Gcfc2 (NM_177884) Mouse Untagged Clone

CAT#: MC221471

Gcfc2 (untagged) - Mouse expressed sequence AW146020 (AW146020), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Gcfc2
• Synonyms: A130099G21; AW146020; GCF2; Tcf9
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC221471 representing NM_177884
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTCTCAGGCCCCAGAGGACTTTCAGGCGGCGCCAGGTCGAGTCCAGCGATAGCGATAGCGATAGCG
  ACGGCGCGAAGGAGCAGAGTGCGGAGGAGCCGGCGTCTGCCGGCGGCCGAACAGAGGGAGCGGAGCGGCC
  GCGCGGGGCCCGCAGCGCCCGGGGCCGAGGTCGGGTCTGGGCGAGTTCCCGCCGCAGCCCGGGAGCTGCT
  CCCCGCGGGGACGGCGGAGCAGAATGCAGAACAGCTGAGCTCTCAACGGATGAAGAGGAAGGAACACATA
  CCTTGACAGGAAGTAAGGGTGATCGGAGTCCGTCCTCTGATAGCTCTTGCTCTCTGGAAGAAAGAGACGT
  TTCACCCATAGTTGAGATCCCCGATGCAGCTTTTATTCAGGCAGCCCGCAGAAAACGGGAGTTGGCCAGG
  ACCCCAGGGGACTACATTTCATTGGATGTAAACCACTCCTGCTCCACTTCTGACTGTAAGAGAAGCAATG
  AAGAGGACCCAGAGAGCGATCCTGATGACCACGAAAAGAGAATACTGTTTACCCCAAAGCCTCAAACACT
  CCGGCAAAGAATGGCTGAAGAAACGTCAATCAGGAGTGAGGAATCAAGTGAAGAGAGTCAGGAAGATGAA
  AACCAAGACATCTGGGAGCAGCAGCAGATGAGGAAAGCAGTCAGGATCCCGGCGGGACAAAATACAGACC
  TTTCCCACAGCAGTAAGTCTCAAACCCTGAAGAAGTTTGATACTTCCATTTCATTTCCACCGGTAAATTT
  GGAAATTATAAAGAAACAGTTAAATAATAGATTAACATTACTACAGGAGTCTCATCGCTCACACCAGAGA
  GAATATGAAAAATATGAACAAGACATCAAGAGTTCAAAGACGGCTATCCAGAATCTTGAGAGTGCATCAG
  ACCATGCTCAAAACTACAGATTCTACAGGGGCATGAAAAGTTATGTGGAAAATATAATTGATTGTCTAAA
  TGAAAAGATTGTCAGCATTGTGGAACTAGAATCATCCATGTACACGTTGCTTTTAAAACGGTCAGAAGCA
  CTTTTGAAACGCAGGCAAGATGAATTAAAATGCGAATCATCATATTTGCAGCAGTTATCACGCAAAGATG
  AGACATCGGCAAATGGAAGCTTGGCTGTGGATGAAAAGGATCAACGGATTTTAGAAGAGATTGAAGCTCG
  GAGGATGCAGAGAAGACAGGCCAGGGAGCTCTCGGGCAGCTGTGACCATCAGGAAGGGATGTCTAGTGAT
  GATGAGCTGTCTCCTGCAGAGATGACTAACTTCCACAAGTGCCAAGGTGACATTTTACAGGATTGTAAGA
  AAGTTTTTGAAGATGTGCATGATGATTTTTGTAACGTCCAGAATATTTTATTGAAATTTCAGCAGTGGCG
  AGAAAAGTTTCCTGATTCCTACTATGAAGCCTTCGTTGGTTTCTGCTTACCAAAGCTTTTAAGTCCCCTG
  ATACGAGTTCAGTTGCTTGACTGGAATCCTCTTAAGATGGACTCCATAGGTCTAGACAAGATGCCCTGGT
  TCACAGCTATAACAGAGTTTATGGAGAGCAGCATGGACGATATAGGGAAGGAAGATGGTTCTGATAAAAA
  AATCTTGGCTGCTGTCATCAACAAAACAGTTGTTCCTCGACTTACAGATTTTGTAGAGACCATTTGGGAT
  CCCCTGTCAACCTCACAGACAAGAAGTTTAACAGTGCACTGCAGAGTGGCTTTTGAACAGTTCGCTTCTG
  AAAATGAAGTTAGTAAAAATAAACAGGATTTACTTAAATCTATTGTTGCAAGAATGAAGAAGTCAATAGA
  AGATGATATTTTTATTCCTCTATACCCAAAGAGCTCTGAAGAAGGAAAAATGTCACCACACTCCAAGTTC
  CAAGAAAGACAGTTCTGGGGAGCTCTAAAGCTCTTCCGAAATATTCTTCTTTGGAATGGCCTTCTCCCAG
  ATGACACCTTGCAAGATCTGGGACTGGGGAAGCTGCTGAATCGCTACCTTATCATCTCTCTTACTAATGC
  CGTCCCTGGACCAGATGTGGTTAAAAAGTGCAGCCAGATAGCAGCATGTCTACCAGAAAGATGGTTTGAG
  AACTCTGCTATGAGGACATCAATTCCCCAGCTAGAAAACTTCATTAAGTTTTTGTTGCAGTCTGCACAGA
  AATTATCCTCAAGTGAATTCAGGAATGAAGTCAGTGAAATAATTCTTATCCTGGTGAAGGTAAAAGCTCT
  GACTCAAGCCGAATCCCTCAGAGAAGAGCGTCCCCTGGAGCCCCTCCCAGCCCAGAGTACAGGTGTTTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_177884
• Insert Size: 2310 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_177884.3, NP_808552.2
• RefSeq Size: 3375 bp
• RefSeq ORF: 2310 bp
• Locus ID: 330361
• UniProt ID: Q8BKT3
• Cytogenetics: 6 C3
• Gene Summary: Factor that represses transcription. It binds to the GC-rich sequences (5'-GCGGGGC-3') present in the epidermal growth factor receptor, beta-actin, and calcium-dependent protease promoters. Involved in pre-mRNA splicing through regulating spliceosome C complex formation. May play a role during late-stage splicing events and turnover of excised inrons (By similarity).[UniProtKB/Swiss-Prot Function]