Cul1 (NM_012042) Mouse Untagged Clone

CAT#: MC221493

Cul1 (untagged) - Mouse cullin 1 (Cul1), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Cul1
• Synonyms: Cul-1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC221493 representing NM_012042
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCATCAAACAGGAGTCAGAATCCTCATGGACTGAAGCAGATTGGTCTTGACCAGATCTGGGATGACC
  TCAGAGCTGGAATTCAGCAGGTGTATACCAGACAAAGCATGGCAAAGTCCAGATACATGGAACTCTACAC
  TCATGTTTATAACTACTGTACTAGTGTGCATCAGTCCAACCAAGCCCGGGGTGCTGGAGTCCCTCCTTCC
  AAGTCAAAAAAGGGGCAGACTCCTGGGGGAGCTCAGTTTGTTGGCTTGGAGTTATACAAGCGATTGAAGG
  AATTTTTGAAGAATTATTTGACAAATCTTCTTAAGGATGGAGAAGATTTAATGGATGAGAGTGTCCTGAA
  GTTCTACACTCAGCAGTGGGAAGATTACCGATTCTCCAGCAAAGTGCTGAATGGAATCTGTGCCTACCTC
  AATAGACATTGGGTTCGCCGTGAATGTGATGAAGGACGAAAAGGAATATATGAAATCTATTCACTTGCAT
  TGGTGACATGGAGAGATTGTTTGTTCAGGCCATTGAATAAACAGGTAACAAATGCTGTTTTAAAGTTGAT
  TGAAAAGGAAAGAAATGGAGAAACCATCAATACAAGACTGATTAGTGGAGTTGTACAATCTTATGTGGAA
  TTGGGGCTGAATGAAGATGATGCATTTGCAAAGGGCCCTACGTTAACAGTGTATAAAGAATCCTTTGAAT
  CTCAATTTTTGGCTGACACAGAGAGATTTTATACCAGAGAAAGTACTGAATTCTTACAGCAGAACCCAGT
  TACTGAATATATGAAAAAGGCTGAAGCTCGTTTGCTTGAGGAACAGCGGAGAGTTCAGGTTTACCTCCAC
  GAGAGCACACAAGATGAACTAGCACGGAAATGTGAGCAGGTCCTCATTGAAAAACACTTGGAAATTTTTC
  ACACAGAGTTTCAGAATTTATTGGATGCTGACAAGAATGAAGATTTGGGCCGCATGTATAATCTTGTATC
  TAGAATTCAGGATGGCCTAGGAGAATTGAAAAAACTACTAGAGACACATATTCATAATCAGGGTCTTGCA
  GCAATTGAGAAGTGTGGAGAAGCTGCTCTAAATGACCCCAAGATGTATGTGCAGACAGTGCTGGATGTTC
  ATAAAAAATACAATGCCCTGGTGATGTCAGCATTCAACAACGATGCTGGCTTCGTAGCTGCTCTTGATAA
  GGCTTGTGGTCGCTTCATAAACAACAATGCGGTTACCAAAATGGCCCAGTCATCCAGTAAATCCCCTGAG
  CTGCTTGCTCGGTACTGTGACTCCTTGCTGAAGAAAAGTTCCAAGAACCCAGAAGAGGCAGAACTAGAAG
  ACACCCTCAATCAAGTGATGGTGGTCTTCAAGTACATCGAGGACAAAGATGTATTTCAGAAGTTCTATGC
  AAAGATGCTTGCCAAGAGACTGGTACATCAAAACAGTGCGAGTGACGATGCTGAAGCAAGCATGATCTCC
  AAGTTAAAGCAAGCTTGTGGTTTTGAGTACACTTCCAAACTTCAGCGAATGTTTCAAGACATTGGTGTAA
  GCAAAGATCTGAATGAACAGTTCAAAAAACACCTGACGAATTCAGAACCGCTGGACTTGGACTTCAGTAT
  TCAGGTCCTGAGTTCTGGATCGTGGCCCTTTCAGCAGTCTTGCACGTTTGCCTTGCCGTCAGAGTTGGAG
  CGCAGTTATCAGCGATTTACAGCTTTCTACGCCAGCCGTCACAGTGGCAGAAAATTGACATGGTTGTATC
  AACTGTCCAAAGGGGAACTAGTCACTAACTGCTTCAAAAACAGATACACTTTGCAGGCGTCCACATTTCA
  GATGGCAATCCTGCTTCAGTATAACACAGAAGACGCCTATACTGTGCAGCAGTTGACTGACAGCACTCAG
  ATTAAAATGGACATTTTGGCACAAGTCCTACAGATTTTACTGAAGTCGAAATTGCTGGTCTTAGAAGATG
  AAAATGCCAATGTTGATGAGGTGGAATTGAAGCCAGACACCTTAATAAAGTTATATCTTGGTTATAAAAA
  TAAGAAATTGAGGGTTAATATCAATGTACCAATGAAAACTGAACAGAAGCAGGAACAAGAAACTACACAC
  AAAAATATAGAGGAAGACCGCAAACTACTGATTCAGGCGGCCATCGTGAGAATCATGAAAATGAGGAAGG
