTCGAGATTGAGTACC
  AGGGAGACTTAGAGCTGCAGCCCATCCGGAGCTACGAAATCACCAGTCTGGTCCGTGCACTCTTCCGGCT
  GTCCTCTGCCATCAACCGTAGATTTGCAGGCCAGATGGCAGCTCTGTGCTCCCGGAATGACTTCCTTGGC
  AGCTTCTGTCGCTACCACCTCACTGAGCCTGCCTTGAGCAACAGACATCTGCTGAGCCCTGTAGGGCGGA
  GGCAGGTCACCAATCCTGCACGGGGCCCCAGGCTCAGCCTCCGTTTCCTGGGCAGTTACCGGACACTGCT
  CCTGCTCCTGATGGCCTTCTTTGTGGCTTCTCTGTTCTGCATTGGGCCCCTGTCCTGCTCCTTGCTCCTG
  GTCCTAGGATACGTCCTCTATGCCATAGCTATGACACTGCTCACTGAACGGGGGAAGCTGCACCAGCTCT
  GA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001164611
• Insert Size: 2382 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001164611.1, NP_001158083.1
• RefSeq Size: 4542 bp
• RefSeq ORF: 2382 bp
• Locus ID: 77626
• UniProt ID: Q6ZPR5
• Cytogenetics: 16 A3
• Gene Summary: Catalyzes the hydrolysis of membrane sphingomyelin to form phosphorylcholine and ceramide. May sensitize cells to DNA damage-induced apoptosis.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) has multiple differences in the coding region but maintains the reading frame, compared to variant 1. This variant encodes isoform 2, which is shorter than isoform 1.