Terb1 (NM_180958) Mouse Untagged Clone

CAT#: MC221468

Ccdc79 (untagged) - Mouse coiled-coil domain containing 79 (Ccdc79), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Terb1
• Synonyms: 4930532D21Rik; BB085179; Ccdc79
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC221468 representing NM_180958
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAAAGTGAGAAGCCAAAGAAAACACAAGAAATGAAGACTGACCTGAAGTTACTGTTGGAATGTCTGA
  AGTATCACATGGGCAACCCTTTGTCACAAAAGGAAGTTTTGATCACGATTCATTCAGTTTGTAAACAAAA
  CAGTGATGCAGGTATTTATTTCCGGGAAATTGGTGGTTTGATGTTTATAATAAACCTTGCAAAGTCAAGT
  GAGCAGAGCTTGGTAAAAGAAGCAGCCTTATACACATTAGGATCTATTGCAGAAGAAAATGTTTACTGTC
  AGCAATCTTTGTGTACTTCAGAATTATTTCAAGATTTAACCGGGCTCTTAACTAATGATGATTCAAACAC
  AAATTTGAAAAGAATGTCTGTCTATGTTCTTTTGGTTCTGGTTTCAAATAATAGAAATGGACAAACTCTT
  GTGAGAGAAGTGGGTTGTATTGAAGTTCTGTCACAGATGTTCAGGACAGTTCTTTCCAATTATGAGTTGA
  ATTTGTCAGATAATAGTGTTTTCCAGAGCTATCTGCTATGGTCGTCTGTGTGCAGTACTCTGTGTGTATG
  TGTCAACAATCCTCAAAATGATGAGAATCAAATGCTTTGTTGTTCACTTTTTCCATGTGTTAATGAATGG
  CTAATGAACTGCATGAGACCTGAGGTAATTCGACCTATATGCTCATTTATTGGACTTACTCTTGCAAATA
  ATACACACGCTCAGAACTGCTTTGTCTCTTCGGGTGGACTGGATGTTTTGTGTCAAGTTCTTGTGCAGCT
  GGAGTCTGATTCACACAATACTCTTTCCAGTGCTAAGCTTGCAGTCATAGTGACAAAGACCATGGATGCA
  TGTATTACTGATAACTCAGCTGCTTTTACAGTGGTATTATCCAAGTACCACATTGTTTCTACACTTCTGG
  CATTACTGCTTCATGAAAGTCTTGATTCAAGAGAAAAATTTAGCATCATACTTGCCATTGGTCACTGCAC
  AGAGGATTGTGAGAAAAATCAGTATGAACTTTTAAAAAACAATGGGCTTCCATTGATGATACAAGCCTTA
  ACTGAGTTTAAGAATGAAGACCTAAGCAAAGCTGCCACTTACGTGCTTCACAACTGCAAAAAAATTACTG
  GGAAATTATCTCTAAGTCTAGGACAGAATTCTTTTGGTGAAAATGAAATTGAACTAAAGGACATAAGCGA
  AAAAGAGACTCTTAGAGAGCACTGGAAGGCAGCAAAGGAAATTCTATGCAGAATAAAACAATTTGAAAAA
  GGAGGGAAAGAGGAGAAGCAACAAAACAGGAGTGGACACTATAAGGATAATACCCCATCTATGAAAGTAA
  ACATACAGACCAATTTGAAACGTCTCTGTGCAGATAGTACTGGAGGTACCAGAGCAGAAGATAAGGATAT
  AAACCAGTCTAGAGAGCTTCGCAGCTATAAGCCCAGTGAAATCATGTCTAAAGCTTGTGCAAATGAAAAC
  CAACTGACGACTCGGAAGAAGAACACAAATCCAGTTCATCCTTTCTGTAAGGAGAAAGGGCAGAGTAAGA
  TTGTACATGAAACCACTCCAAGCTGTGCTCAAAACTTAGACAAGGAAAAAACCTTTGACCAAAAGGATTC
  TGTTTCTCAATCGAGTGACCAGGTCCTTAAACATCTGCCTCATACTGTCAAAAATAGAAAGCAGGTACCA
  GAAACAGATCCATTTACATTGTGTTTAGACATAATAGATAGAGAAGTTGGTATTCAAGCAACTGACAGCT
  GTTCCAGAATGTTAAAATATACGTGCTCAGGTTGCATAGTAGCAAGAAAACTCCTCAACAGCCGAAATTT
  TAGCAAGTTTTTGCACTCATGTGCATACCAGTGTGTTCACCATAAAGTCATTATGGAAGCTGAAGACAAA
  TATAAAAATGAACTAAGGAAAACATTTATCTGTGCAAAAAAAATTTTGCTGACTCCCTGTAGAAGAAGAC
  AACTTTGTAAAGAATCTACTGCCTCTGAAGAACTAAAAATTGTTCATCAAAAACCAGATAGTAAAAAACT
  TCCAGGTTTGGAGGCACAGGCCTTAAATACCAGCATTCCAGAGGCTATGGAAAGAAGATCACCAGTTCCA
  GGCCAGAGTGGACTACATAAAAAAAGAAGAATTCGTAAAGACTTTACCAAAGAAGAAGTAAATTATCTTT
  TCCATGGAGTTAAAACAATGGGAAATCATTGGAATTCAATTTTGTGGTCTTTCCCCTTTCAGAAAGGACG
  AAGGGCTGTAGACCTTGCTCACAAATACCACAGGCTGATCAAAGGCCCCAGCTGTGCAGCCCTGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_180958
• Insert Size: 2307 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_180958.3, NP_851289.2
• RefSeq Size: 2950 bp
• RefSeq ORF: 2307 bp
• Locus ID: 320022
• UniProt ID: Q8C0V1
• Cytogenetics: 8 D3
• Gene Summary: Meiosis-specific telomere-associated protein involved in meiotic telomere attachment to the nucleus inner membrane, a crucial step for homologous pairing and synapsis (PubMed:24885367, PubMed:24413433, PubMed:26548954). Component of the MAJIN-TERB1-TERB2 complex, which promotes telomere cap exchange by mediating attachment of telomeric DNA to the inner nuclear membrane and replacement of the protective cap of telomeric chromosomes: in early meiosis, the MAJIN-TERB1-TERB2 complex associates with telomeric DNA and the shelterin/telosome complex. During prophase, the complex matures and promotes release of the shelterin/telosome complex from telomeric DNA (PubMed:26548954). In the MAJIN-TERB1-TERB2 complex, TERB1 probably mediates association with the shelterin/telosome complex via interaction with TERF1, promoting priming telomeric DNA attachment' (PubMed:26548954). Promotes telomere association with the nuclear envelope and deposition of the SUN-KASH/LINC complex (PubMed:24885367, PubMed:24413433). Also recruits cohesin to telomeres to develop structural rigidity (PubMed:24413433).[UniProtKB/Swiss-Prot Function]