  TCCTGAAACACCAGCAGTTACTTGGTGAAGTCCTCACTCAGCTCTCCTCCAGGTTCAAACCTCGGGTCCC
  GGTGATCAAGAAATGCATTGATATTCTCATCGAAAAAGAATATTTGGAGCGAGTCGATGGTGAGAAGGAC
  ACCTACAGTTACTTGGCTTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_012042
• Insert Size: 2331 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_012042.3, NP_036172.1
• RefSeq Size: 3171 bp
• RefSeq ORF: 2331 bp
• Locus ID: 26965
• UniProt ID: Q9WTX6
• Cytogenetics: 6 B2.3
• Gene Summary: Core component of multiple cullin-RING-based SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complexes, which mediate the ubiquitination of proteins involved in cell cycle progression, signal transduction and transcription. SCF complexes and ARIH1 collaborate in tandem to mediate ubiquitination of target proteins. In the SCF complex, serves as a rigid scaffold that organizes the SKP1-F-box protein and RBX1 subunits. May contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. The E3 ubiquitin-protein ligase activity of the complex is dependent on the neddylation of the cullin subunit and exchange of the substrate recognition component is mediated by TIP120A/CAND1. The functional specificity of the SCF complex depends on the F-box protein as substrate recognition component. SCF(BTRC) and SCF(FBXW11) direct ubiquitination of CTNNB1 and participate in Wnt signaling. SCF(FBXW11) directs ubiquitination of phosphorylated NFKBIA. SCF(BTRC) directs ubiquitination of NFKBIB, NFKBIE, ATF4, SMAD3, SMAD4, CDC25A, FBXO5 and probably NFKB2. SCF(BTRC) and/or SCF(FBXW11) direct ubiquitination of CEP68. SCF(SKP2) directs ubiquitination of phosphorylated CDKN1B/p27kip and is involved in regulation of G1/S transition. SCF(SKP2) directs ubiquitination of ORC1, CDT1, RBL2, ELF4, CDKN1A, RAG2, FOXO1A, and probably MYC and TAL1. SCF(FBXW7) directs ubiquitination of cyclin E, NOTCH1 released notch intracellular domain (NICD), and probably PSEN1. SCF(FBXW2) directs ubiquitination of GCM1. SCF(FBXO32) directs ubiquitination of MYOD1. SCF(FBXO7) directs ubiquitination of BIRC2 and DLGAP5. SCF(FBXO33) directs ubiquitination of YBX1. SCF(FBXO1) directs ubiquitination of BCL6 and DTL but does not seem to direct ubiquitination of TP53. SCF(BTRC) mediates the ubiquitination of NFKBIA at 'Lys-21' and 'Lys-22'; the degradation frees the associated NFKB1-RELA dimer to translocate into the nucleus and to activate transcription. SCF(CCNF) directs ubiquitination of CCP110. SCF(FBXL3) and SCF(FBXL21) direct ubiquitination of CRY1 and CRY2. SCF(FBXO9) directs ubiquitination of TTI1 and TELO2. SCF(FBXO10) directs ubiquitination of BCL2.[UniProtKB/Swiss-Prot Function]