Cdh15 (NM_007662) Mouse Untagged Clone

CAT#: MC221555

Cdh15 (untagged) - Mouse cadherin 15 (Cdh15), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Cdh15
• Synonyms: AI323380; Cdh14; Mca; Mcad
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC221555 representing NM_007662
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGTTCTGCTCTGCTCCTCGCCCTCGGGCTGCTTGCCCAGAGCCTTGGCCTGTCCTGGGCAGTCCCTG
  AGCCCAAACCCAGCACCCTGTACCCCTGGCGCCGGGCATCAGCCCCAGGCCGTGTGCGGAGAGCCTGGGT
  CATCCCACCCATTAGTGTGTCTGAGAACCACAAACGCCTCCCCTACCCACTTGTGCAGATCAAGTCTGAC
  AAACAGCAGCTAGGCAGTGTCATCTACAGCATCCAGGGTCCCGGAGTGGATGAGGAGCCCCGAAATGTCT
  TCTCCATCGACAAGTTCACCGGGAGGGTGTACCTCAATGCCACGCTGGACCGCGAGAAGACGGACCGCTT
  CAGGCTAAGGGCCTTTGCCTTGGACTTGGGTGGCTCTACCCTGGAGGACCCCACGGACCTGGAGATCGTT
  GTGGTGGATCAAAATGACAACCGGCCAGCCTTCCTACAGGATGTGTTCAGAGGCCGCATCCTGGAGGGTG
  CCATCCCAGGCACCTTCGTCACCAGGGCTGAGGCCACAGATGCCGACGACCCAGAGACAGACAATGCAGC
  CCTCAGGTTCTCCATCCTGGAGCAGGGCAGCCCTGAGTTCTTCAGCATCGACGAGCACACGGGAGAGATC
  CGCACGGTGCAAGTGGGGCTGGACCGTGAGGTGGTGGCTGTGTATAATCTGACCTTGCAAGTGGCAGACA
  TGTCGGGAGACGGACTCACTGCCACAGCCTCGGCCATCATCTCCATAGATGACATCAACGACAATGCCCC
  CGAGTTCACCAAGGATGAGTTCTTTATGGAGGCTGCAGAGGCTGTCAGTGGAGTGGACGTGGGACGGCTC
  GAGGTGGAAGACAAGGACCTGCCTGGTTCCCCCAACTGGGTGGCCAGGTTCACCATCCTTGAAGGTGATC
  CTGATGGGCAGTTCAAGATCTACACAGACCCTAAGACCAATGAAGGTGTGCTGTCCGTGGTCAAGCCCCT
  GGACTATGAGAGCCGTGAGCAGTATGAGCTCAGAGTGTCTGTACAAAACGAGGCCCCGCTGCAGGCAGCT
  GCCCCTCGGGCTCGGCGGGGCCAGACCAGGGTCAGCGTGTGGGTTCAGGACACCAACGAAGCTCCAGTGT
  TTCCAGAGAACCCACTGAGGACGAGCATAGCTGAAGGAGCCCCCCCAGGCACCTCTGTGGCCACCTTCTC
  TGCCAGAGACCCTGACACAGAACAGCTGCAGAGAATCAGCTACTCCAAAGACTACGACCCAGAAGACTGG
  CTGCAAGTGGACGGGGCCACAGGCAGGATCCAGACCCAGCGAGTGCTGAGCCCTGCTTCACCCTTTTTGA
  AGGACGGCTGGTACAGAGCCATCATCCTAGCCCTGGACAATGCCATTCCTCCTAGCACAGCCACAGGCAC
  CCTGTCCATCGAGATCCTAGAAGTCAACGACCATGCCCCTGCACTGGCTCTTCCTCCATCTGGCAGCCTG
  TGCAGTGAACCAGACCAGGGCCCTGGCCTCCTCTTGGGTGCCACGGATGAGGACCTGCCCCCACACGGGG
  CCCCTTTCCACTTCCAGCTGAACCCCAGAGTACCAGATCTGGGCCGGAACTGGAGCGTCAGCCAGATTAA
  CGTGAGCCATGCACGCTTGCGGCTCCGACATCAGGTCTCCGAGGGCCTGCATCGCCTGAGCCTGCTACTC
  CAGGACTCTGGGGAGCCACCCCAGCAGCGAGAGCAAACGCTGAACGTCACCGTGTGTCGCTGTGGGTCGG
  ATGGCACTTGCCTGCCCGGGGCTGCCGCGCTTCGAGGAGGAGGTGTAGGCGTCAGCTTGGGGGCACTGGT
  CATTGTGCTGGCCAGCACCGTGGTCTTACTAGTTCTCATCCTGCTTGCCGCGCTCCGCACACGGTTCCGG
  GGGCATTCCCGGGGCAAGAGTCTGTTGCATGGCCTACAAGAGGACCTTCGGGACAACATCCTTAACTATG
  ATGAGCAAGGAGGCGGGGAGGAGGACCAGGATGCGTACGACATAAACCAGCTGCGCCACCCAGTGGAGCC
  GAGGGCCACAAGTCGCTCTTTGGGCAGGCCACCCCTGCGCAGGGATGCCCCCTTCAGCTATGTGCCACAG
  CCACATCGAGTGCTTCCCACCAGCCCATCTGACATTGCCAACTTCATCAGTGATGGCTTGGAGGCTGCGG
  ACAGCGACCCCAGCGTGCCTCCCTACGACACAGCTCTCATCTATGACTACGAGGGAGATGGCTCTGTGGC
  AGGGACGCTGAGCTCCATTCTGTCCAGCCTGGGAGATGAAGACCAGGACTATGACTACCTCCGGGACTGG
  GGACCCCGCTTTGCTCGGCTGGCGGACATGTATGGACATCAGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_007662
• Insert Size: 2355 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_007662.2, NP_031688.2
• RefSeq Size: 2843 bp
• RefSeq ORF: 2355 bp
• Locus ID: 12555
• UniProt ID: P33146
• Cytogenetics: 8 71.99 cM
• Gene Summary: This gene encodes a member of the cadherin family of calcium-dependent glycoproteins that mediate cell adhesion and regulate many morphogenetic events during development. The encoded preproprotein is further processed to generate a mature protein. Based on the expression of this gene in skeletal muscle, satellite cells and cerebellum, it was postulated that the encoded protein may be important for muscle development and regeneration. Mice lacking the encoded protein appear normal and display no discernible defects in skeletal musculature. Multiple distinct genes of the cadherin family, including this gene, are found on chromosome 8. [provided by RefSeq, Nov 2015]