Pou2f1 (NM_198932) Mouse Untagged Clone

CAT#: MC221427

Pou2f1 (untagged) - Mouse POU domain, class 2, transcription factor 1 (Pou2f1), transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Pou2f1
• Synonyms: 2810482H01Rik; NF-A1; Oct-1; Oct1; Otf-1; Otf1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC221427 representing NM_198932
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGACGGAGGAGCAGCGAGTCAAGATGAGAGTTCAGCCGCGGCGGCAGCAGCAGCAGACTCAAGAA
  TGAATAATCCATCAGAAACCAATAAATCATCTATGGAGAGTGAAGATGCCAGCACAGGCACACAAACCAA
  TGGTCTGGACTTTCAGAAACAGCCCGTGCCCGTTGGAGGAGCGATCTCCACAGCCCAGGCCCAGGCCTTC
  CTCGGACATCTTCACCAGGTCCAGCTAGCTGGGACAAGTTTACAGGCTGCTGCTCAGTCTTTAAATGTAC
  AGTCTAAATCCAGTGAAGAGTCGGGAGATTCGCAGCAGTCGAGCCAGCCTTCTTCCCAGCCGCCTTCAGT
  GCAGTCAGCCATTCCCCAGACCCAGCTAATGCTGGCTGGGGGACAGATAACTGGGCTCACGTTGACCCCA
  GCCCAGCAACAGTTACTGCTACAGCAGGCGCAGGCCCAGGCCCAGCTCCTGGCCGCTGCAGTGCAGCAAC
  ACTCCGCCAGCCAACAGCACAGTGCTGCTGGGGCCACCATCTCAGCCTCCGCCGCCACACCCATGACGCA
  GATCCCCCTGTCTCAGCCCATACAGATTGCACAGGATCTTCAACAATTGCAACAGCTTCAGCAGCAAAAT
  CTCAACTTGCAACAGTTTGTGTTGGTGCACCCAACCACCAACCTGCAACCAGCACAGTTTATCATCTCAC
  AGACCCCCCAGGGCCAGCAGGGTCTCCTGCAAGCGCAAAATCTTTTAACGCAACTACCTCAGCAAAGCCA
  AGCCAACCTCCTACAGCCACAGCCAAGCATCACCCTCACGTCCCAGCCTACCACCCCAACTCGCACAATA
  GCAGCAGCCTCAGTTCAGACACTTCCACAGAGCCAGTCAACACCAAAGCGAATTGACACTCCCAGCTTGG
  AGGAGCCCAGTGACCTTGAGGAGCTTGAGCAGTTTGCCAAGACTTTCAAACAAAGACGAATCAAACTTGG
  ATTCACTCAGGGTGATGTTGGGCTCGCTATGGGGAAATTATATGGAAATGACTTCAGCCAAACCACCATC
  TCTCGCTTTGAAGCCTTGAACCTCAGCTTTAAGAACATGTGCAAGTTAAAGCCCCTTTTAGAGAAGTGGC
  TAAATGATGCAGAGAACCTCTCATCTGATTCTACAGCATCTAGCCCAAGTGCTTTGAATTCTCCAGGATT
  GGGGGCTGAGGGCTTGAATCGTAGGAGGAAAAAACGCACCAGCATAGAGACCAACATCCGTGTGGCCTTA
  GAGAAGAGTTTCATGGAGAATCAAAAGCCTACCTCGGAAGACATCACCTTGATTGCTGAACAGCTCAATA
  TGGAAAAGGAGGTGATTCGTGTTTGGTTTTGTAACCGCCGCCAGAAGGAGAAAAGAATCAACCCGCCCAG
  CAGTGGTGGGACCAGCAGCTCACCTATCAAAGCAATTTTCCCCAGCCCAGCCTCATTGGTGGCAACCACT
  CCAAGCCTTGTGACAAGCAGTACGGCAACTACCCTCACAGTCAACCCTGTCCTCCCTTTAACCAGTGCTG
  CTGTGACTAATCTCTCTCTTACAGGCACTACAGACTCGACGTCCAACAACAACACGGCCACGGTGATTTC
  CACAGCACCCCCTGCTTCCTCAGCAGTCACATCCCCTTCCTTGAGTCCCTCTCCCTCTGCCTCGGCCTCC
  ACCTCAGAGGCCTCCAGTGCCAGTGAGACCAACACGACACAGACCACCTCCACGCCTCTTCCCTCCCCTC
  TCGGAGCCAGCCAGGTGATGGTGACCACGCCCGGCTTACAGACAGCAGCCGCCGCTCTCCAAGGAGCGGC
  ACAGTTGCCAGCAAACGCCAGTCTTGCTGCTATGGCTGCTGCTGCGGGACTCAGCCCAGGCCTCATGGCA
  CCCTCACAGTTTGCTGCTGGAGGTGCCTTACTCAGTCTCAGTCCGGGGACTCTGGGCAGTGCTCTCAGCC
  CAGCCCTAATGAGCAACAGTACACTGGCAACGATTCAAGCTCTTGCTTCTAGTGGCTCTCTTCCAATAAC
  GTCTCTGGATGCAACTGGGAACCTGGTATTTGCCAATGCAGGAGGAGCCCCGAACATCGTGACTGCACCT
  CTGTTCCTGAACCCTCAGAACCTCTCTCTGCTCACCAGCAACCCAGTAAGCTTGGTTTCTGCCGCTGCAG
  CCTCCACAGGGAACTCTGCACCTACAGCCAGCCTTCATGCCTCCTCCACCTCAACTGAGTCCATCCAGAG
  CTCTCTGTTCACAGTCGCCTCTGCCAGTGGGCCTGCTTCCACCACCACAGCTGCCTCCAAGGCACAGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_198932
• Insert Size: 2310 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_198932.3, NP_945150.2
• RefSeq Size: 12906 bp
• RefSeq ORF: 2310 bp
• Locus ID: 18986
• Cytogenetics: 1 73.21 cM
• Gene Summary: Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3') and activates the promoters of the genes for some small nuclear RNAs (snRNA) and of genes such as those for histone H2B and immunoglobulins. Modulates transcription transactivation by NR3C1, AR and PGR (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) lacks an in-frame exon in the coding region, compared to variant 1. It encodes isoform Z, which is shorter than isoform A. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.